Clones Identification and Genetic Characterization of Garnacha Grapevine by Means of Different PCR-Derived Marker Systems

Abstract

This study uses PCR-derived marker systems to investigate the extent and distribution of genetic variability of 53 Garnacha accessions coming from Italy, France and

Ampelographic and Molecular Characterisation of Aglianico Accessions (Vitis vinifera L.) Collected in Southern Italy

“…For example, the fingerprint of accession 7 revealed genetic diversity, thus confirming the greater phenotypic variability observed by ampelographic analysis. Indeed, it has to be noted that, as already reported in the literature, AFLP fingerprinting data are in agreement with the results obtained by ampelographic analyses when a high number of morphological characters are considered (Meneghetti et al, 2010).…”

Inter- and Intra-Varietal Genetic Variability in Vitis vinifera L.

“…The Italian accessions were 6 Tocai rosso from the Vicenza area, 8 Alicante from Sicily and Elba island, 4 Gamay perugino from Perugia province and 10 Cannonau from Sardinia. In order to verify the varietal identity, the analyses based on 11 SSR loci confirmed that only one SSR profile was obtained for the 53 accessions ( Figure 6) (Meneghetti, 2011).…”

“…The amplification products were resolved on ReproGel High-Resolution pre-made acrylamide-bisacrylamide solutions (8% w/v) (GE Healthcare) in modified TBE buffer and detected on a semiautomated DNA sequencer, the ALFexpress-II DNA Analysis System (Amersham Pharmacia Biotech). Markers were visualized automatically by the ALF-win Fragment Analyses 1.09 software and checked by Quantity One 4.6.7 and PD Quest Basic 8.0.1 software (Biorad) (Meneghetti, 2011).…”

“…The majority of AFLP markers were monomorphic but there were some clearly differences: the line 36 and 46 are very similar (two G. blanca genotypes) and line 37 and 49 showed only a different marker (two G. peluda genotypes). Genotypes from left to right: 35= Garnacha tinta, 36= G. blanca, 37= G. peluda, 38= G. roja; from 39 to 45= Garnacha tinta; 46= G. blanca, 47= G. erguida, 48= G. roya, 49= G. peluda; from 50 to 53= G. tinta (Meneghetti, 2011). …”

“…Ampelography, ampelometry, and biochemical traits analysis have been traditionally employed to identify the different biotypes in viticulture (Galet, 1979;Calò & Costacurta, 2004). However, these analyses are based on phenotypic characteristics which can be affected by environmental conditions (Meneghetti, 2011).…”