Genetic diversity and distribution differ between long-established and recently introduced populations in the invasive mosquito Aedes albopictus

Sherpa, Stéphanie; Rioux, Delphine; Pougnet-Lagarde, Charlotte; Després, Laurence

doi:10.1016/j.meegid.2017.12.018

Cited by 34 publications

(41 citation statements)

References 78 publications

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“…This pattern supports that natural dispersal occurs at the local scale and reveals that geographically close populations are close relatives (<2 km). This result can be explained by the reproductive biology of A. albopictus that leads to inbred progeny because adults mate soon after emerging from small breeding site, which is supported by our estimations of local inbreeding (positive F IS in Table S5), as also found in many other studies (Beebe et al., 2013; Delatte et al., 2013; Kamgang et al., 2011; Manni et al., 2015; Sherpa, Rioux, Pougnet‐Lagarde, & Després, 2018).…”

Section: Discussionsupporting

confidence: 89%

Landscape does matter: Disentangling founder effects from natural and human‐aided post‐introduction dispersal during an ongoing biological invasion

Sherpa

Renaud

Guéguen

et al. 2020

Journal of Animal Ecology

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Environmental features impacting the spread of invasive species after introduction can be assessed using population genetic structure as a quantitative estimation of effective dispersal at the landscape scale. However, in the case of an ongoing biological invasion, deciphering whether genetic structure represents landscape connectivity or founder effects is particularly challenging. We examined the modes of dispersal (natural and human‐aided) and the factors (landscape or founders history) shaping genetic structure in range edge invasive populations of the Asian tiger mosquito, Aedes albopictus, in the region of Grenoble (Southeast France). Based on detailed occupancy–detection data and environmental variables (climatic, topographic and land‐cover), we modelled A. albopictus potential suitable area and its expansion history since first introduction. The relative role of dispersal modes was estimated using biological dispersal capabilities and landscape genetics approaches using genome‐wide SNP dataset. We demonstrate that both natural and human‐aided dispersal have promoted the expansion of populations. Populations in diffuse urban areas, representing highly suitable habitat for A. albopictus, tend to disperse less, while roads facilitate long‐distance dispersal. Yet, demographic bottlenecks during introduction played a major role in shaping the genetic variability of these range edge populations. The present study is one of the few investigating the role of founder effects and ongoing expansion processes in shaping spatial patterns of genetic variation in an invasive species at the landscape scale. The combination of several dispersal modes and large proportions of continuous suitable habitats for A. albopictus promoted range filling of almost its entire potential distribution in the region of Grenoble only few years after introduction.

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Section: Discussionsupporting

confidence: 89%

Landscape does matter: Disentangling founder effects from natural and human‐aided post‐introduction dispersal during an ongoing biological invasion

Sherpa

Renaud

Guéguen

et al. 2020

Journal of Animal Ecology

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“…To estimate the influence of the m and M values on RAD tag fragment recovery, we determined the number of reconstructed fragments, their mean coverage and the proportion of polymorphic fragments for each value of M and m tested. We also evaluated the impact of these parameters on population genetics results by performing, for all m and M values, some of the most commonly used analyses using ddRADseq data (Capblancq et al, 2015; Kjeldsen et al, 2016; Black et al, 2017; Nunziata et al, 2017; Settepani et al, 2017; Elleouet & Aitken, 2018; Sherpa, Rioux, Pougnet-Lagarde, et al, 2018), i.e. mean individual heterozygosity, F ST among populations (estimated with the adegenet R package (Jombart, 2008)), Principal Component Analysis (PCA, using the adegenet R package (Jombart, 2008)), genetic structure with sNMF (using the LEA R package (Frichot & François, 2015)) and evolutionary history reconstruction using Approximate Bayesian Computation (performed with the diyABC program (Cornuet et al, 2014)).…”

Section: Methodsmentioning

confidence: 99%

“…Among these, double-digest RADseq, or ddRADseq (Peterson et al, 2012), is highly customizable as regards the final number of loci, depending on the choice of enzymes and range of fragment size selected. The ddRADseq approach has been applied with success to many purposes including population genetic studies (Kjeldsen et al, 2016; Black, Seears, Hollenbeck, & Samollow, 2017; Sherpa, Rioux, Goindin, et al, 2018), phylogenetic reconstructions (DaCosta & Sorenson, 2016; Vargas, Ortiz, & Simpson, 2017; Boubli et al, 2018; Lee et al, 2018; Sherpa, Rioux, Pougnet-Lagarde, & Després, 2018), demographic inferences (Capblancq, Després, Rioux, & Mavárez, 2015; Nunziata, Lance, Scott, Lemmon, & Weisrock, 2017; Settepani et al, 2017; Elleouet & Aitken, 2018) and landscape genetic analyses (Saenz-Agudelo et al, 2015; Johnson, Gaddis, Cairns, Konganti, & Krutovsky, 2017). Despite the recognized advantages of the ddRADseq technique, several limitations and weaknesses arose in the literature (Davey et al, 2013; K. R. Andrews et al, 2016; Lowry et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Double-digest RAD-sequencing: do wet and dry protocol parameters impact biological results?

