ABSTRACT. In order to evaluate the genetic diversity and genetic structure of wild Dipsacus asperoides, we surveyed genetic polymorphisms in 288 individuals from 12 populations using ISSR. A total of 240 bands were amplified, among which 190 were polymorphic loci. At the species level, genetic diversity was found to be abundant: PPB = 79.17%, N E = 1.2152, H = 0.1361, and H sp = 0.2213. At the population level, genetic diversity was lower: PPB = 30.76 %, N E = 1.0786, H = 0.0897, and H pop = 0.1375. The calculated Nei genetic differentiation coefficient was 0.3406, which is consistent with the calculated Shannon population genetic differentiation coefficient of 0.3787. This is commonly taken to indicate that most of the genetic variation existed within the populations. Gene flow was calculated as N m = 0.9679, suggesting that gene exchange only occurred at a low level. Based on the Nei genetic distance, the 12 populations were divided into 4 categories. Our results suggest that D. asperoides has 6341 Genetic diversity in wild Dipsacus asperoides ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 13 (3): 6340-6349 (2014) abundant genetic diversity and provides a foundation for the protection and improvement of germplasm resources.
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