Genetic characterization of grapevine-infecting Botrytis cinerea isolates from Argentina

Effect of temperature on the morphological characteristics of Botrytis cinerea and its correlated with the genetic variability

“…The diameter of each mycelial growth was measured at 7 d at the temperatures of 4,12 and 28 °C , in three perpendicular directions without opening the Petri dishes, and the mycelial area was calculated. The results were expressed in square centimeters.…”

“…Fruits were wounded (3 mm×3 mm×3 mm) with a punch on two opposite sides of each fruit, and inoculated with 20 µL of each conidial suspension (10 6 conidia per mL) and 20 µL of water as control [19] . After 7 d of incubated at temperatures of 4,12 and 28 °C , by measuring the diameter of the decayed area as the mean of its width and length and computing the total area of disease. Areas of disease were analyzed by analysis of variance.…”

“…A cut 4 mm mycelial disc from a 3-day-old PDA culture was placed in the center of each plate. The dishes were incubated for 7 d at 4,12 and 28 °C . Colony diameters were measured in three perpendicular directions without opening the Petri dishes and the inhibition of mycelial growth was calculated on the basis of the area in compared with controls: % inhibitions=[(φ control−φ treatment)/φ control]伊100.…”

“…cinerea is a haploid and heteroallelic ascomycete [12] . Analysis genetic such as RFLP-PCR [13,14] , ARMS-PCR [15] , allelespecific PCR [16] , RAPD-PCR [4] , nested PCR, and detection transposable elements (TEs) [13] , amongst other molecular typing techniques, have characterized to B. cinerea.…”

“…Recent studies of DNA polymorphism of genes of isolates of B. cinerea have grouped them in two phylogenetic species groups. Group I strains belonged exclusively to the vacuma type, whereas Group II strains included both vacuma and transposa types [4,12] . The phylogenetic analyses between Group I and Group II have shown differences in morphology, phenology and host range but has not identified any differentiation between vacuma and transposa strains within Group II [17] .…”