Persistence of Xanthomonas campestris pv. campestris in Field Soil in Central Europe

Abstract

Xanthomonas campestris pv. campestris (Xcc) is a bacterium that causes black rot of crucifers. The greatest losses of brassica crop production usually result from seed-borne infection, but carry-over of inoculum in field soil may also be possible. The aim of this study was to monitor persistence of Xcc in field soil in central Europe using a conventional PCR assay with hrpF primers and a two-step nested real-time PCR assay using Zur primers. The work has demonstrated that nested real-time PCR can be used to improve the analytical sensitivity for detection of Xcc in soil compared to conventional PCR, and that Xcc may persist in soil for up to two years following an infected brassica crop in central European climatic conditions.

Integrating Science on Xanthomonas and Xylella for Integrated Plant Disease Management

“…A pivotal eco-environmental aspect of X. campestris pv. campestris , the causal agent of the black rot of Crucifers, was described by Gazdik et al [ 18 ]. They monitored the persistence of the bacterial inoculum in the cropped field, discovering that the pathogen is able to survive at least two years in cabbage fields.…”

Identification of black rot caused by Xanthomonas campestris pv. campestris and evaluation of seed treatments for alternative control methods

“…Moreover, the bacteria were reported and caused losses on certificated and hybrid Brassica seeds (van Zijll de Jong et al 2010: Little 2021. The pathogens can survive in cruciferous weed hosts, seeds, and infected crop residues in the soil which are created main source of inoculum (Lopez et al 1999: Gazdik et al 2021). Therefore, using healthy propagating material such as Xcc-free seed can help to prevent or suppress the spreading of the pathogen and decrease yield losses.…”

Isolation of Novel Xanthomonas Phages Infecting the Plant Pathogens X. translucens and X. campestris

“…The rod-shaped uniflagellar yellow colony forming bacteria causes V-shaped lesions in many Brassicaceae plants, of which B. oleracea (cabbage, cauliflower, and broccoli) is the economically most relevant [ 29 , 30 ]. Its main route of transmission is via the seeds, but it also was shown to be persistent in field soil [ 31 ]. For Xcc, phage isolates have been reported in the past (Caudovirales: FoX 1–7 [ 32 ]; phage PPDBI, phage PBR31, ϕL7, Phage Carpasina [ 33 ], XC1, Xccφ1 [ 34 ], Tubuvirales: ϕLf2, ϕLF), but there is still a great need for further phage isolates applicable for biocontrol.…”