Cumer

Pouchon

Boyer

et al. 2018

Preprint

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1 2 1 3 Running headline: 1 4 Impact of wet and dry protocol of ddRAD-seq 1 5 1 6 2 ABSTRACT 1 7 1 81. Next-generation sequencing technologies have opened a new era of research in genomics. 9Among these, restriction enzyme-based techniques such as restriction-site associated DNA 2 0 sequencing (RADseq) or double-digest RAD-sequencing (ddRADseq) are now widely used in 2 1 many population genomics fields. From DNA sampling to SNP calling, both wet and dry 2 2 protocols have been discussed in the literature to identify key parameters for an optimal loci 2 3 reconstruction. 2 4 2. The impact of these parameters on downstream analyses and biological results drawn from 2 5RADseq or ddRADseq data has however not been fully explored yet. In this study, we tackled 2 6 this issue by investigating the effects of ddRADseq laboratory (i.e. wet protocol) and 2 7 bioinformatics (i.e. dry protocol) settings on loci reconstruction and inferred biological signal 2 8 at two evolutionary scale using two systems: a complex of butterfly species (Coenonympha 2 9 sp.) and populations of Common beech (Fagus sylvatica). 3 0 3. Results suggest an impact of wet protocol parameters (DNA quantity, number of PCR 3 1 cycles during library preparation) on the number of recovered reads and SNPs, the number of 3 2unique alleles and individual heterozygosity. We also found that bioinformatic settings (i.e. 3 3 clustering and minimum coverage thresholds) impact loci reconstruction (e.g. number of loci, 3 4mean coverage) and SNP calling (e.g. number of SNPs, heterozygosity). We however do not 3 5 detect an impact of parameter settings on three types of analysis performed with ddRADseq 3 6 data: measure of genetic differentiation, estimation of individual admixture, and demographic 3 7inferences. In addition, our work demonstrates the high reproducibility and low rate of 3 8

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“…We reanalyzed previously published genomic data obtained from double-digest restriction-associated DNA sequencing (ddRADseq; Sherpa, Blum, Capblancq, et al, 2019;Sherpa, Rioux, Pougnet-Lagarde, & Després, 2018). Sequences of 110 bp for 90 European populations (N = 551) were mapped to the Ae.…”

Section: Genetic Data Acquisitionmentioning

confidence: 99%

Predicting the success of an invader: Niche shift versus niche conservatism

Sherpa

Guéguen

Renaud

et al. 2019

Ecology and Evolution

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Invasive species can encounter environments different from their source populations, which may trigger rapid adaptive changes after introduction (niche shift hypothesis). To test this hypothesis, we investigated whether postintroduction evolution is correlated with contrasting environmental conditions between the European invasive and source ranges in the Asian tiger mosquito Aedes albopictus. The comparison of environmental niches occupied in European and source population ranges revealed more than 96% overlap between invasive and source niches, supporting niche conservatism. However, we found evidence for postintroduction genetic evolution by reanalyzing a published ddRADseq genomic dataset from 90 European invasive populations using genotype–environment association (GEA) methods and generalized dissimilarity modeling (GDM). Three loci, among which a putative heat‐shock protein, exhibited significant allelic turnover along the gradient of winter precipitation that could be associated with ongoing range expansion. Wing morphometric traits weakly correlated with environmental gradients within Europe, but wing size differed between invasive and source populations located in different climatic areas. Niche similarities between source and invasive ranges might have facilitated the establishment of populations. Nonetheless, we found evidence for environmental‐induced adaptive changes after introduction. The ability to rapidly evolve observed in invasive populations (genetic shift) together with a large proportion of unfilled potential suitable areas (80%) pave the way to further spread of Ae. albopictus in Europe.

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Genetic diversity and distribution differ between long-established and recently introduced populations in the invasive mosquito Aedes albopictus

Cited by 34 publications

References 78 publications

Landscape does matter: Disentangling founder effects from natural and human‐aided post‐introduction dispersal during an ongoing biological invasion

Landscape does matter: Disentangling founder effects from natural and human‐aided post‐introduction dispersal during an ongoing biological invasion

Double-digest RAD-sequencing: do wet and dry protocol parameters impact biological results?

Predicting the success of an invader: Niche shift versus niche conservatism

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