Genetic diversity and zoonotic potential of human rotavirus strains, 2003–2006, hungary

Abstract

Rotavirus strain surveillance is being conducted in many countries before and after introduction of newly licensed vaccines to assess the impact of the vaccines on rotavirus strains. Here we describe a strain surveillance study in the Budapest area of Hungary (2003-2006) based on RNA profile analysis, genotyping by multiplex PCR and nucleotide sequencing. Among 1,983 G-typed rotaviruses we identified G1 (22%), G2 (4.8%), G3 (3.5%), G4 (18.5%), G6 (1.1%), G8 (<0.1%, n = 1), G9 (42%), and G12 (3.4%) specificities. Information on P genotype incidence was determined for a subset of samples (n = 814). In addition to the globally important strains, a variety of uncommon antigen combinations were also found, for example, P[9],G3; P[14],G6; or P[14],G8. Sequence and phylogenetic analysis of the VP7, VP4, VP6, and NSP4 genes of selected strains with uncommon antigen combinations demonstrated high similarity with certain bovine, porcine, feline, equine, and lapine rotaviruses, respectively. Continued surveillance is needed to assess the role of animal rotaviruses in human diseases.

Molecular characterization of group A bovine rotavirus in southeastern and central-western Brazil, 2009-2010

“…Hussein et al's study (1993) conϐirmed the existence of the G11 genotype in samples of bovine rotavirus and other uncommon strains in the United States. The participation of genotype G11, which is typically considered porcine and has also been identiϐied in humans (Bányai et al 2009, Rahman et al 2005, Shim et al 2011, was also observed in the present work. This ϐinding can be considered of major epidemiological signiϐicance in view of the scanty reports of this genotype in Brazilian cattle, and may be a result of sharinf of the same environment by cattle and pigs.…”

Molecular Epidemiology of Rotavirus in Cats in the United Kingdom

“…Both G3P [9] and G6P [9] genotypes have been isolated from people in other parts of the globe. Human G3P [9] is considered to be a direct result of transmission events from canine or feline rotaviruses and has been reported in human clinical surveillance samples worldwide, including Japan (13), Israel (56), Brazil (57), Thailand (58), Russia (59), and Hungary (60). G6P [9] was originally isolated from an Italian child with diarrhea (61) and has subsequently been reported in the United States (62), Hungary (60,63), Japan (64), Australia (65,66), and Tunisia (16).…”

Rotavirus genotypes in children in the Basque Country (northern Spain) over a 13-year period (July 1996–June 2009)

“…Three strains belonging to G-types 6 (G6[P14]) and 8 (G8[P8] and G8 [P14]) were detected. G6 and G8 strains are frequently found in bovine cattle [9] and are rarely detected in human infections in Europe [14,15,18,21,25,26]. Human [P14] rotavirus strains have been reported exceptionally across the globe and probably represent occasional interspecies transmission from ruminants [28].…”