Genetic diversity of partial hrpF and Zur genes in populations ofXanthomonas campestrispv.campestrisinBrassica oleraceaconvar.capitatain the Czech Republic

Eichmeier, Aleš; Čechová, Jana; Peňázová, Eliška

doi:10.17660/actahortic.2015.1105.26

Cited by 4 publications

(10 citation statements)

References 19 publications

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“…Bacteria which infect cruciferous plants are primarily gram-negative. Th e most frequently identifi ed genera are Pseudomonas (Mauzey et al 2015), Erwinia (Togashi et al 2001), Pectobacterium (Nazerian et al 2011), Xanthomonas (Roberts et al 1999;Eichmeier et al 2015) and Bacillus (Agrios 2006). Th e bacteria mainly attack the fl eshy storage organs of their hosts (Bhat et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Rapid Communication. Monitoring the occurrence of bacteria in stored cabbage heads

Eichmeier¹,

Peňázová²,

Pecenka³

et al. 2016

Journal of Plant Protection Research

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Twenty-six cabbage heads stored under typical conditions in a storage hall in Moravia, Czech Republic, were tested for the presence of bacteria by the method of isolation from three diff erent parts of the cabbage heads. Isolations were carried out from stalks, inner and superfi cial leaves. Two samplings were done; in November 2015 and February 2016. Bacterial cultures were sequenced in the part of 16S rRNA region; bacteria were identifi ed according to the sequences obtained. Th e most prevalent bacteria were of the genus Pseudomonas. Genera: Klebsiella, Erwinia, Pantoea, Bacillus were also identifi ed. Th e results provided an interesting insight into the bacterial spectrum in stored cabbage heads and their dynamics during storage. Th e nucleotide sequences which were found were saved in GenBank/NCBI under accession numbers KX160104-KX160145.

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Section: Introductionmentioning

confidence: 99%

Rapid Communication. Monitoring the occurrence of bacteria in stored cabbage heads

Eichmeier¹,

Peňázová²,

Pecenka³

et al. 2016

Journal of Plant Protection Research

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“…PCR products were sequenced according to Eichmeier et al [24] in order to validate the amplicon sequences. For pre-amplification, primer pair Zur1-EAC-fwd and Zur1-CAE-rev [25] (Table 1) were used, targeting the Zinc uptake regulator (Zur) gene, with product length of 305 bp. The reaction mixture was the same as previously described for the hrpF fragment.…”

Section: Conventional Pcr Detection Of the Hrpf Genementioning

confidence: 99%

“…Primer pair Zur2-EAC-fwd and Zur1-CAE-rev, and a Zur1-TP probe, designed by Eichmeier et al [18,25] (Table 1), were used for specific amplification of a short region of the Zur gene using a TaqMan ® Real Time PCR system (MCLAB, San Francisco, SF, USA). The targeted gene region lies within the region targeted by the primer pair used in the pre-amplification (Zur1-EAC-fwd and Zur1-CAE-rev) with a product length of 142 bp.…”

Section: Quantitative Real-time Pcrmentioning

confidence: 99%

“…The intercept was 23.8 when log 10 (CFU) = 0 (i.e., CFU of Xcc in the PCR tube = 1). As it makes no sense to be able to detect less than a single bacterial cell in a tube, and the Zur gene copy number per cell has been estimated as one [25], we therefore took this value to be the cutoff for detection of Xcc based on the real-time PCR (Table A1).…”

Section: Estimation Of Xcc By Inverse Estimation Using Nested Real-time Pcr Ct Valuesmentioning

confidence: 99%

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Persistence of Xanthomonas campestris pv. campestris in Field Soil in Central Europe

Gazdík

Magnus

Roberts³

et al. 2021

Microorganisms

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Xanthomonas campestris pv. campestris (Xcc) is a bacterium that causes black rot of crucifers. The greatest losses of brassica crop production usually result from seed-borne infection, but carry-over of inoculum in field soil may also be possible. The aim of this study was to monitor persistence of Xcc in field soil in central Europe using a conventional PCR assay with hrpF primers and a two-step nested real-time PCR assay using Zur primers. The work has demonstrated that nested real-time PCR can be used to improve the analytical sensitivity for detection of Xcc in soil compared to conventional PCR, and that Xcc may persist in soil for up to two years following an infected brassica crop in central European climatic conditions.

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“…The standard PCR targeting the hrpF (hypersensitivity reaction and pathogenicity) gene was used. The reaction was performed as described by Eichmeier et al (2015) using primer pair DLH 120 -125 (619 bp, Berg et al 2005). The PCR was done primarily for determination of Xcc spreading in plants.…”

Section: Disease Assessmentmentioning

confidence: 99%

Testing of Inoculation Methods and Susceptibility Testing of Perspective Cabbage Breeding Lines (Brassica Oleracea convar. Capitata) to the Black Rot Disease Caused by Xanthomonas Campestris pv. Campestris

Peňázová¹,

Kopta²,

Jurica³

et al. 2018

Acta Univ. Agric. Silvic. Mendelianae Brun.

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The susceptibility of twenty-four cabbage breeding lines to Xanthomonas campestris pv. campestris was evaluated. The selection of appropriate inoculation method was done on 4 cabbage cultivars ('Cerox', 'Sintex', 'Sonja' and 'Avak'). One month old plants were infected by 5 inoculation methods (spraying, injection by syringe, multiple pricking, carborundum abrasion and scissor clipping method). Four different bacterial isolates of Xcc (WHRI 3811, 3971A, 1279A; SU) and their mixture were evaluated for the aggressiveness on 'Cerox' and 'Sonja' cultivars. On the basis of obtained results, breeding lines of head cabbage were inoculated by mixture of all tested isolates using multiple pricking method. The disease severity of inoculated seedlings proved high susceptibility of young plants to the Xcc infection. The disease incidence determined 75 and 105 days after sowing showed changes for 16 of tested lines and indicated that resistance testing should be observed until mature stage. The study revealed five breeding lines (DP25, T1, IT10, Kalibos and Avak1) with disease incidence lower than 20 % as perspective sources of resistance for further breeding.

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Genetic diversity of partial hrpF and Zur genes in populations ofXanthomonas campestrispv.campestrisinBrassica oleraceaconvar.capitatain the Czech Republic

Cited by 4 publications

References 19 publications

Rapid Communication. Monitoring the occurrence of bacteria in stored cabbage heads

Rapid Communication. Monitoring the occurrence of bacteria in stored cabbage heads

Persistence of Xanthomonas campestris pv. campestris in Field Soil in Central Europe

Testing of Inoculation Methods and Susceptibility Testing of Perspective Cabbage Breeding Lines (Brassica Oleracea convar. Capitata) to the Black Rot Disease Caused by Xanthomonas Campestris pv. Campestris

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