Genetic diversity for RAPD markers between cultivated and wild accessions of Coffea arabica

Lebailly, Philippe; Trouslot, Pierre; Anthony, François; Combes, Marie‐Christine; Charrier, André

doi:10.1007/bf00022965

Cited by 132 publications

(106 citation statements)

References 9 publications

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“…O estudo da diversidade genética em café, utilizando marcadores moleculares do tipo RAPD, comprovou a pequena diversidade genética existente entre as cultivares (OROZCO et al, 1994;LASHERMES et al, 1996).…”

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Avaliação de progênies obtidas de cruzamentos de descendentes do Híbrido de Timor com as cultivares Catuaí Vermelho e Catuaí Amarelo

et al. 2004

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RESUMOAvaliou-se com este trabalho o comportamento de 28 progênies F3 de Coffea arabica obtidas de cruzamentos das cultivares Catuaí Vermelho e Catuaí Amarelo com descendentes do Híbrido de Timor, realizados pela Epamig em conjunto com a UFV. Estimaram-se os parâmetros genéticos e as correlações entre caracteres, buscando conhecer a estrutura genética da população e seu potencial para o melhoramento. O experimento foi instalado em Patrocínio, Estado de Minas Gerais, em delineamento de blocos casualizados com seis repetições, contendo 28 progênies F3 e duas testemunhas da cultivar Catuaí Vermelho IAC 15. Analisaram-se as produções obtidas nas quatro colheitas iniciais, de 1997 a 2000 e alguns caracteres vegetativos. As progênies avaliadas apresentaram média de produção de grãos superior à testemunha, e grande variabilidade genética, sugerindo a possibilidade de se obter linhagens superiores. As progênies avaliadas apresentaram-se resistentes às raças fisiológicas da ferrugem presentes na região do experimento. A progênie 505-9-2 se destaca como material produtivo e vigoroso e de porte alto, enquanto as progênies 514-7-10 e 514-7-6 além de produtivas e vigorosas apresentaram porte baixo semelhante à cultivar Catuaí Vermelho IAC 15. A produção de grãos apresentou correlação genotípica alta e positiva com os caracteres diâmetro do caule, vigor, porte, altura e diâmetro da planta, mas não apresentou resultados consistentes de correlação com carga pendente. A produção de grãos de anos de colheita mostrou-se correlacionada com a produção total de quatro anos apenas a partir do segundo ano de produção.Palavras-chave: café, Coffea arabica L., melhoramento do cafeeiro, Híbrido de Timor.

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Avaliação de progênies obtidas de cruzamentos de descendentes do Híbrido de Timor com as cultivares Catuaí Vermelho e Catuaí Amarelo

et al. 2004

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“…These cultivars were not distinguished among one another however, such cultivars did not belong to the same genetic origin, therefore they were not genetically identicals, as showed. These results suggested that RAPD markers were not efficient enought to distinct C. arabica cultivars as detected by Lashermes et al (1993Lashermes et al ( ,1996 and Anthony et al (2001), who reported that the low level of molecular polymorphism detected in C. arabica cultivars was most probably related to the predominant autogamy, the recent allotetraploid origin of the species that involve a limited number of plants from which the cultivars were selected. On the other hand, Obata (Sarchimor) was the cultivar that showed the highest genetic distance, closer to Sarchimor group [IPR99, IAPAR59 (L105), IAPAR 59 (L104)], as expected.…”

Section: Resultsmentioning

confidence: 86%

“…The PCR procedure was performed with 25 ng of genomic DNA, 2.5 mM buffer 10X [200 mM Tris (pH 8.4), 500 mM KCL], gelatin 0.01%, 1.5 mM MgCl 2, 1.25 mM dNTP, 5 picomols of primer (decamer oligonucleotide purchased from Operon Technologies Inc. -OPA 01, OPA 04, OPG 11, OPY 16 and OPX 09), 1 U of Taq polymerase and ultra pure water up to 25 µL. The amplifications were performed in a thermocycler PTC 100 TM (MJ Research), using the temperature program described by Lashermes et al (1996): 1 cycle of 4 min. at 94ºC followed by 43 cycles of 1 min.…”

Section: Methodsmentioning

confidence: 99%

“…bourbon were clearly distinguished by RAPD markers (Orozco-Castillo et al, 1994). RAPD method appeared to be effective in resolving genetic variations between cultivated, wild accessions and in distinct groups of C. arabica germplasm, suggesting an East-West differentiation in Ethiopia, the primary centre of diversification of this species (Lashermes et al, 1996). The aim of this research was to identify and to distinguish lines and cultivars of C. arabica of commercial interest using RAPD markers.…”

Section: Introductionmentioning

confidence: 99%

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Varietal identification of coffee seeds by RAPD technique

Crochemore

Nunes

Andrade

et al. 2004

Braz. arch. biol. technol.

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“…In view of this, characterization and evaluation of its gene pool is necessary for effective crop improvement programmes and for better conservation and management of genetic resources [4]. Assessment of genetic variability within and among arabica coffee populations using molecular markers such as RAPD, ISSR, ALFP, and SSR has been the subject of several studies [5][6][7][8][9]. Similar analysis in C. canephora has paramount importance, especially for genetic materials from Brazil.…”

Section: Introductionmentioning

confidence: 99%

SNPs Based Molecular Diversity of Coffea canephora

Bikila¹,

Sakiyama²,

Caixeta³

2017

JMEN

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Coffee offers one of the most widely drunk beverages in the world, and is a very important source of foreign currency income for many countries. Coffea canephora Pierre presents a great genetic variability, with one of the widest natural geographical distribution within the subgenus. Newly developed single nucleotide Polymorphism (SNP) markers are effective in genetic diversity detection. The objective of this study was to analyze the genetic diversity and group C. canephora accessions, which were genotyped with SNPs molecular markers. In the present study, C. canephora germplasm consisting of 50 clones (24 Conilon and 26 Robusta) were used. Genetic diversity was investigated using 46074 polymorphic SNP markers covering the entire genome of C. canephora. The estimation of genetic similarity between each pair of individuals was calculated by the Jaccard coefficient using the program NTSYS pc2.1. A simplified representation of the similarity was obtained by constructing the dendrogram using UPGMA method (Unweighted Pair-Group Method using Arithmetic Mean). The optimal number of groups in the dendrogram was determined by the relative size of distances in the dendrogram. Thus, the first group was composed of clones 1, 5, 24, 6, 9, 14, 23, 22, 10, 17, 19, 18, 20, 2, 13, 11, 15, 7, 4 , 3 and 21; the second group 8, the third group 12, the fourth 16, the fifth group 26, 31, 33, 34, 37, 38, 28, 45, 46, 41 25, 29, 36, 43, 48, 49, 47, 50, 35, 39, 44, 40, 32, 30, and the sixth composed by 27 and 42. Generally, the analysis showed that the C. canephora clones were clearly divided into diversity groups that can be used for further breeding programs.

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Genetic diversity for RAPD markers between cultivated and wild accessions of Coffea arabica

Cited by 132 publications

References 9 publications

Avaliação de progênies obtidas de cruzamentos de descendentes do Híbrido de Timor com as cultivares Catuaí Vermelho e Catuaí Amarelo

Avaliação de progênies obtidas de cruzamentos de descendentes do Híbrido de Timor com as cultivares Catuaí Vermelho e Catuaí Amarelo

Varietal identification of coffee seeds by RAPD technique

SNPs Based Molecular Diversity of Coffea canephora

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