Effects of Sucrose, Boric Acid, pH, and Incubation Time on in Vitro Germination of Pollen and Tube Growth of Chinese fir (Cunnighamial lanceolata L.)

Fragallah, Seif Aldin Dawina Abdallah; Lin, Shan-Hsiung; Li, Nuo; Ligate, Elly Josephat; Yu, Chang Yeon

doi:10.3390/f10020102

Cited by 29 publications

(14 citation statements)

References 34 publications

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“…They also improve the germination and elongation of pollen tubes by enhancing the calcium effect (Brewbaker and Kwack, 1963). The pH of the in vitro germination medium is an important factor controlling PG and pollen tube development in different plant species (Bellani et al, 1997;Munzuroglu et al, 2003;Burke et al, 2004;Zaman, 2011;Fragallah et al, 2019), because it affects physiological processes through enzyme activation or inhibition (Fan, 2001;Bisswanger, 2014). Finally, the comparatively low percentages of PGM components including enzymes, vitamins, and amino acids can be explained by the fact that pollen grains are rich in these components and therefore do not generally require exogenous supply of such substances in the in vitro PGM (Campos et al, 2008;Komosinska-Vassev et al, 2015).…”

Section: Resultsmentioning

confidence: 99%

A Compendium of in vitro Germination Media for Pollen Research

Tushabe

Rosbakh

2021

Front. Plant Sci.

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The correct choice of in vitro pollen germination media (PGM) is crucial in basic and applied pollen research. However, the methodological gaps (e.g., strong focus of current research on model species and cultivated plants along with the lack of general rules for developing a PGM) makes experimenting with pollen difficult. We closed these gaps by compiling a compendium of optimized in vitro PGM recipes from more than 1800 articles published in English, German, and Russian from 1926 to 2019. The compendium includes 1572 PGM recipes successfully used to germinate pollen grains or produce pollen tubes in 816 species representing 412 genera and 114 families (both monocots and dicots). Among the 110 components recorded from the different PGM recipes, sucrose (89% of species), H3BO3 (77%), Ca2+ (59%), Mg2+ (44%), and K+ (39%) were the most commonly used PGM components. PGM pH was reported in 35% of all studies reviewed. Also, we identified some general rules for creating PGM for various groups of species differing in area of research (wild and cultivated species), phylogenetic relatedness (angiosperms vs. gymnosperms, dicots vs. monocots), pollen physiology (bi- and tri-cellular), biochemistry (starchy vs. starchless pollen grains), and stigma properties (dry vs. wet), and compared the component requirements. Sucrose, calcium, and magnesium concentrations were significantly different across most categories indicating that pollen sensitivity to sugar and mineral requirements in PGM is highly group-specific and should be accounted for when composing new PGM. This compendium is an important data resource on PGM and can facilitate future pollen research.

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Section: Resultsmentioning

confidence: 99%

A Compendium of in vitro Germination Media for Pollen Research

Tushabe

Rosbakh

2021

Front. Plant Sci.

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“…Earlier reports have presented that sucrose concentration affects significantly pollen tube growth (Fragallah et al, 2019).…”

Section: Differential Regulation Of Starch and Sucrose Biosynthesis P...mentioning

confidence: 98%

Investigating the Mechanism of Unilateral Cross Incompatibility in Longan (Dimocarpus longan Lour.) Cultivars (Yiduo × Shixia)

Wang

Huang

et al. 2022

Front. Plant Sci.

