Genetic diversity and variation of Chinese fir from Fujian province and Taiwan, China, based on ISSR markers

Yu, Chang Yeon; Peng, Zhuqing; Chao, Wu; Ma, Zhihui; Ding, Guochang; Cao, Gaoshao; Ruan, Si-Min; Lin, Shan-Hsiung

doi:10.1371/journal.pone.0175571

Cited by 17 publications

(17 citation statements)

References 39 publications

(50 reference statements)

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“…In the same concern Žiarovská et al 30 indicated that the different ISSR fingerprint patterns of the amaranth mutant lines when compared to the Ficha cultivar and K-433 hybrid, ISSR specific profiles may be part of a consequence of the complex response of the intergenic space of mutant lines to the gamma-radiance. Genetic analysis using ISSR of the different populations of Chinese fir in Fujian province exhibited a greater level of genetic diversity than that generated from the populations in Taiwan 31 . Polylocus ISSR-PCR markers can be used for characterizing gene pools and for molecular genetics identification of populations and breeds, including starlet populations and replacement brood stocks 32 .…”

Section: Primer Namementioning

confidence: 91%

Physiological and molecular studies on ISSR in two wheat cultivars after exposing to gamma radiation

Aly¹,

Eliwa²,

Maraei³

2019

ScienceAsia

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Grains of two bread wheat cultivars (Triticum aestivum), Sids-1 and Sakha-93, were irradiated with gamma rays at dose levels (0.0, 100, 200, and 300 Gy) to study the effects of gamma irradiation on physiological characteristics and genetic variation of wheat. Irradiation dose level of 200 Gy increased chlorophyll a, b, and total chlorophyll contents significantly in both cultivars. Also, proline content increased with increasing irradiation dose level, the highest concentrations were recorded at 300 Gy for Sids-1 and Sakha-93 cultivars as compared to the control. Inter-Simple Sequence Repeat (ISSR) markers have been done to help understanding their genetic differences. Eight ISSR primers (14A, 44B, HB-08, HB-10, HB-11, HB-12, HB-13, and HB-15) exhibited polymorphism with the un-irradiated and irradiated two wheat cultivars. These pimers successfully showed different banding patterns with several amplicons varied from 4 for (14A) to 15 for (HB-10). These 72 amplicons for the two cultivars and an average of 9.0 amplicons with mean of 67.96% polymorphism and resolving power (Rp) of 3.41. It is also cleared that radiation is more effective on Sids-1 cultivar with 55.5% polymorphism than on Sakha-93 cultivar with 51.9% polymorphism. Total amplicons found in Sids-1cultivar were 64, ten of them were unique amplicons (UA): 5 UA(+) and 5 UA(−). Irradiation dose (200 Gy) showed the highest number of UA (3 UA− and 3 UA+) in Sids-1cultivar. While, total amplicons found in Sakha-93 cultivar were 58, eight of them were 5 UA(+) and 3 UA(−). Irradiation dose (300 Gy) showed the highest number of UA as 4 UA+. It could be concluded that gamma irradiation of wheat grains produced an appropriate number of generated variations and that ISSR analysis given a useful molecular marker for the symmetry of the mutants.

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Section: Primer Namementioning

confidence: 91%

Physiological and molecular studies on ISSR in two wheat cultivars after exposing to gamma radiation

Aly¹,

Eliwa²,

Maraei³

2019

ScienceAsia

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“…Although the reduced accuracy of estimation of genetic diversity using dominant markers was reported, it has been well documented that ISSR is a reliable technique for detecting DNA polymorphisms and assessing genetic diversity of many woody plant species [16][17][18]. Even though no ISSR analysis study has been carried out on K. latifolia, numerous similar researches have been conducted on other two genera, Rhododendron and Vaccinium, in Ericaceae.…”

Section: Effectiveness Of Issr In K Latifoliamentioning

confidence: 99%

Assessing Genetic Diversity and Population Structure of Kalmia latifolia L. in the Eastern United States: An Essential Step towards Breeding for Adaptability to Southeastern Environmental Conditions

