Genetic characterization of selected Trifolium species as revealed by nuclear DNA content and ITS rDNA region analysis

Vižintin, Liliana; Javornik, Branka; Bohanec, B.

doi:10.1016/j.plantsci.2005.12.007

Cited by 44 publications

(54 citation statements)

References 28 publications

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“…However, all the members of the section Trifolium remained under the main cluster in our study. In another study (Vizintin et al, 2006), the groups of the section Trifolium obtained also supported our results. The last section Vesicaria members T. resupinatum, T. physodes, and T. fragiferum were found to be genetically close to each other and they were placed as a separate subgroup between the Lotoidea and Chronosemium section subclusters under the main cluster ( Figure 5).…”

Section: Discussionsupporting

confidence: 87%

Assessment of genetic diversity in naturally growing 29 Trifolium L. taxa from Bolu Province using RAPD and SSR markers

Uslu¹,

Ertuğrul²,

Babaç³

2013

Turk J Biol

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Two molecular marker technologies, RAPD and SSR, were used to determine the genetic diversity of 29 taxa of Trifolium L. collected from Bolu Province. The analysis was carried out using 11 RAPD and 5 SSR markers. A total of 446 and 331 fragments were produced by RAPD and SSR markers, respectively. The amplification products were 300-3500 bp with RAPD and 125-3000 bp with SSR markers. These bands were scored as absence and presence for all taxa. Data obtained were used to estimate genetic similarity using Jaccard's coefficient, and dendrograms were constructed by the UPGMA method using either RAPD and SSR markers individually or as combined sets of data. The highest genetic similarity values obtained were 0.33 between T. hirtum All. and T. constantinapolitanum Ser. for RAPD and 0.49 between T. medium L. var. medium and var. eriocalycinum Hausskn. for SSR. The dendrograms produced from combined sets of RAPD and SSR data revealed 2 main clusters. One contained members of the sections Chronosemium Ser., Lotoidea L., and Vesicaria Ser., and the other had only the section Trifolium. The results suggest that the use of 2 different molecular markers gives the most reliable genetic data for Trifolium taxa.

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Section: Discussionsupporting

confidence: 87%

Assessment of genetic diversity in naturally growing 29 Trifolium L. taxa from Bolu Province using RAPD and SSR markers

Uslu¹,

Ertuğrul²,

Babaç³

2013

Turk J Biol

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“…Zohary and Heller (1984) considered x = 8 to be the basic chromosome number of the genus for 80% of the species (Vižintin et al 2006) while x = 7, 6 and 5 are derived states.…”

Section: Genome and Karyotypementioning

confidence: 99%

“…Rizza et al (2007) Sato et al (2005) deduced a haploid genome size of 440 Mb, as estimated by measuring the nuclear DNA content using flow cytometry (two red clover plants with 0.89 and 0.91 pg/2C), with 1 pg of DNA equivalent to 980 Mb (Bennett et al 2000). Ellison et al (2006) and Vižintin et al (2006) reported results of detailed and comprehensive studies of the molecular phylogenetic framework for the genus Trifolium. The latter authors assessed the interspecific relationships among Eurasian clovers based on sequence polymorphisms of the internal transcribed spacer region of nuclear ribosomal DNA (rDNA).…”

Section: Genome and Karyotypementioning

confidence: 99%

Modern methods for genetic improvement of Trifolium pratense.

