Genetic diversity and population structure of wild Dipsacus asperoides in China as indicated by ISSR markers

Chen, D.X.; Li, L.Y.; Zhang, X.; Wang, Y.; Zhang, Z.

doi:10.4238/2014.february.14.12

Cited by 3 publications

(3 citation statements)

References 11 publications

(10 reference statements)

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“…Saponins, the major bioactive compound in D. asperoides , are isolated primarily from the taproots and widely used to treat fractures (Zhang et al 2003; Jung et al 2012). D. asperoides in the wild has diminished as a result of exploitation (Zhang et al 1997; Chen et al 2014; Wang et al 2016), and the cultivated plant contains lower levels of dipsacus saponin VI than the plant in the wild. Therefore, rapid, efficient and environmentally sustainable methods are needed to obtain this and other saponins from D. asperoides (Cira et al 2008; Jiao et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Association between dipsacus saponin VI level and diversity of endophytic fungi in roots of Dipsacus asperoides

Gong

Zhou

Xiao

et al. 2019

World J Microbiol Biotechnol

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Dipsacus asperoides contains multiple pharmacologically active compounds. The principal are saponins. The plant can be cultivated, but it contains lower levels of bioactive compounds than the plant in the wild. It may be the reason to exploit the endophytic fungi that colonize the plant roots in order to produce bioactive compounds. However, the endophytic fungi of D. asperoides have not been analyzed in detail. In this study, we isolated and identified 46 endophytic fungal strains from the taproots, lateral roots and leaves, and we used morphological and molecular biological methods to assign them into 15 genera: Fusarium sp., Ceratobasidium sp., Chaetomium sp., Penicillium sp., Aspergillus sp., Talaromyces sp., Cladosporium sp., Bionectria sp., Mucor sp., Trichoderma sp., Myrothecium sp., Clonostachys sp., Ijuhya sp., Leptosphaeria sp. and Phoma sp. Taproots contained abundant endophytic fungi, the numbers of which correlated positively with level of dipsacus saponin VI. Primary fermentation of several endophytic fungal strains from taproots showed that Fusarium, Leptosphaeria, Ceratobasidium sp. and Phoma sp. can produce the triterpenoid saponin. These results may guide efforts to sustainably produce bioactive compounds from D. asperoides . Graphical abstract Electronic supplementary material The online version of this article (10.1007/s11274-019-2616-y) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Association between dipsacus saponin VI level and diversity of endophytic fungi in roots of Dipsacus asperoides

Gong

Zhou

Xiao

et al. 2019

World J Microbiol Biotechnol

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“…The oldest report on Dipsacus genus can be found in Shen Nong's Herbal Classic (Shen Nong Ben Cao Jing in Chinese) [6,11,18]. D. asper is widespread in the southern and northern regions of China such as the Hunan, Yunnan, Gansu, and Shanxi Provinces [12,19]. The growing demand for this species and its mass harvesting has significantly weakened its population in a natural state [19,60].…”

Section: Dipsacus Sppmentioning

confidence: 99%

“…D. asper is widespread in the southern and northern regions of China such as the Hunan, Yunnan, Gansu, and Shanxi Provinces [12,19]. The growing demand for this species and its mass harvesting has significantly weakened its population in a natural state [19,60]. D. asperoides is cultivated on a large scale, mainly in Hefeng City, Hubei Province.…”

Section: Dipsacus Sppmentioning

confidence: 99%

Dipsacus and Scabiosa Species—The Source of Specialized Metabolites with High Biological Relevance: A Review

Skała

Szopa

2023

Molecules

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The genera Dipsacus L. and Scabiosa L. of the Caprifoliaceae family are widely distributed in Europe, Asia, and Africa. This work reviews the available literature on the phytochemical profiles, ethnomedicinal uses, and biological activities of the most popular species. These plants are rich sources of many valuable specialized metabolites with beneficial medicinal properties, such as triterpenoid derivatives, iridoids, phenolic acids, and flavonoids. They are also sources of essential oils. The genus Dipsacus has been used for centuries in Chinese and Korean folk medicines to treat bone (osteoporosis) and joint problems (rheumatic arthritis). The Korean Herbal Pharmacopoeia and Chinese Pharmacopoeia include Dipsaci radix, the dried roots of D. asperoides C.Y.Cheng & T.M.Ai. In addition, S. comosa Fisch. ex Roem & Schult. and S. tschiliiensis Grunning are used in traditional Mongolian medicine to treat liver diseases. The current scientific literature data indicate that these plants and their constituents have various biological properties, including inter alia antiarthritic, anti-neurodegenerative, anti-inflammatory, antioxidant, anticancer, and antimicrobial activities; they have also been found to strengthen tendon and bone tissue and protect the liver, heart, and kidney. The essential oils possess antibacterial, antifungal, and insecticidal properties. This paper reviews the key biological values of Dipsacus and Scabiosa species, as identified by in vitro and in vivo studies, and presents their potential pharmacological applications.

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Genetic structure of the Ukrainian water buffalo population by ISSR-PCR markers

Mokhnachova¹

2022

Bìol. Tvarin

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The study of intraspecific genetic diversity of cattle, including species of the genus Bubalus from the subfamily Bull, is important because of the reduction of biodiversity of farm animals. The basis of genetic diversity is its genetic component. The loss of native species and breeds of cattle is a real threat to the biosphere, as the resilience of natural ecosystems and agroecosystems is directly linked to their genetic ability to adapt to environmental conditions. Polymorphism of ISSR-markers of the Ukrainian population of water buffaloes (Bubalus bubalis) from the farm of “TASBIO” LLC (Chernihiv region) was analyzed in 66 animals selected for the study. Genomic DNA samples were isolated from venous blood with a standard set of reagents. Genotyping was performed using specific ISSR primers: (ACC)6G, (GAG)6C, (AG)9C, (CTC)6C, (AG)8CA, (AG)8CGand (GA)6CC. We determined the difference in spectra both by the number of amplicons, their lengths (number of nucleotides) and by their polymorphism. As a result of the study, all primers showed polymorphism of buffalo DNA regions. Amplicons were defined in the range from 200 bp up to 4000 bp. Analysis of ISSR spectra revealed 87 loci, of which 71 were polymorphic. (AG)8CA-marker was the least polymorphic (PIC=0.234), (CTC)6C the most polymorphic (PIC=0.389). Conservative loci were found in four ISSR markers: 6 in (AG)8CA marker, 5 in (AG)8CG, 4 in (GA)6CC, and 1 in (AG)9C. 67 species-specific loci were identified for the Ukrainian water buffalo population: 10 for (AG)9C, 3 loci for (ACC)6G, 4 for (GAG)6C, 7 for (CTC)6C, 15 for (AG)8CA, and 14 in (AG)8CG and (GA)6CC. The ISSR primers used are recommended for molecular genetic analysis of buffalo DNA polymorphism.

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Genetic diversity and population structure of wild Dipsacus asperoides in China as indicated by ISSR markers

Cited by 3 publications

References 11 publications

Association between dipsacus saponin VI level and diversity of endophytic fungi in roots of Dipsacus asperoides

Association between dipsacus saponin VI level and diversity of endophytic fungi in roots of Dipsacus asperoides

Dipsacus and Scabiosa Species—The Source of Specialized Metabolites with High Biological Relevance: A Review

Genetic structure of the Ukrainian water buffalo population by ISSR-PCR markers

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