Genetic Detection of Extended-Spectrum β-Lactamase-Containing
            <i>Escherichia coli</i>
            Isolates from Birds of Prey from Serra da Estrela Natural Reserve in Portugal

Pinto, Luís; Radhouani, Hajer; Coelho, Catarina; Costa, Paulo Martins da; Simões, Roméo Rocha; Brandão, Ricardo; Torres, Carmén; Igrejas, Gilberto; Poeta, Patrícia

doi:10.1128/aem.02761-09

Cited by 55 publications

(52 citation statements)

References 21 publications

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“…It is notable that 58.5% of birds in the non-aquatic-associated group (Table 2) consisted of birds of prey, which all tested negative for ESBL-or AmpC-producing E. coli. This result is in contrast to results of a recent study from Portugal (21), where 32 out of 119 samples (26.9%) originating from birds of prey tested positive for European herring gull (3), great black-backed gull (2), black-headed gull (1), domesticated duck (1), herring gull (1), lesser black-backed gull (1), mute swan (1) ESBL-producing E. coli. Although the sample size in our study was small (n ϭ 24), it may indicate a relatively low prevalence of ESBLs in birds of prey in The Netherlands.…”

Section: Discussioncontrasting

confidence: 56%

Characteristics of Cefotaxime-Resistant Escherichia coli from Wild Birds in The Netherlands

Veldman

Tulden

Kant

et al. 2013

Appl Environ Microbiol

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bCloacal swabs from carcasses of Dutch wild birds obtained in 2010 and 2011 were selectively cultured on media with cefotaxime to screen for the presence of extended-spectrum ␤-lactamase (ESBL)/AmpC-producing Escherichia coli. Subsequently, all cefotaxime-resistant E. coli isolates were tested by broth microdilution and microarray. The presence of ESBL/AmpC and coexisting plasmid-mediated quinolone resistance (PMQR) genes was confirmed by PCR and sequencing. To determine the size of plasmids and the location of ESBL and PMQR genes, S1 pulsed-field gel electrophoresis (PFGE) was performed on transformants, followed by Southern blot hybridization. The study included 414 cloacal swabs originating from 55 different bird species. Cefotaxime-resistant E. coli isolates were identified in 65 birds (15.7%) from 21 different species. In all, 65 cefotaxime-resistant E. coli ESBL/AmpC genes were detected, mainly comprising variants of bla CTX-M and bla CMY-2 . Furthermore, PMQR genes [aac(6=)-lb-cr, qnrB1, and qnrS1] coincided in seven cefotaxime-resistant E. coli isolates. Overall, replicon typing of the ESBL/AmpC-carrying plasmids demonstrated the predominant presence of IncI1 (n ‫؍‬ 31) and variants of IncF (n ‫؍‬ 18). Our results indicate a wide dissemination of ESBL and AmpC genes in wild birds from The Netherlands, especially among aquatic-associated species (waterfowl, gulls, and waders). The identified genes and plasmids reflect the genes found predominantly in livestock animals as well as in humans.

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Section: Discussioncontrasting

confidence: 56%

Characteristics of Cefotaxime-Resistant Escherichia coli from Wild Birds in The Netherlands

Veldman

Tulden

Kant

et al. 2013

Appl Environ Microbiol

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“…In recent years, different reports have alerted us to the spread of VRE and/or ESBL-containing E. coli isolates from clinical, farm and wild animals in different European countries (Mallon et al, 2002;Torres et al, 2003;Bonnedahl et al, 2010;Literak et al, 2010;Pinto et al, 2010). Although the percentage of vanA isolates from wild birds in the Azores was lower when compared with other studies performed in mainland Portugal in farm and wild animals (Poeta et al, 2005a;Gonçalves et al, 2010;Radhouani et al, 2010b, Radhouani et al, 2010c, our isolates showed a phenotype of multi-resistance.…”

Section: Discussioncontrasting

confidence: 47%

“…Although previous reports have demonstrated the presence of ESBL-producing E. coli in faecal samples of wild animals in mainland Portugal Poeta et al, 2009;Pinto et al, 2010;Radhouani et al, 2010a), a recent study highlighted the absence of these strains in the song thrush . In the past few years there has been an increase in the detection of ESBLs, mainly of the CTX-M group, in wild animals (Poeta et al, 2009;Bonnedahl et al, 2010;Pinto et al, 2010;Radhouani et al, 2010a).…”