Řepková¹,

Nedĕlník²

2014

CZECH J GENET PLANT

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This review focuses on trends in genetic improvement of a significant representative forage crop, Trifolium pratense (red clover) classified taxonomically into the agronomically outstanding family Fabaceae. Red clover breeding is aimed at improving traits like persistency, resistance to biotic and abiotic factors, forage yield and quality characteristics such as protein quality and stability. Isoflavone content in forage is important for cattle reproduction. Interspecific hybridization of red clover with the related wild species T. medium was used for the introgression of useful traits into red clover. The breeding strategy for the new variety Pramedi included hybrid plants with different DNA contents, repeated backcrosses with red clover, followed by family selection. New techniques of molecular genetics are becoming available to breeders for transferring key and complex traits into improved red clover varieties. Techniques such as marker-assisted selection and candidate gene identification can increase the speed and precision with which traits may be selected. Comparative sequence data analyses helped to identify genes for polyphenol oxidase enzymes and isoflavone synthase in red clover useful for DNA genotyping of individuals and breeding of improved high-quality red clover varieties.Keywords: candidate gene; genetic marker; molecular genetics; red clover; wide hybridization Throughout the history of plant breeding, breeders have endeavoured to improve crop yields and quality, always in the context of the latest knowledge and demands of users. This paper focuses on trends in genetic improvement of a significant representative forage crop, Trifolium pratense L. (red clover), by modern methods. The review summarizes current knowledge of the red clover genome and prospects for using molecular genetics to improve that genome.The genus Trifolium L. is classified taxonomically into the tribe Trifoliae of the large and agronomically outstanding family Fabaceae. It comprises approximately 240 species (Zohary & Heller 1984) of annual and perennial herbs commonly known as clovers, both wild and cultivated. Red clover is an important forage legume that is widely cultivated in most temperate regions and is grown in pastures for grazing, sown as a companion crop, and harvested for hay (Smith et al. 1985; Taylor & QueenSberry 1996). It is also used as a green manure crop because of its high nutrient content resulting partly from symbiosis with nitrogen-fixing bacteria of the genus Rhizobium.In Europe and worldwide, red clover breeding is focused on improving traits like persistency, resistance to biotic and abiotic factors, and forage yield and quality characteristics such as protein quality and stability, fermentable carbohydrate content, and cell wall digestibility. The benefits of protecting plant proteins from degradation in the rumen have been established. Polyphenol oxidase (PPO) enzymes catalyse the aerobic conversion of monophenols to o-phenols and finally to o-quinones (Macheix et al. 1991) and are present in sign...

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“…The genome size (0.85 pg) was previously estimated for this species using Trifolium repens L. cv. Milo as standard (VI@INTIN et al 2006). …”

Section: Determination Of Nuclear Dna Content By Flow Cytometrymentioning

confidence: 99%

“…Although molecular data are considered the most convincing data set, which can be used to identify phylogenetic relationships, a combination of different data sets, including genome size data, provides useful information on genetic similarity and taxonomic position (VI@INTIN et al 2006). Most reports (LEITCH and BENNETT 2002) confirmed that genome size is usually a value with limited variations within a single species and could be considered characteristic of the species.…”

Section: Introductionmentioning

confidence: 99%

Genetic characterization of Genista sericea Wulfen (Cytiseae – Fabaceae) as revealed by nuclear DNA content and its nrDNA region analysis

Vižintin¹,

Kosovel²,

Chiapella³

2012

Acta Botanica Croatica

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Summary -Genista sericea Wulfen, a northern Illyrian amphi-Adriatic species, presents a certain morphological variability. To clarify whether the genetic variations support the morphological differences among accessions of different geographic origin, analysis of nuclear DNA content and polymorphism of the internal transcribed spacer (ITS) dataset was studied. The variation in nuclear DNA content of G. sericea var. sericea and var. rigida is minimal (2.09 and 2.08 pg/nucleus respectively) and is correlated with equal chromosome numbers in both varieties. Intraspecific variability of the ITS region was studied on 13 accessions of G. sericea, 6 belonging to var. sericea and 7 to var. rigida. These accessions were analyzed in comparison to closely related species already studied. ITS sequences of G. sericea revealed large polymorphism and formed two main clusters. One cluster (6 accessions) comprehends var. sericea of northern Italy, Slovenia and northern Croatia; the other cluster (7 accessions) includes five accessions of var. rigida from southern Croatia and Montenegro and two from the Pollino massif (southern Italy). The later two accessions considerably differed from other accessions of var. rigida. This genetic analysis supports the previous assumptions, which subdivided G. sericea into at least two taxa. On the basis of the results presented, it is here suggested that the subdivision of G. sericea into var. sericea and var. rigida should be maintained.

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Genetic characterization of selected Trifolium species as revealed by nuclear DNA content and ITS rDNA region analysis

Cited by 44 publications

References 28 publications

Assessment of genetic diversity in naturally growing 29 Trifolium L. taxa from Bolu Province using RAPD and SSR markers

Assessment of genetic diversity in naturally growing 29 Trifolium L. taxa from Bolu Province using RAPD and SSR markers

Modern methods for genetic improvement of Trifolium pratense.

Genetic characterization of Genista sericea Wulfen (Cytiseae – Fabaceae) as revealed by nuclear DNA content and its nrDNA region analysis

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