Chappell

Zhang

2020

Sustainability

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Kalmia latifolia L. (mountain laurel), an attractive flowering shrub, is considered to be a high-value ornamental plant for the eastern United States. Limited information on the genetic diversity and structure of K. latifolia is available, which obstructs efficient germplasm utilization and breeding for adaptability to southeastern environmental conditions. In this study, the genetic diversity of 48 wild K. latifolia plants sampled from eight populations in the eastern U.S. was assessed using eight inter simple sequence repeat (ISSR) markers. A total of 116 bands were amplified, 90.52% of which (105) were polymorphic. A high level of genetic diversity at the species level was determined by Nei’s gene diversity (0.3089) and Shannon’s information index (0.4654), indicating that K. latifolia was able to adapt to environmental changes and thus was able to distribute over a wide latitudinal range. In terms of the distribution of genetic diversity, Nei’s genetic differentiation and analysis of molecular variance (AMOVA) showed 38.09% and 29.54% of diversity existed among populations, respectively, elucidating a low-to-moderate level of among-population genetic differentiation. Although a relatively large proportion of diversity was attributed to within-population variation, low diversity within populations (mean genetic diversity within populations (HS) = 0.19) was observed. Both STRUCTURE and unweighted pair group method with arithmetic mean (UPGMA) dendrograms exhibited the clustering of populations that inhabit the same geographic region, and four clusters correlated with four geographic regions, which might be attributed to insect pollination, small population size, and environmental conditions in different habitats. These results function as an essential step towards better conserving and utilizing wild K. latifolia resources, and hence promoting its genetic improvement and breeding for adaptability to southeastern environmental conditions.

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“…Chinese fir (Cunninghamia lanceolata (Lamb) Hook) is one of the major commercial conifer species in China [1,2]. It is a typical subtropical coniferous tree in Southern China [3,4]. Chinese fir is the most important valued timber species due to its fast growth, high yield, and excellent wood quality (i.e., straight and decay resistant) as well as having significant cultural and historical values to the people [3].…”

Section: Introductionmentioning

confidence: 99%

“…Since the 1980s, decreasing production of Chinese fir plantations has been reported, primarily due to soil degradation [7,8]. Currently, the sustainability of Chinese fir plantations is threatened by biodiversity reduction, production loss, soil degradation, and a lack of self-regeneration [4,9]. Therefore, understanding the factors that control regeneration, systematic breeding and selection of the best potential trees for future breeding programs are a major research priority for this tree.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Sucrose, Boric Acid, pH, and Incubation Time on in Vitro Germination of Pollen and Tube Growth of Chinese fir (Cunnighamial lanceolata L.)

Fragallah

Lin

et al. 2019

Forests

Self Cite

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In vitro pollen germination provides a novel approach and strategy to accelerate genetic improvement of tree breeding. Studies about pollen germination and tube growth of Chinese fir are limited. Therefore, this study aimed to investigate the effects of sucrose, boric acid, pH, and time of incubation on pollen germination and tube growth. Pollen from 9 clones were selected. In vitro germination was performed in basic media as control, and in different concentrations of sucrose (0, 10 and 15%), boric acid (0.01, 0.1 and 0.2%), and pH levels (4.5, 5 and 7). Pollen germination rates and tube growth were recorded periodically at 1, 12, 24, and 48 h. The results showed that sucrose imposes significant effects on pollen germination and tube growth. The effects are most obvious at concentration of 15%. Boric acid significantly promoted germination and tube growth. The promotion was most notable in lower concentration of 0.01%. The media adjusted to pH 7.0 boosted the germination and pollen tube growth. The optimum time of incubation was 24 and 48 h for pollen germination and tube growth, respectively. Sucrose, pH, and time of incubation were positively correlated, whereas boric acid negatively correlated with pollen germination and tube growth. This study provided experimental evidences for selecting viable pollens for Chinese fir breeding.

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Genetic diversity and variation of Chinese fir from Fujian province and Taiwan, China, based on ISSR markers

Cited by 17 publications

References 39 publications

Physiological and molecular studies on ISSR in two wheat cultivars after exposing to gamma radiation

Physiological and molecular studies on ISSR in two wheat cultivars after exposing to gamma radiation

Assessing Genetic Diversity and Population Structure of Kalmia latifolia L. in the Eastern United States: An Essential Step towards Breeding for Adaptability to Southeastern Environmental Conditions

Effects of Sucrose, Boric Acid, pH, and Incubation Time on in Vitro Germination of Pollen and Tube Growth of Chinese fir (Cunnighamial lanceolata L.)

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