Genetic characterization of<i>Salix alba</i>L. and<i>Salix fragilis</i>L. by means of different PCR-derived marker systems

Meneghetti, Stefano; Barcaccia, Gianni; Paiero, P.; Lucchin, Margherita

doi:10.1080/11263500701627448

Cited by 19 publications

(29 citation statements)

References 18 publications

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“…Spontaneous hybridization is determined by dioecism and is affected by variation of flowering times in different species. In the genus Salix, both balanced and introgressed hybrids seem to dominate when considering only morphological traits, but studies based on biochemical and molecular markers revealed that genetic differentiation within populations is low as well as genetic flow between populations [6,7]. This finding is consistent with historical data available for forest trees with a dioecious-type breeding system and wind-dispersed seeds [8].…”

Section: Introductionsupporting

confidence: 79%

“…New biotechnological developments have expanded the range of plant DNA polymorphism assays for characterizing and investigating germplasm resources and genetic relatedness, as well as for linkage mapping, gene targeting and assisted breeding. The area of willow research showing the greatest development with respect to the use of molecular marker technology is that of population genetics [7,13,14,18,[21][22][23][24], whereas information on structural genomics is scanty. In fact, nothing is known about willow genomic constitution and origin, and whether species are autopolyploid or allopolyploid remains an open question.…”

Section: Introductionmentioning

confidence: 99%

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Genetic Segregation and Genomic Hybridization Patterns Support an Allotetraploid Structure and Disomic Inheritance for Salix Species

et al. 2014

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Abstract:The Salix alba L. (white willow)-Salix fragilis L. (crack willow) complex includes closely related polyploid species, mainly tetraploid (2n = 4x = 76), which are dioecious and hence obligate allogamous. Because little is known about the genome constitution and chromosome behavior of these pure willow trees, genetic analysis of their naturally occurring interspecific polyploid hybrids is still very difficult. A two-way pseudo-testcross strategy was exploited using single-dose AFLP markers in order to assess the main inheritance patterns of tetraploid biotypes (disomy vs. tetrasomy) in segregating populations stemmed from S. alba × S. fragilis crosses and reciprocals. In addition, a genomic in situ hybridization (GISH) technology was implemented in willow to shed some light on the genome structure of S. alba and S. fragilis species, and their hybrids (allopolyploidy vs. autopolyploidy). The frequency of S. alba-specific molecular markers was almost double compared to that of S. fragilis-specific ones, suggesting the phylogenetic hypothesis of S. fragilis as derivative species from S. alba-like progenitors. Cytogenetic observations at pro-metaphase revealed about half of the chromosome complements being OPEN ACCESS Diversity 2014, 6 634 less contracted than the remaining ones, supporting an allopolyploid origin of both S. alba and S. fragilis. Both genetic segregation and genomic hybridization data are consistent with an allotetraploid nature of the Salix species. In particular, the vast majority of the AFLP markers were inherited according to disomic patterns in S. alba × S. fragilis populations and reciprocals. Moreover, in all S. alba against S. fragilis hybridizations and reciprocals, GISH signals were observed only on the contracted chromosomes whereas the non-contracted chromosomes were never hybridized. In conclusion, half of the chromosomes of the pure species S. alba and S. fragilis are closely related and they could share a common diploid ancestor, while the rest of chromosomes are morphologically differentiated in either S. alba or S. fragilis and they should derive from distinct diploid ancestors.

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Section: Introductionsupporting

confidence: 79%

Section: Introductionmentioning

confidence: 99%

Genetic Segregation and Genomic Hybridization Patterns Support an Allotetraploid Structure and Disomic Inheritance for Salix Species

et al. 2014

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“…The Eco, Pst and Mse primers were called in tables and figures as E, P and M, respectively. The cycling conditions were 1 cycle of 45 s at 94°C, 30 s at 65°C, 1 min at 72°C and a touch-down profile (13 cycles with -0.7°C/cycle) for the annealing temperature and finally by an extension cycle of 5 min at 72°C [45,46].…”

Section: Aflp Sampl and M-aflp Analysismentioning

confidence: 99%

“…Each 20 ul PCR contained 0.5 ul of the preamplified DNA, 50 ng of labelled EcoRl or Pstl primer, 30 ng of unlabelled Msel primer, 2 ul of PCR buffer, 4 mM dNTPs and 0.4 U of Taq DNA polymerase [46].…”

Section: Aflp Sampl and M-aflp Analysismentioning

confidence: 99%

Clones Identification and Genetic Characterization of Garnacha Grapevine by Means of Different PCR-Derived Marker Systems

Meneghetti¹,

Costacurta²,

Frare³

et al. 2010

Mol Biotechnol

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“…Various kinds of DNA markers have been used to analyse Salix species, including dominant markers such as AFLPs, ISSR and RAPDs (Meneghetti et al 2007), as well as co-dominant markers such as SSRs (Perdereau et al 2014). Salix species have also been extensively studied with maternallyinherited chloroplast markers (Palmé et al 2003, Perdereau et al 2014, which enables the differentiation of lineages, as well as the direction of hybridisation.…”

Section: Introductionmentioning

confidence: 99%

Genetic analysis of Latvian Salix alba L. and hybrid populations using nuclear and chloroplast DNA markers

Ruņģis¹,

Laiviņš²,

Gailīte³

et al. 2017

iForest

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Latvia is at the northern border of the species range of S. alba L. in Europe, and there has been some debate whether the Latvian populations of S. alba are autochthonous, as this species has long been planted in Latvia as an ornamental tree in gardens, parks and roadsides. In addition, there is increasing interest in the use of several Salix species (including S. alba) as bioenergy crops. Natural S. alba stands throughout Latvia, as well as stands of possibly hybrid origin were analysed using nuclear and chloroplast markers. Our results showed that S. alba populations are probably natural, and that the rate of vegetative reproduction is low, supporting the evidence that Latvia is within the natural range of S. alba. These results provide the basis for the identification of possibly introduced or artificially regenerated stands of S.alba in Latvia. In addition, our results confirm that S. alba hybridises with S. fragilis, and that natural stands including hybrid individuals can be established. The analysis of chloroplast markers indicated that the predominant hybridisation occurs by fertilisation of S. fragilis by S. alba pollen; however, the extent of haplotype sharing between these two species should be further investigated.

show abstract

Genetic characterization ofSalix albaL. andSalix fragilisL. by means of different PCR-derived marker systems

Cited by 19 publications

References 18 publications

Genetic Segregation and Genomic Hybridization Patterns Support an Allotetraploid Structure and Disomic Inheritance for Salix Species

Genetic Segregation and Genomic Hybridization Patterns Support an Allotetraploid Structure and Disomic Inheritance for Salix Species

Clones Identification and Genetic Characterization of Garnacha Grapevine by Means of Different PCR-Derived Marker Systems

Genetic analysis of Latvian Salix alba L. and hybrid populations using nuclear and chloroplast DNA markers

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