Rapid Communication. Monitoring the occurrence of bacteria in stored cabbage heads

Abstract

Twenty-six cabbage heads stored under typical conditions in a storage hall in Moravia, Czech Republic, were tested for the presence of bacteria by the method of isolation from three diff erent parts of the cabbage heads. Isolations were carried out from stalks, inner and superfi cial leaves. Two samplings were done; in November 2015 and February 2016. Bacterial cultures were sequenced in the part of 16S rRNA region; bacteria were identifi ed according to the sequences obtained. Th e most prevalent bacteria were of the genus Pseudomonas. Genera: Klebsiella, Erwinia, Pantoea, Bacillus were also identifi ed. Th e results provided an interesting insight into the bacterial spectrum in stored cabbage heads and their dynamics during storage. Th e nucleotide sequences which were found were saved in GenBank/NCBI under accession numbers KX160104-KX160145.

The Change of Bacterial Spectrum after Storage of X. campestris pv. campestris Inoculated Cabbage Heads (Brassica oleracea var. capitata L.)

“…Pseudomonas species appeared more on cabbage heads at harvest, however, the bacterial spectrum changed over time in favor of this genus. The decline of other bacterial genera after four months of cold storage was also observed in our previous study [21] and it could be explained by strong antagonistic behavior of Pseudomonas sp. [46] and Bacillus species against other bacteria [47,48].…”

“…In this study, the presence of the B. subtilis group in control heads was similar in both samplings and detection of the B. cereus group decreased markedly after storage. However, in our previous studies, the increase of the latter genus during storage was observed [21]. Species belonging to Bacillus genera are inconsistently reported as food poisoning microorganisms frequently present in cooked and slowly cooled-down food [55,56], while on the other hand some are considered as potential bio-control agents against postharvest pathogens of vegetables and fruits [48,57].…”

“…The use of proper packing material (e.g., alum, newspaper wrap) can reduce mechanical damage particularly during transport, thus decreasing the incidence and spread of bacterial soft rot pathogens [3]. According to several authors, the antagonistic behavior of Pseudomonas and Bacillus species against other bacterial genera has been presumed [21][22][23]. Antagonistic effects of Bacillus sp.…”

Pantoea stewartii subsp. stewartii, the causative agent of Thai jackfruit’s bronzing disease and its possible host range in Vietnam

“…The Pss pathogen isolated from bronzing jackfruit in Malaysia could affect sweetcorn, pineapple, and cucumber in vitro (Abidin et al 2021). In addition, other Pantoea species are severe pathogens of rice that cause diseases worldwide (Azizi et al 2020), as well as on other crops such as strawberries (Abdel-Gaied et al 2022) in the field and cabbage heads in storage (Eichmeier et al 2017).…”