Section: Discussionmentioning

confidence: 94%

“…In the past few years there has been an increase in the detection of ESBLs, mainly of the CTX-M group, in wild animals (Poeta et al, 2009;Bonnedahl et al, 2010;Pinto et al, 2010;Radhouani et al, 2010a). In this study, the ESBLcontaining E. coli isolate, classified in the A phylogroup, was more sensitive to the antimicrobial compounds tested than ESBL-positive E. coli isolates recovered from other wild animals in mainland Portugal Poeta et al, 2009;Pinto et al, 2010;Radhouani et al, 2010a). Moreover, the b-lactamase genes detected in our isolate (bla CTX-M-14 and bla SHV-12 ) were also found in wild birds in Sweden (Bonnedahl et al, 2010) and Poland (Literak et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

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Molecular characterization of vancomycin-resistant enterococci and extended-spectrum β-lactamase-containingEscherichia coliisolates in wild birds from the Azores Archipelago

et al. 2011

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To study the prevalence of vancomycin-resistant enterococci (VRE) and extended-spectrum b-lactamase (ESBL)-containing Escherichia coli isolates, and the mechanisms of resistance implicated, 220 faecal samples from wild birds were collected between 2006 and 2010 in the Azores Archipelago. Samples were spread on SlanetzÁBartley agar plates supplemented with 4 mg/l vancomycin and on Levine agar plates supplemented with 2 mg/l cefotaxime for VRE and ESBL-containing E. coli isolation, respectively. vanA-containing enterococcal isolates (four Enterococcus faecium and two Enterococcus durans) and vanC-1 Enterococcus gallinarum isolates were detected in six and seven faecal samples, respectively. VRE isolates showed ampicillin (n 011), ciprofloxacin (n 09), tetracycline (n06), erythromycin (n 05), quinupristin/dalfopristin (n 03) and high-level kanamycin resistance (n 01). The tet(L) and/or tet(M) gene was found in all tetracycline-resistant isolates and the erm(B) gene in all erythromycin-resistant isolates. Three vanAcontaining E. faecium and two E. gallinarum presented specific sequences of the Tn5397 transposon. Four VRE isolates harboured the ace virulence gene. One faecal sample revealed one ESBL-containing E. coli isolate that belongs to the A phylogenetic group, showed a phenotype of resistance to b-lactams and tetracycline, and harboured the bla CTX-M-14 , bla SHV-12 and the tet(A) genes. To our knowledge, this is the first study to focus on defining the prevalence of VRE and/or ESBL-containing E. coli strains in wild birds from the Azores. The data recovered are essential to improve knowledge about the dissemination of resistant strains through wild ecosystems and their possible implications by transferring these resistances to other animals or to humans.

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“…As for companion animals, ESBL-producing bacteria have been found in urinary tracts of dogs and cats [10], wound sites, and preputial discharge of companion animals [11], cases of cholangio-hepatitis in dogs [12], and in dog feces [13]. In wild animals, ESBLs have been detected in fecal samples from birds of prey [14], urban brown rats [15], and in seagulls [16].…”

Section: Extended-spectrum Beta-lactamases: Introductionmentioning

confidence: 99%

An overview of extended-spectrum beta-lactamases in veterinary medicine and their public health consequences

Nobrega

Brocchi

2014

J Infect Dev Ctries

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Serious human and animal infections caused by bacteria are usually treated with beta-lactams. Extended-spectrum beta-lactamases (ESBLs) constitute the most clinically and economically important enzymes that are able to hydrolyze and inactivate beta-lactam antibiotics in veterinary medicine. The spread of ESBLs represents a serious threat to healthcare systems, drastically undermining therapeutic options. The relationship between drug usage and the emergence of resistance has been extensively reported. Nevertheless, the use of antimicrobials in veterinary medicine and the emergence of ESBLs in animals remains a matter of debate. Moreover, there is still controversy about whether antibiotic usage in farm animals poses a potential public health risk. This review will (i) deal with aspects related to the presence of ESBLs in veterinary medicine, (ii) its link with human medicine, and (iii) discuss strategies to be implemented to preserve antimicrobial effectiveness. New insights relative to old questions concerning antimicrobial use in domestic animals are also presented.

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Genetic Detection of Extended-Spectrum β-Lactamase-Containing Escherichia coli Isolates from Birds of Prey from Serra da Estrela Natural Reserve in Portugal

Cited by 55 publications

References 21 publications

Characteristics of Cefotaxime-Resistant Escherichia coli from Wild Birds in The Netherlands

Characteristics of Cefotaxime-Resistant Escherichia coli from Wild Birds in The Netherlands

Molecular characterization of vancomycin-resistant enterococci and extended-spectrum β-lactamase-containingEscherichia coliisolates in wild birds from the Azores Archipelago

An overview of extended-spectrum beta-lactamases in veterinary medicine and their public health consequences

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