Molecular identification and characterization of Botrytis cinerea associated to the endemic flora of semi-desert climate in Chile

Notte, Ana-Maria; Plaza, Verónica; Marambio-Alvarado, Bárbara; Olivares-Urbina, Lila; Poblete‐Morales, Matías; Silva-Moreno, Evelyn; Castillo, Luís

doi:10.1016/j.crmicr.2021.100049

Cited by 11 publications

(17 citation statements)

References 76 publications

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“…Grey mould can develop fast and the disease can be devastating on the field, in greenhouses, and in post-harvest (Rui andHahn, 2007 andPetrasch et al, 2019). Losses can be severe throughout the production system, at harvest, during transportation, selling, and after final sale (Avenot et al, 2020 andNotte et al, 2021).…”

Section: Causal Agent Of Strawberry Grey Mouldmentioning

confidence: 99%

“…Primary identification of B. cinerea is from its atypical spore and macroconidiophore structure (Alfonso et al, 2000 andWilliamson et al, 2007). This is a specialized structure comprising a terminal cluster of synchronously produced hydrophobic conidia, borne on a welldeveloped macroconidiogenous hypha, which resembles a bunch of grapes (Williamson et al, 2007;Avenot et al, 2020 andNotte et al, 2021). These hyphae originate from a cushionshaped stroma, which forms just beneath the host surface (Boff et al, 2001;Williamson et al, 2007;Feliziani and Romanazzi, 2016).…”

Section: Causal Agent Of Strawberry Grey Mouldmentioning

confidence: 99%

“…Macroconidia are oval, dry and hydrophobic, being produced singly on lateral branches for easy dispersal. When mature, conidia are easily detached from the ampullae by rain splash and/or air turbulence, which also serve to distribute them (Williamson et al, 2007;Avenot et al, 2020 andNotte et al, 2021).…”

Section: Causal Agent Of Strawberry Grey Mouldmentioning

confidence: 99%

“…Nevertheless, strawberries are affected by many pathogens such as nematodes, fungi, viruses and bacteria (Petrasch et al, 2019). Moreover, the pathogens with the greatest economic impact are fungi that cause serious damage or death (Fedele et al, 2020 andNotte et al, 2021). Among the fungal pathogens, we mention Botrytis cinerea which represents the major pathogen of strawberries worldwide (Breen et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

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Strawberry Grey Mould, a Devastating Disease Caused by the Airborne Fungal Pathogen Botrytis cinerea

Rhouma

Hajji-Hedfi

Othmen

et al. 2022

Egyptian Journal of Phytopathology

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Strawberry (Fragaria ananassa Duch.) is an important and valuable fruit because of its unique flavor and economic, nutritional and health benefits. It is one of the most consumed berries worldwide. Grey mould of strawberry fruits, caused by the necrotrophic ascomycete Botrytis cinerea Pers.: Fr., is one of the most important diseases. This airborne pathogen has the ability to kill strawberry cells through the production of reactive oxygen species and toxins causing massive production losses at all development stages and even post-harvest. The intensive production of strawberries has created a favorable environment for this disease. Strawberry grey mould management is typically very input-intensive, in particular with respect to chemical fungicides. As a result, the integrated pest management is required to control strawberry grey mould. A thorough understanding of B. cinerea epidemiology and infection processes is needed to guide future efforts in the development of innovative integrated management practices. This review summarizes the current knowledge of taxonomy and morphology, signs and symptoms, disease development, infection process, and control of B. cinerea using integrated disease management.

