Association between dipsacus saponin VI level and diversity of endophytic fungi in roots of Dipsacus asperoides

Abstract

Dipsacus asperoides contains multiple pharmacologically active compounds. The principal are saponins. The plant can be cultivated, but it contains lower levels of bioactive compounds than the plant in the wild. It may be the reason to exploit the endophytic fungi that colonize the plant roots in order to produce bioactive compounds. However, the endophytic fungi of D. asperoides have not been analyzed in detail. In this study, we isolated and identified 46 endophytic fungal strains from the taproots, lateral roots and leaves, and we used morphological and molecular biological methods to assign them into 15 genera: Fusarium sp., Ceratobasidium sp., Chaetomium sp., Penicillium sp., Aspergillus sp., Talaromyces sp., Cladosporium sp., Bionectria sp., Mucor sp., Trichoderma sp., Myrothecium sp., Clonostachys sp., Ijuhya sp., Leptosphaeria sp. and Phoma sp. Taproots contained abundant endophytic fungi, the numbers of which correlated positively with level of dipsacus saponin VI. Primary fermentation of several endophytic fungal strains from taproots showed that Fusarium, Leptosphaeria, Ceratobasidium sp. and Phoma sp. can produce the triterpenoid saponin. These results may guide efforts to sustainably produce bioactive compounds from D. asperoides . Graphical abstract Electronic supplementary material The online version of this article (10.1007/s11274-019-2616-y) contains supplementary material, which is available to authorized users.

Isolation, diversity, and antimicrobial activity of fungal endophytes from Rohdea chinensis (Baker) N.Tanaka (synonym Tupistra chinensis Baker) of Qinling Mountains, China

“…Glomerellaceae (RA, 29.92%), Nectriaceae (RA, 15.90%), Aspergillaceae (RA, 12.94%) were three most abundant families in this study as shown in Fig. 1C In previous studies, Colletotrichum, Fusarium, Aspergillus were the dominant genera of endophytes in different host plants (Tan et al, 2018;Gong et al, 2019;Salazar-Cerezo et al, 2018) and similar result was obtained in this study (Colletotrichum (IF, 29.92%), Fusarium (IF, 8.36%), Aspergillus (IF, 8.09%)). In addition, fungi representing the genera Biscogniauxia, Leptosphaeria, Leptostroma, Annulohypoxylon, Stachybotrys, Didymella, Peniophora, Dactylonectria, Nectria, Peniophora, Rhodotorula, Setophaeosphaeria, Clitopilus were obtained in this study.…”

“…In addition, fungi representing the genera Biscogniauxia, Leptosphaeria, Leptostroma, Annulohypoxylon, Stachybotrys, Didymella, Peniophora, Dactylonectria, Nectria, Peniophora, Rhodotorula, Setophaeosphaeria, Clitopilus were obtained in this study. Endophytes in these genera have been reported much less frequently in previous studies (Sritharan et al, 2019;Gong et al, 2019;Li et al, 2018).…”

“…In addition, endophytic fungi that inhabit the interiors various plant tissues without causing disease (Petrini, 1991) have been increasingly identified as a source of novel metabolites. Medicinal plants harbor a great diversity of culturable endophytes (Tan et al, 2014;Tan et al, 2018;Gong et al, 2019), which have can produce structurally fascinating and biologically active secondary metabolites (Helaly, Thongbai & Stadler, 2018;Kumari et al, 2018;Leylaie & Zafari, 2018;Mousa et al, 2015).…”

Illumina-based analysis yields new insights into the diversity and composition of endophytic fungi in cultivated Huperzia serrata

“…In addition, some of endophytic fungi in H. serrata can produce huperzine A [15][16][17]. The distribution of some endophytic fungi in the host plants exhibited tissue specificity, which is an important influencing factor for accumulation of bioactive substances in different tissues [18,19]. Although there are many studies focusing on endophytes of H. serrata, the understanding of the endophytic community in different tissues associated with H. serrata is still limited.…”

“…serrata can produce huperzine A [ 15 – 17 ]. The distribution of some endophytic fungi in the host plants exhibited tissue specificity, which is an important influencing factor for accumulation of bioactive substances in different tissues [ 18 , 19 ]. Although there are many studies focusing on endophytes of H .…”