Genetic diversity and assessment of 23 microsatellite markers for parentage testing of Santa Inês hair sheep in Brazil

Souza, Carla A.; Paiva, Samuel Rezende; McManus, Concepta; Azevedo, H. C.; Mariante, A. da S.; Grattapaglia, Dário

doi:10.4238/2012.may.8.4

Cited by 24 publications

(20 citation statements)

References 25 publications

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“…The majority of the markers were highly polymorphic and generally in Hardy-Weinberg equilibrium, except for the marker DU223896 that showed the largest difference between observed and expected heterozygosity, and DU206192 for the highest null alleles frequency. The average number of alleles per marker (11.21) was considerably higher than that found in sheep populations in Bhutan (Dorji et al, 2010) and in Iran (Saberivand et al, 2011), whereas similar results were reported by Souza et al (2012) in Santa Inês sheep breed. Glowatzki-Mullis et al (2007) analyzed 10 different breeds with a 19-microsatellite multiplex (of which 10 are in common with our study) and found an average maximum of 7.79 alleles for Red Engadin sheep.…”

Section: Discussionmentioning

confidence: 39%

Parentage verification of Valle del Belice dairy sheep using multiplex microsatellite panel

Rosa

Sardina

Mastrangelo

et al. 2013

Small Ruminant Research

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Section: Discussionmentioning

confidence: 39%

Parentage verification of Valle del Belice dairy sheep using multiplex microsatellite panel

Rosa

Sardina

Mastrangelo

et al. 2013

Small Ruminant Research

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“…Lowest heterozygosity and greatest inbreeding coefficient (F IS ) values exhibited by CRO population are shown in bold font. In general, the analyzed populations showed reasonably high allelic richness and heterozygosity values compared to some African (Soma et al, 2012;Kunene et al, 2014;Mukhongo et al, 2014), European (Salamon et al, 2014), Asian (Al-Barzinji et al, 2011;Blackburn et al, 2011b), and American (Blackburn, et al, 2011a;Souza et al, 2012;Ferreira et al, 2014) sheep breeds. The lowest values of AR and heterozygosity were observed in the crossbred population in which H O was notably lower than the H E (Table 2).…”

Section: Resultsmentioning

confidence: 99%

Contribution of microsatellites markers in the clarification of the origin, genetic risk factors, and implications for conservation of Tunisian native sheep breeds

et al. 2016

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ABSTRACT. The genetic diversity and genetic relationship of the two main groups of African sheep, thin-tailed and fat-tailed sheep, represented by the indigenous Tunisian sheep breeds "Barbarine" (BAR, fat-tailed) and "Queue Fine de l'Ouest" (QFO, thin-tailed) were investigated. The genotypes of 110 animals belonging to these two breeds and their crossbreed (CRO) were assessed using 17 microsatellite markers. The results showed high levels of genetic diversity and a total of 256 alleles were identified in the whole population. The mean values of observed and expected heterozygosity were 0.719 and 0.789, respectively, and the mean allelic richness estimate was 10.89. values over all loci indicated a notable level of inbreeding within the whole population. However, the F ST value (0.007) showed a low level of genetic differentiation between these two native breeds. The high level of both gene flow and molecular coancestry coefficient detected between the two breeds and their CRO revealed an old miscegenation between the BAR and QFO breeds. The clustering analysis performed with the STRUCTURE software confirmed gene flow between these two breeds. Results arising from this study provide evidence regarding the genetic structure and variability of the two main local sheep breeds, and the implications of their actual management, which indicates the need for an urgent conservation strategy in order to prevent significant gene flow and preserve the remaining breed specificity for future generations.

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“…PCR were performed using a commercially available panel of eight microsatellite loci as previously described by Souza et al (2012) (CSRD247, HSC, OarAE129, MAF214, OarFCB304, OarCP49, SPS113, and D5S2). The reactions were performed using a multiplex fluorescent system, with the eight markers included in the same reaction.…”

Section: Microsatellite Locimentioning

confidence: 99%

Genetic diversity of locally adapted sheep from Pantanal region of Mato Grosso do Sul

Crispim¹,

Grisolia²,

Seno³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Sheep of the Pantaneiro breed and seven other breeds, raised in the State of Mato Grosso do Sul, Brazil, were genotyped using eight microsatellite loci. The aim of the present study was to determine the genetic variability, phylogenetic relationship, and patterns of gene introgression and miscegenation among the animals surveyed, to obtain information about the genetic structure of locally adapted sheep in Mato Grosso do Sul. A total of 195 animals were used for genetic analysis. The Pantaneiro breed had the largest average number of alleles/locus (9.25), and higher allelic richness (6.95), while the Dorper population had the lowest values for these parameters (4.88 and 3.86, respectively). Analysis of genetic distance values and genetic structure between populations made it possible to characterize these animals with regard to distinct genetic groups. Average expected heterozygosity ranged from 0.72 (Pantaneiro) to 0.55 (Dorper), while average observed heterozygosity ranged from 0.63 (White Dorper) to 0.54 (Dorper). On the basis of the statistical parameters evaluated, it 5459 ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 12 (4): 5458-5466 (2013) Genetic diversity in Pantaneiro sheep was possible to demonstrate that when compared to other populations, the Pantaneiro breed represented a reservoir of genetic diversity with rare and useful alleles for genetic improvement, emphasizing the importance of preserving the breed.

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Genetic diversity and assessment of 23 microsatellite markers for parentage testing of Santa Inês hair sheep in Brazil

Cited by 24 publications

References 25 publications

Parentage verification of Valle del Belice dairy sheep using multiplex microsatellite panel

Parentage verification of Valle del Belice dairy sheep using multiplex microsatellite panel

Contribution of microsatellites markers in the clarification of the origin, genetic risk factors, and implications for conservation of Tunisian native sheep breeds

Genetic diversity of locally adapted sheep from Pantanal region of Mato Grosso do Sul

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