Molecular characterization of group A bovine rotavirus in southeastern and central-western Brazil, 2009-2010

Abstract

<jats:p>Rotavirus is an important cause of neonatal diarrhea in humans and several animal species, including calves. A study was conducted to examine 792 fecal samples collected from calves among 65 dairy and beef herds distributed in two of Brazil's major livestock producing regions, aiming to detect the occurrence of rotavirus and perform a molecular characterization of the rotavirus according to G and P genotypes in these regions. A total of 40 (5.05%) samples tested positive for rotavirus by the polyacrylamide gel electrophoresis (PAGE) technique. The molecular characterization was performed by multiplex semi-nested RT-PCR reactions, which indicated that the associations of genotypes circulating in herds in Brazil's southeastern region were G6P[11], G10P[11], G[-]P[5] + [11], G[-]P[6] in the state of São Paulo and G6P[11], G8P[5], G11P[11], G10P[11] in the state of Minas Gerais. In the central-western region, the genotypes G6P[5] + [11], G6P[5], G8P[-], G6P[11], G [-] P[1], G[-] P[11], and G[-] P[5] were detected in the state of Goiás, while the genotypes G6P[5], G8[P11], G6[P11], G8[P1], G8[P5], G6[P1] were circulating in herds in the state of Mato Grosso do Sul. The genotypic diversity of bovine rotavirus found in each region under study underlines the importance of characterizing the circulating samples in order to devise the most effective prophylactic measures.</jats:p>

Analysis of bovine rotavirus strains circulating in diarrheic dairy calves in Uberaba, Minas Gerais, Brazil, during 2008-2009

“…The rotavirus detection rate found in this study (6.8%) was similar to that (5%) observed in another study conducted in Brazil (Silva et al, 2012), but lower than the average (33.7%) determined in a recent review that included studies from five continents (Papp et al, 2013). The variations in prevalence rate may be a reflection of the differences between age, hygiene, and management conditions of animals in the studies.…”

Whole-genomic analysis of 12 porcine group A rotaviruses isolated from symptomatic piglets in Brazil during the years of 2012–2013

“…For instance, reassortment events between porcine and human G11 RVA strains have been identified recently (Shetty et al, 2014;Than et al, 2013). In Brazil, genotype G11 has also been identified in RVAs isolated from cows (Silva et al, 2012), suggesting the occurrence of reassortment events and potential cross-infection of the virus due to proximity between farm-raised cattle and pigs.…”

Severe diarrhea outbreak in beef calves (Bos indicus) caused by G6P[11], an emergent genotype of bovine rotavirus group A

“…Some cross-sectional epidemiological surveys for the determination of the most frequent BoRVA genotypes done in stored fecal samples have identified the combination G6P [11] in detection rates that varied from 2% (1/34) to 34.3% (12/35) of the samples analyzed , Buzinaro et al 2009, Caruzo et al 2010, Silva et al 2012. However, this is the first report of a severe neonatal diarrhea outbreak caused by a single infection of the G6P[11] genotype in Nelore calves (Bos indicus).…”

“…In Brazilian cattle herds the BoRVA genotype G6P [11] was only identified in diarrheic fecal samples in lower frequency in cross-sectional epidemiological studies (Brito et al 2000, Buzinaro et al 2009, Caruzo et al 2010, Silva et al 2012. However, there are no reports of the G6P[11] genotype involvement as a single causative agent of diarrheal outbreak in Brazilian beef cattle herds.…”