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Section: Causal Agent Of Strawberry Grey Mouldmentioning

confidence: 99%

Section: Causal Agent Of Strawberry Grey Mouldmentioning

confidence: 99%

Section: Causal Agent Of Strawberry Grey Mouldmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Strawberry Grey Mould, a Devastating Disease Caused by the Airborne Fungal Pathogen Botrytis cinerea

Rhouma

Hajji-Hedfi

Othmen

et al. 2022

Egyptian Journal of Phytopathology

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“…The biological activity of the Paib prototype was evaluated in vitro against C. gloeosporioides, B. cinerea, A. alternata and F. oxysporum. These fungi were selected due to their negative effects in agriculture, related to diseases in crops for human consumption that cause economic and health damage [60][61][62]. The prototype showed an evident effect against the growth of all the pathogenic fungi tested (Figure 5).…”

Section: Pathogenic Fungi In Vitro Growth Inhibitionmentioning

confidence: 99%

Peptaibol Production and Characterization from Trichoderma asperellum and their Action as Biofungicide

Alfaro-Vargas¹,

Bastos-Salas

Muñoz-Arrieta³

et al. 2022

Preprint

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Peptaibols (Paib), are a class of biologically active peptides isolated from soil, fungi and molds, which have interesting properties as antimicrobial agents. Paib production was optimized in flasks by adding sucrose as a carbon source, Aib as an additive amino acid, and F. oxysporum cell debris as an elicitor. Paib were purified, sequenced and identified by HPLC coupled to mass spectrometry. Af-terward, a Paib prototype was prepared with the extracts obtained from the optimized fermenta-tions. The biological activity of these prototypes was evaluated using in vitro and in vivo methods. The prototype inhibited the growth of specific plant pathogens, and it showed inhibition rates similar to those from commercially available fungicides. Growth inhibition rates were 92.2, 74.2, 58.4 and 36.2 % against Colletotrichum gloeosporioides, Botrytis cinerea, Alternaria alternata and Fusarium oxysporum, respectively. Furthermore, the antifungal activity was tested in tomatoes inoculated with A. alternata, the incidence of the disease in tomatoes treated with the prototype was 0%, while the untreated fruit showed a 92.5% of infection. Scanning electron microscopy images showed structural differences between fungi treated with or without Paib. The most visual altera-tions were sunk and shriveled morphology in spores, while the hyphae appeared to be fractured, rough, and dehydrated.

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Preparation and Characterization of a Biopesticide Based on Artemisia herba-alba Essential Oil Encapsulated with Succinic Acid-Modified Beta-Cyclodextrin

Ez-Zoubi,

Ez Zoubi,

Bentata

et al. 2023

Journal of Chemistry

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Numerous essential oils have been researched as biopesticides because of their effectiveness and environmental safety. Nevertheless, encapsulation is necessary to improve its physical, chemical, and biological properties. Therefore, this paper aims to investigate the physicochemical characteristics and antifungal activity of the Artemisia herba-alba essential oil (HAEO) encapsulated in succinic acid-modified β-cyclodextrin (SACD). Hydrodistillation was used to extract a yellowish oil from the plant A. herba-alba, and gas chromatography coupled to mass spectrometry was performed to determine the chemical composition. The scanning electron microscope, Fourier transform infrared, thermogravimetric analysis, and docking studies were combined to validate the molecular inclusion of HAEO with SACD. The antifungal activity was examined against Botrytis cinerea by direct contact with a potato dextrose agar. The results showed that the main compound in HAEO was α-thujone (65.0%). Furthermore, HAEO has been successfully incorporated into SACD with a binding energy value of −5.3 kJ·mol−1, and its thermal stability improved. At a 0.1% concentration, HAEO in encapsulated form showed a higher ability to inhibit B. cinerea (38.34%), compared to EO in free form (12.00%). Thus, these findings produced evidence that the strategy of encapsulating EOs by SACD can develop novel B. cinerea inhibitors and a promising alternative biopesticide.

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Molecular identification and characterization of Botrytis cinerea associated to the endemic flora of semi-desert climate in Chile

Cited by 11 publications

References 76 publications

Strawberry Grey Mould, a Devastating Disease Caused by the Airborne Fungal Pathogen Botrytis cinerea

Strawberry Grey Mould, a Devastating Disease Caused by the Airborne Fungal Pathogen Botrytis cinerea

Peptaibol Production and Characterization from Trichoderma asperellum and their Action as Biofungicide

Preparation and Characterization of a Biopesticide Based on Artemisia herba-alba Essential Oil Encapsulated with Succinic Acid-Modified Beta-Cyclodextrin

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