Genetic diversity of Brazilian pig breeds evidenced by microsatellite markers

Sollero, B. P.; Paiva, Samuel Rezende; Faria, Danielle Assis de; Guimarães, Simone Eliza Facioni; Castro, Sílvia; Egito, A. A. do; Albuquerque, M. S. M.; Piovezan, Ubiratan; Bertani, Giovani R.; Mariante, A. da S.

doi:10.1016/j.livsci.2008.09.025

Cited by 53 publications

(47 citation statements)

References 35 publications

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“…These values are similar to those reported in Denmark and the Netherlands and are exceeded by those reported in China, Brazil and Thailand (25)(26)(27)(28).…”

Section: Discussionsupporting

confidence: 88%

Genetic characterization of the domestic pig (Sus scrofa domestica) in Cerete-Colombia, using microsatellite markers

2014

Rev MVZ Córdoba

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Objective. The purpose of this study was to characterize a population of domestic pig (Sus scrofa domestica) in Cereté, Córdoba, using 20 microsatellite; calculate heterozygosity per locus and average heterozygosity. Materials and methods. Hair samples were collected from 62 specimens. DNA was extracted by proteinase K digestion and phenol-chloroform purification. Information from 20 microsatellites was selected out of those recommended for swine biodiversity studies. PCR products were separated by a vertical polyacrylamide gel electrophoresis. The bands were visualized by staining with silver nitrate. Results. All microsatellites used were polymorphic. Between 3 (SW1067) and 15 (IFNG) alleles were detected with an average number of 6.7 and a total de 134 alleles. The average expected and observed heterozygosities were 0.5278 and 0.5479, respectively. PIC values ranged between 0.1999 and 0.8300 for loci SW1067 and SW911, respectively. Conclusions. Levels of observed and expected heterozygosity found in the present study indicate that the domestic pig (Sus scrofa domestica) in Córdoba Cereté show high degree of genetic variability Key words. Alleles, genetic diversity, heterozygosity (Source: DeCS). RESUMENObjetivo. El objetivo del presente estudio fue caracterizar una población de cerdo doméstico (Sus scrofa domestica) en Cereté, Córdoba utilizando 20 microsatélites; calcular la heterocigosidad por locus y la heterocigosidad media. Materiales y métodos. Se recolectaron muestras de bulbos de pelo de 62 ejemplares. El ADN se extrajo mediante digestión con proteinasa K y una purificación con fenol-cloroformo. Se utilizó la información proporcionada por 20 marcadores microsatélites de los recomendados para estudios de biodiversidad porcina. Los productos de PCR se separaron mediante electroforesis vertical en gel de poliacrilamida. Las bandas se visualizaron por tinción con nitrato de plata Resultados. Todos los microsatélites utilizados fueron polimórficos. Se detectaron, entre 3 (SW1067) y 15 (IFNG) alelos, con un número medio de alelos de 6.7 y un total de 134. Las heterocigosidades media esperadas y observadas fueron 0.5278 y 0.5479 respectivamente. Los valores del PIC oscilaron INTRODUCCIÓNEl cerdo doméstico (Sus scrofa domestica) es un mamífero cuadrúpedo perteneciente al grupo Suinos, del genéro Sus (Familia Suidae), que se caracteriza por tener cuatro dedos terminados en forma de pezuña; el segundo y el tercer dedo le sirven de apoyo y el primero y cuarto no los apoya. La posesión de cuatro pezuñas, lo coloca en el orden de los artiodáctilos.Su cabeza es larga y gruesa, en las razas comunes es de forma piramidal. Su cráneo es sólido; en los animales jóvenes las regiones nasal y frontal son rectas, con el tiempo se hacen cóncavas. El hocico es de forma cilíndrica con un borde abultado; es lampiño y liso; los orificios nasales son pequeños y sus ojos son de pupilas redondas.Los cerdos domésticos del continente americano tienen su origen en los cerdos ibéricos traídos por Colón en su segundo viaje (1)....

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“…These values are similar to those reported in Denmark and the Netherlands and are exceeded by those reported in China, Brazil and Thailand (25)(26)(27)(28).…”

Section: Discussionsupporting

confidence: 88%

Genetic characterization of the domestic pig (Sus scrofa domestica) in Cerete-Colombia, using microsatellite markers

2014

Rev MVZ Córdoba

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“…However, according Mariante et al (2003) the Piau breed is threatened. In addition, Sollero et al (2009) found a high rate of inbreeding in two Brazilian herds of Piau pigs using DNA microsatellite analysis.…”

Section: Introductionmentioning

confidence: 99%

“…The genetics underpinnings of the large fat deposition of Piau pigs have been explored in genetic studies by the Animal Breeding and Genetics group of the Federal University of Viçosa, Minas Gerais state, Brazil. These studies include QTL mapping using divergent crosses Paixão et al, 2008;Silva et al, 2008), polymorphism analysis of candidate genes (Band et al, 2005;Carmo et al, 2005;Faria et al, 2006;Peixoto et al, 2006), phylogeny and genetic diversity analyses (Sollero et al, 2009;Souza et al, 2009) and expression patterns of candidate genes (Sousa et al, 2011;Serão et al, 2011;Sollero et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Using pedigree analysis to monitor the local Piau pig breed conservation program

Veroneze¹,

Lopes²,

Guimarães³

et al. 2014

Arch. zootec.

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“…Several studies have successfully used microsatellite molecular markers to characterize the genetic diversity between breeds and species of livestock (Li et al, 2000;Baumung et al, 2006;Yang et al, 2008;Sollero et al, 2008;Arora et al, 2010;Kugonza et al, 2011;Dixit et al, 2012;Souza et al, 2012) because these markers have high polymorphism, co-dominant inheritance, abundance in the genome, selectively neutral behavior, easy access, and high reproducibility (Dodgson et al, 1997).…”

Section: Introductionmentioning

confidence: 99%