Genetic Correlation of Virulent Salmonella Serovars (Extended Spectrum β-Lactamases) Isolated from Broiler Chickens and Human: A Public Health Concern

Abstract

This study aimed to detect the virulent Salmonella serovars (including ESBLs producing) isolated from broiler chickens and humans. Three hundred broilers and sixty human fecal samples were bacteriologically examined. Thirty (10%) and fourteen (23.4%) Salmonella isolates were recovered from broiler and human samples, respectively. The most predominant serovar was S. enteritidis and S. typhimurium. All Salmonella isolates were confirmed by conventional PCR-based invA and ompA genes. Multidrug resistant (MDR) isolates were screened for the detection of adrA and csgD biofilm-associated genes, which were found in all isolated serovars except one S. typhimurium and 2 S. infantis of chicken isolates that were devoid of the adrA gene. Moreover, MDR isolates were screened for detection of seven resistance genes including ESBLs and other classes of resistance genes. Chicken isolates harbored blaTEM, int1, blaCTX and qnrS genes as 100, 27.8, 11.1 and 11.1%, respectively, while all human isolates harbored blaTEM, int1 and int3 genes. The genetic correlations between virulent Salmonella serovars (including antimicrobial resistance) avian and human origins were compared. In conclusion, the high prevalence of virulent ESBL producing Salmonella serovars in broilers and humans with genetic correlations between them might be zoonotic and public health hazards.

Controlling Multi-Drug-Resistant Traits of Salmonella Obtained from Retail Poultry Shops Using Metal–Organic Framework (MOF) as a Novel Technique

“…The findings of the current study are similar to those reported by Ahmed et al [43], who obtained Salmonella by rate of 18.7 and 16.9%., respectively. Meanwhile, our findings were unlike those found by Orabi et al [44], who detected Salmonellae in a rate of 10.0%. Our observations propose the prospect that the environment has a potential role in circulating the pathogen between animals and human populations.…”

“…Furthermore, isolates obtained from cutting knives were considerably resistant to gentamycin (100.0%), followed by those obtained from intestinal swabs, hand swabs, and cutting boards at 93.3, 90.1%, and 8.9%, respectively. Similar findings were investigated by Doyle et al [55], Zishiri et al [56], Nabil and Younis [57] and Orabi et al [44]. Notably, the exuding rates of microbial resistance to most of the investigated antibiotics in this study were not surprising, which suggests the abuse and/or overuse of most common therapeutic antimicrobial classes utilized in the field of the poultry industry with risk of transmission of this resistance to human traits through the food chain [58,59].…”

Detection of Virulence and β-lactamase resistance genes of non-typhoidal Salmonella isolates from human and animal origin in Egypt "one health concern"

“…Many authors in previous studies agree with us in the opinion that the most common serotypes of salmonella in humans and animals were S. typhimurium and S. enteritidis [ 15 , 18 – 20 ]. However, a previous study in Ethiopia demonstrated that the most common serovar were S. dublin and S. Virchow [ 21 ].…”

Occurrence, Antimicrobial Resistance, and Virulence Profiles of Salmonella Serovars Isolated from Wild Reptiles in South Africa

“…Antibiotics used in this study were streptomycin (30 μg), ciprofoxacin (5 μg), nalidixic acid (30 μg), gentamicin (10 μg), and kanamycin (30 μg). Resistance to two or more antimicrobials of diferent classes was considered to be multidrug-resistant (MDR) [26].…”