Genetic Diversity of Norovirus and Sapovirus in Hospitalized Infants with Sporadic Cases of Acute Gastroenteritis in Chiang Mai, Thailand

Hansman, Grant S.; Katayama, Kazuhiko; Maneekarn, Niwat; Peerakome, Supatra; Khamrin, Pattara; Tonusin, Supin; Okitsu, Shoko; Nishio, Osamu; Takeda, Naokazu; Ushijima, Hiroshi

doi:10.1128/jcm.42.3.1305-1307.2004

Cited by 137 publications

(137 citation statements)

References 19 publications

(16 reference statements)

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“…The rate of SaVs obtained in Belém was similar to those reported in Thailand and Australia (4.8% and 4.1%, respectively) and lower than rates in India 20,21,35 (10.2%)…”

Section: Fromsupporting

confidence: 59%

Molecular characterization of norovirus, sapovirus and astrovirus in children with acute gastroenteritis from Belém, Pará, Brazil

et al. 2010

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Molecular characterization of norovirus, sapovirus and astrovirus in children with acute gastroenteritis from Belém, Pará, Brazil Caracterização molecular de norovírus, sapovírus e astrovírus em crianças com gastroenterite aguda em Belém, Pará, Brasil Caracterización molecular de norovirus, sapovirus y astrovirus en niños con gastroenteritis aguda en Belém (Pará, Brasil) http://revista.iec.pa.gov.br Glicélia Cruz Aragão ABSTRACTThe importance of norovirus (NoVs), sapovirus (SaVs) and human astrovirus (HAstVs) as causes of gastroenteritis outbreaks is already well-defined, but a few studies have described sporadic cases of acute gastroenteritis caused by these viral entities. The aim of this study was to determine the role of these viruses in the etiology of acute gastroenteritis in children enrolled to participate in hospital -and emergency department -based intensive surveillance carried out in Belém, Brazil, from March to September 2003. A total of 305 stool specimens from patients with severe gastroenteritis were collected and screened by reverse transcription followed by polymerase chain reaction (RT-PCR), using the specific primers Mon 269 and Mon 270 for HAstVs, p289 and p290 for human calicivirus (HuCVs), and Mon 431/433 and Mon 432/434 for NoVs. Sequencing of RT-PCR HAstV, HuCV and NoV amplicons was carried out using the same primers. Of the 305 samples tested, 96 (31.5%) were positive, with 51 diagnosed as HuCVs, 40 as HAstVs and five as mixed infections. Of the 56 (18.4%) HuCVs sequenced, 30 were NoVs (9.8%) of genogroups and 15 (4.9%) were SaVs of types GI-1, GI-2 and GII-1. HAstVs, including genotypes 1, 8 and 2, were detected in 45 (14.7%) samples. This study has highlighted the importance of these viruses as causes of acute gastroenteritis and established the circulation of different genotypes during the study period. These results reinforce the need for establishing an intensive surveillance for gastroenteritis caused by these viruses to assess the burden of disease and to monitor the circulation of genotypes.

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“…The rate of SaVs obtained in Belém was similar to those reported in Thailand and Australia (4.8% and 4.1%, respectively) and lower than rates in India 20,21,35 (10.2%)…”

Section: Fromsupporting

confidence: 59%

Molecular characterization of norovirus, sapovirus and astrovirus in children with acute gastroenteritis from Belém, Pará, Brazil

et al. 2010

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“…A similar occurrence of these viruses in male and female children in different age groups is described in the literature (Kaplan et al 1982) although there is a higher rate of occurrence of these viruses in children up to 12 months of age (Pang et al 1999, Rockx et al 2002, Hansman et al 2004.…”

Section: Discussionmentioning

confidence: 70%

Detection of calicivirus from fecal samples from children with acute gastroenteritis in the West Central region of Brazil

Borges¹,

Teixeira

Costa

et al. 2006

Mem. Inst. Oswaldo Cruz

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“…The results from the hospital population are consistent with those from similar studies conducted in other developing and developed countries. In a report from Thailand, 12% of stool specimens were positive for NoVs [Hansman et al, 2004a]. Reports from Malawi, China, Vietnam, Mongolia, and India [Kang et al, 2000;Hansman et al, 2004bHansman et al, , 2005Dove et al, 2005;Liu et al, 2006], have reported NoVs associated with 6-25% of acute gastroenteritis cases.…”

Section: Discussionmentioning

confidence: 99%

Human caliciviruses in symptomatic and asymptomatic infections in children in Vellore, South India

Monica

Ramani

Banerjee

et al. 2007

Journal of Medical Virology

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Pediatric gastroenteritis is a major cause of childhood morbidity and mortality worldwide, especially in developing countries. It has been increasingly recognised that human caliciviruses (HuCV), comprising noroviruses (NoV), and sapoviruses (SaV), are important in both outbreak and non-outbreak settings. This study aimed to characterise caliciviruses detected in the faeces of hospitalized children and children in the community in India. This study examined 350 faecal samples from children presenting to the hospital with acute gastroenteritis and 673 samples collected from children in the community, 500 from children with diarrhea, and 173 samples from children without diarrhea. Strain characterisation was performed by reverse transcriptionpolymerase chain reaction (RT-PCR) and partial sequencing of the gene encoding the RNAdependent RNA polymerase (RdRp) and/or a region spanning the open reading frames (ORFs) 1 and 2 (ORF1/ORF2) junction. A total of 68 of 350 specimens (19.4%) from hospitalized children were positive, and SaV and NoV accounted for 5.1 and 15.1% of the infections, respectively. Mixed infections of HuCVs with other enteric pathogens were seen in 9.4% of the total children tested. Sixty-eight out of 673 (10.1%) samples collected from children in the community were positive for caliciviruses, and SaV and NoV accounted for 3.4 and 6.6% of the infections. In the community cohort 55/500 (11%) and 13/173 (7.5%) were from symptomatic and asymptomatic children, respectively, and SaVs accounted for 17/500 (3.4%) and NoVs for 38/500 (7.6%) of the symptomatic infections. This is the first report of genotyping of circulating caliciviruses in both hospital and community in India and has increased the evidence for the role of these viruses in pediatric gastroenteritis in India.

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Genetic Diversity of Norovirus and Sapovirus in Hospitalized Infants with Sporadic Cases of Acute Gastroenteritis in Chiang Mai, Thailand

Cited by 137 publications

References 19 publications

Molecular characterization of norovirus, sapovirus and astrovirus in children with acute gastroenteritis from Belém, Pará, Brazil

Molecular characterization of norovirus, sapovirus and astrovirus in children with acute gastroenteritis from Belém, Pará, Brazil

Detection of calicivirus from fecal samples from children with acute gastroenteritis in the West Central region of Brazil

Human caliciviruses in symptomatic and asymptomatic infections in children in Vellore, South India

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