Genetic diversity among snake melon landraces (<i>Cucumis Melo</i> Var. <i>Flexuosus</i>) using molecular descriptors

Akash, Muhanad; Awad, N. G. H.; Kasrawi, M. A.

doi:10.1080/11263504.2019.1587536

Cited by 8 publications

(7 citation statements)

References 24 publications

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“…triuncialis accessions [ 50 ]. Mismatching results among the dendrograms being generated by different markers in different plants were indicated, such as in snake melon [ 51 ], sponge gourd [ 52 ], and bamboos [ 53 ]. The results of PCoA plots for the three marker systems confirmed the results of cluster analyses.…”

Section: Discussionmentioning

confidence: 99%

Assessment of genetic diversity among Iranian Aegilops triuncialis accessions using ISSR, SCoT, and CBDP markers

Khodaee

Azizinezhad

Etminan

et al. 2021

Journal of Genetic Engineering and Biotechnology

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Background Crop wild relatives (CWRs) are commonly used as a suitable genetic reservoir for plant breeding. They can be used for enhancing the tolerance of plant varieties to biotic and abiotic stresses. Studying the genetic diversity of related wheat species in Iran could be useful to improve different traits of bread wheat, since the country is one of the major centers of genetic diversity and distribution of Aegilops species. Therefore, the aim of the present study was to determine the relationship among 48 Aegilops triuncialis accessions using three DNA marker systems, including start codon targeted (SCoT), CAAT box-derived polymorphism (CBDP), and inter-simple sequence repeat (ISSR) markers. Results A total of 359 amplified DNA fragments were generated using 13 CBDP, 14 SCoT, and 16 ISSR primers that produced 96, 147, and 152 bands, respectively. The discriminating power of the three markers was assessed using polymorphism information content (PIC), marker index (MI), and resolving power (Rp). The mean values of PIC for ISSR, SCoT, and CBDP markers were 0.3, 0.26, and 0.34, respectively, indicating the efficiency of the three markers in detecting polymorphism among the studied accessions. ISSR markers had the highest values of MI, Rp, and polymorphism percentage as compared to SCoT and CBDP markers. Based on the Shannon index and heterozygosity values, genetic diversity in the Alborz population was more than in other populations. The accessions were classified into six, five, and five groups based on ISSR, SCoT, and CBDP using the UPGMA method. According to the results of cluster and PCoA analyses, the variation patterns corresponded with the geographical distribution of the Ae. triuncialis accessions. Conclusions The three markers provided a comprehensive pattern of the genetic diversity among the Iranian Ae. triuncialis accessions. This information could allow for a future insight into wheat breeding programs.

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Section: Discussionmentioning

confidence: 99%

Assessment of genetic diversity among Iranian Aegilops triuncialis accessions using ISSR, SCoT, and CBDP markers

Khodaee

Azizinezhad

Etminan

et al. 2021

Journal of Genetic Engineering and Biotechnology

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“…Nine ISSR primers that produced 71 monomorphic bands between Palestinian melon accessions were used by Mallah (2014). While the analysis carried out by Akash et al (2020). The 14 ISSR primers developed 63 bands, among which 27 (43 per cent) were polymorphic, and divided the landraces into two classes ranging from a high among 0.97 to a low of 0.39 per cent similarity.…”

Section: Resultsmentioning

confidence: 99%

The Determination of the Genetic Distance of Various Snake Melon Cucumis melo var. flexuosus Cultivars Using Inter Simple Sequence Repeat Technique (ISSR)

Khazraji

Abd

Abdulla

2021

Basrah J. Agric. Sci.

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The experiment have been done on the winter farming season 2020 in one of the farms that belongs to Faris company in Basrah governorate, the technique ISSR have been used to study the genetic distance for twenty one isolates of snake melon Cucumis melo var. flexuosus species. The variations between amplified samples have been revealed after running them on a gel of agarose which have been previously stained by ethidium bromide. Five primers which gave varied product on the agarose have been selected. Those five primers produced 713 bands, both primers UBC 813 and UBC 815 showed the higher numbers of bands reached to 177 while the primer UBC862 showed the least numbers of bands (100) and the bands which showed multi-variations showed (46) bands, and the results primers amplification unique bands their number reached to (14) and five of those bands belong to the primer UBC842 while the primer UBC862 produced three bands while the primer UBC807 did not produced any bands. While it has shown 100% polymorphisms with the primers 813, 815, 842 and 862, and the least polymorphism percentage have shown with the primer UBC807 reached 75%. According to the efficiency primers, the highest efficiency percentage shown with the primer UBC813 and 815 reached to 24.82% and the least percentage shown 14.02% by the primer UBC862. Cluster analysis showed the effect on the variance of the studied cultivars.

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“…The highest polymorphic percentage was obtained from primers ISSR-5 and ISSR-10. This value can be used as a reference for primary selection that can obtain multiple polymorphism sites (Akash et al 2020). Molecular characterization of melon landraces in Iran used 23 ISSR primers.…”

Section: Discussionmentioning

confidence: 99%

Assessment of maturity stage and stability of new Indonesian melon cultivar ‘Melona’ based on ISSR markers and morphological characteristics

et al. 2023

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Abstract. Yusuf AF, Alfiyani A, Salsabila TAS, Kusnanda PS, Hidayati IN, Daryono BS. 2023. Assessment of maturity stage and stability of new Indonesian melon cultivar ‘Melona’ based on ISSR markers and morphological characteristics. Biodiversitas 24: 308-316. Market preferences for horticultural products determine the product’s selling value and absorption. One market criterion often used for the quality of horticultural products is color and maturity level. Color differences can provide a representation of the metabolites contained in a product. This study provides a standard for assessing melons' maturity level, which will be helpful as a benchmark for Indonesian consumer acceptance. In addition, this study also provides information on the stability of the character of the new cultivar 'Melona' from several generations of crosses to obtain superior cultivars. Assessment of maturity level based on color development in 'Melona' is divided into five stages. In the first and second stage, fruit was dominated by green color with Increasing fruit volume. The color change begins in the third stage. in the third stage and the following phases, there is an increase in the accumulation of beta-carotene and characterized by the dominance of orange instead of green. Maximum ripe of ‘Melona’ is characterized by strong yellow lobes, creamy-white interlobes, and orange flesh color. A molecular analysis employing four ISSR primers (UBC-807, UBC-808, UBC-811, and UBC-824) resulted in high uniformity and low genetic variation in the hybrid and parent lines genotype. Analysis resulted a total of 42 DNA fragments with only one polymorphic DNA fragment. The polymorphic DNA fragment was 2,300 bp in size and was found in the female accession 'Melona' and the hybrid 'Melona' based on the UBC-808 primer. The numerical phenetic relationship between male, female and hybrid accession melon cultivars is very close (above 97.6%) and indicated that the melona cultivars were stable.

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Genetic diversity among snake melon landraces (Cucumis Melo Var. Flexuosus) using molecular descriptors

Cited by 8 publications

References 24 publications

Assessment of genetic diversity among Iranian Aegilops triuncialis accessions using ISSR, SCoT, and CBDP markers

Assessment of genetic diversity among Iranian Aegilops triuncialis accessions using ISSR, SCoT, and CBDP markers

The Determination of the Genetic Distance of Various Snake Melon Cucumis melo var. flexuosus Cultivars Using Inter Simple Sequence Repeat Technique (ISSR)

Assessment of maturity stage and stability of new Indonesian melon cultivar ‘Melona’ based on ISSR markers and morphological characteristics

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