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Longan (Dimocarpus longan Lour.) is an important subtropical fruit tree in China. Nearly 90% of longan fruit imports from Thailand are from the cultivar Yiduo. However, we have observed that there exists a unilateral cross incompatibility (UCI) when Yiduo is used as a female parent and Shixia (a famous Chinese cultivar) as a male parent. Here, we performed a comparative transcriptome analysis coupled with microscopy of pistils from two reciprocal pollination combinations [Shixia♂ × Yiduo♀(SY) and Yiduo♀ × Shixia♂(YS)] 4, 8, 12, and 24 h after pollination. We also explored endogenous jasmonic acid (JA) and jasmonyl isoleucine (JA-Ile) levels in pistils of the crosses. The microscopic observations showed that the UCI was sporophytic. The endogenous JA and JA-Ile levels were higher in YS than in SY at the studied time points. We found 7,251 differentially expressed genes from the transcriptome analysis. Our results highlighted that genes associated with JA biosynthesis and signaling, pollen tube growth, cell wall modification, starch and sucrose biosynthesis, and protein processing in endoplasmic reticulum pathways were differentially regulated between SY and YS. We discussed transcriptomic changes in the above-mentioned pathways regarding the observed microscopic and/or endogenous hormone levels. This is the first report on the elaboration of transcriptomic changes in longan reciprocal pollination combination showing UCI. The results presented here will enable the longan breeding community to better understand the mechanisms of UCI.

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“…Boron, in the form of boric acid, plays an essential role in in vitro pollen germination by regulating the pollen tube growth through its ability to stimulate the plasma membrane H + -ATPase (65). Contrasting results were obtained in in vitro pollen germination in Chinese fir (Cunnighamia lanceolata L.) using the same concentration of boric acid, which completely inhibited pollen germination (66). They further stated that not using the optimal concentration of boric acid hinders pollen germination.…”

Section: Effects Of Stages In Flowering In In Vitro Pollen Germinationmentioning

confidence: 99%

Pollen viability and incompatibility in indigenous rice bean (Vigna umbellata (Thunb.) Ohwi & Ohashi)

et al. 2021

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Pollen viability, germination and compatibility are essential in determining the success of pollination and seed setting of high-valued crops. Rice bean (Vigna umbellata (Thunb.) Ohwi & Ohashi) is an underutilized and unexplored indigenous legume with high potential for commercial production. In this study, pollen quality, viability, germination rate and incompatibility among selected six rice bean (V. umbellata) accessions from Barili, Cebu, Philippines were evaluated to determine the barriers and effective pollination habit for increased productivity while retaining the important traits, including high tolerance in poor soils, superior climatic resilience and resistance to pest and diseases. Results of acetocarmine calorimetric assay showed that rice beans’ (V. umbellata) pollens are highly viable, with accessions VU 004 (56.33 ± 4.91%) and VU 007 (54.34 ± 4.53%) having the optimum viability rate. Brewbaker and Kwack medium treated with 0.2 g.l-1 and 0.3 g.l-1 boric acid (H3BO4) enhanced the germination rate in vitro (11.56 ± 5.53% and 9.47 ± 6.50% respectively). Bud (14.96 ± 1.53%) and post-anthesis pollens (10.28 ± 0.94%) have optimum germination rate in 0.2 g.l-1 boric acid media, while anthesis pollens are suitable in media supplemented with sucrose and boric acid alone (12.20 ± 1.50%) and with 0.1 g.l-1 myo-inositol supplementation (8.49 ± 1.86%). Pollination test revealed that rice bean accessions have high self-compatibility (50.76 + 3.45%) and low cross-compatibility (26.57 + 2.49%). The findings provide an important background in understanding the pollen quality and intraspecific interaction among indigenous rice bean (V. umbellata) accessions in Barili, Cebu to improve production and hybridization.

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Effects of Sucrose, Boric Acid, pH, and Incubation Time on in Vitro Germination of Pollen and Tube Growth of Chinese fir (Cunnighamial lanceolata L.)

Cited by 29 publications

References 34 publications

A Compendium of in vitro Germination Media for Pollen Research

A Compendium of in vitro Germination Media for Pollen Research

Investigating the Mechanism of Unilateral Cross Incompatibility in Longan (Dimocarpus longan Lour.) Cultivars (Yiduo × Shixia)

Pollen viability and incompatibility in indigenous rice bean (Vigna umbellata (Thunb.) Ohwi & Ohashi)

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