Genetic diversity analysis of Capparis spinosa L. populations by using ISSR markers

C, Liu; Gong, Xue; B, Cheng; X, Wang; He, Jun; Gh, Liu; Yang, Wenting

doi:10.4238/2015.december.9.19

Cited by 14 publications

(11 citation statements)

References 18 publications

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“…Subcluster 1 of Cluster I has only The three clusters resolved by STRUCTURE are consistent with those inferred in the dendrogram. Using the same type of molecular marker, Liu et al (2015) observed similar results, the formation of three groups in C. spinosa. Accession VCUR-802 from Itabaiana/ SE shows some affinity to each of the three groups in the STRUCTURE plot, suggesting admixture.…”

Section: Discussionsupporting

confidence: 66%

“…Because they are dominant loci, it is not possible to distinguish heterozygotes from homozygotes; however, multiple loci can be produced from each amplification with polymerase chain reaction (PCR; Goulão and Oliveira, 2001). Several genetic diversity studies on medicinal plants, both in natural populations and in collections of germplasm banks, have been carried out using ISSRs, such as studies on Capparis spinosa, Eucommia ulmoides, and Pogostemon cablin (Liu et al, 2015;Yu et al, 2015;Sandes et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity analysis of Varronia curassavica Jacq. accessions using ISSR markers

et al. 2016

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ABSTRACT. Varronia curassavica Jacq. is a medicinal and aromatic plant from Brazil with significant economic importance. Studies on genetic diversity in active germplasm banks (AGB) are essential for conservation and breeding programs. The aim of this study was to analyze the genetic diversity of V. curassavica accessions of the AGB of Medicinal and Aromatic Plants of the Federal University of Sergipe (UFS), using inter-simple sequence repeat molecular markers. Twentyfour primers were tested, and 14 were polymorphic and informative, resulting in 149 bands with 97.98% polymorphism. The UPGMA dendrogram divided the accessions into Clusters I and II. Jaccard similarity coefficients for pair-wise comparisons of accessions ranged between 0.24 and 0.78. The pairs of accessions VCUR-001/VCUR-503, VCUR-001/VCUR-504, and VCUR-104/VCUR-501 showed relatively low similarity (0.24), and the pair of accessions VCUR-402/VCUR-403 showed medium similarity (0.78). Twenty-eight accessions were divided into three distinct clusters, according to the STRUCTURE analysis. The genetic diversity of V. curassavica in the AGB of UFS is low to medium, and it requires expansion. Accession VCUR-802 is the most suitable for selection in breeding program of this species, since it clearly represents all of the diversity present in the AGB.

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Section: Discussionsupporting

confidence: 66%

Section: Introductionmentioning

confidence: 99%

Genetic diversity analysis of Varronia curassavica Jacq. accessions using ISSR markers

et al. 2016

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“…(Brito et al, 2016), in Capparis spinosa L. (Liu et al, 2015), in Rheum (Tabin et al, 2016), in Withania somnifera (Khan and Shah, 2016), and in Panax stipuleanatus Tsai (Trieu et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of native populations of Croton tetradenius Baill. using ISSR markers

Almeida-Pereira¹,

Silva²,

Alves³

et al. 2017

Genet. Mol. Res.

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ABSTRACT. Brazil has about 300 Croton species in different types of vegetation. Croton tetradenius Baill., which is endemic to the Northeast region and predominant in the Caatinga vegetation, stands out among the several species of this genus. Considering the importance of knowing the genetic variability of a species, the objective of this study was to analyze the genetic diversity of the genotypes of natural populations of C. tetradenius in the State of Sergipe, using ISSR molecular markers. Forty individuals were sampled in four natural populations of the State of Sergipe, Brazil. Thirteen primers were used for DNA amplification using ISSR-PCR, totaling 77 amplified fragments, of which 94.8% were polymorphic. Results of the cluster analysis obtained by the Jaccard's similarity index, using the UPGMA method, resulted in the formation of six distinct clusters. Analysis of molecular variance (AMOVA), used to estimate the genetic variability among populations, revealed significant genetic variance (P < 0.01) between and within the 2 C.S. Almeida-Pereira et al.Genetics and Molecular Research 16 (2): gmr16029602 studied populations, and most of the genetic diversity was found (87%) within populations. According to the Jaccard's similarity index, none of the studied plants was genetically identical. CTE210 and CTE305 presented high similarity index (0.76), while CTE105 presented low similarity index (<0.16) with all related individuals. ISSR markers were efficient and allowed the formation of a molecular profile, and had sufficient polymorphism to estimate the genetic variability between the accessions of the studied populations.

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“…Using random-amplified polymorphic DNA (RAPD) and isoenzyme approaches, Wang et al (2008) reported the low diversity of T. sinensis natural resources in China. Compared with RAPD, ISSR and SRAP have higher reproducibility and are more effective for genetic diversity analyses and germplasm evaluation (Shao et al, 2010;Medri et al, 2011;Niu et al, 2015;Liu et al, 2008Liu et al, , 2015Souza-Sobreira et al, 2015). Using these two marker systems, we studied the genetic diversity and genetic relationships of five T. sinensis populations (100 individuals/sample) representing different climates and geographical locations in China.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of Toona sinensis Roem in China revealed by ISSR and SRAP markers

et al. 2016

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ABSTRACT. Toona sinensis Roem has an important value as a type of traditional vegetable and Chinese medicinal herb, and is also a valuable source of wood in China. In this study, we used the inter-simple sequence repeat (ISSR) and sequence-related amplified polymorphism (SRAP) markers to assess the level and pattern of genetic diversity in five domesticated T. sinensis populations in China. Our results indicated a relatively low level of genetic diversity both at species (Hs = 0.1662, 0.2098, respectively) and population levels (Hs = 0.0978, 0.1145, respectively). Molecular variance analyses revealed a relatively high degree of differentiation among populations (G ST = 0.3901, 0.4498), and low levels of gene flow (N m = 0.7816 and 0.6116). We divided the five populations into two groups by cluster analysis: group one consists of populations collected from the south part of China (e.g., Yuxi, Yunan Province and Zuanjiang, Chongqing Municipality), and group two contains those cultivated in north part of China (e.g., Hengshui, Hebei Province, Jinan and Rizhao, Shandong Province). The correlation of genetic relationships among populations fits well with their geographical distribution (Mantel test; r = 0.7236 and 0.6789, respectively). Asexual propagation, limited gene flow and geographic isolation are most likely the key factors associated with the observed genetic structure of T. sinensis grown in China. The present study indicated that both ISSR and SRAP markers were effective and reliable for assessing the degree of T. sinensis genetic variations.

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Genetic diversity analysis of Capparis spinosa L. populations by using ISSR markers

Cited by 14 publications

References 18 publications

Genetic diversity analysis of Varronia curassavica Jacq. accessions using ISSR markers

Genetic diversity analysis of Varronia curassavica Jacq. accessions using ISSR markers

Genetic diversity of native populations of Croton tetradenius Baill. using ISSR markers

Genetic diversity of Toona sinensis Roem in China revealed by ISSR and SRAP markers

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