Genetic characterisation of adenovirus type 8 isolated in Hiroshima city over a 15 year period

Abstract

Aims: To investigate the genetic differences among the strains of adenovirus type 8 (Ad8) circulating in Hiroshima city, Japan, and to study their circulation pattern. Methods: One hundred and twenty nine strains of adenovirus type 8 (Ad8) were isolated in Hiroshima City over a 15 year period (1983-97) from patients with keratoconjunctivitis, and analysed with six restriction enzymes-BamHI, HindIII, PstI, SacI, SalI, and SmaI-to investigate possible relations among the isolates and their genetic variability. Seven hypervariable regions of the hexon gene that carry the type specific epitope were also sequenced to investigate the variation among the genome types. Results: Restriction endonuclease analyses yielded three known genome types (Ad8A, 13 samples; Ad8B, seven samples; and Ad8E, 35 samples) and a novel genome type (Ad8I, 74 samples). Ad8A, Ad8B, and Ad8E were closely related, with 96% homology, whereas Ad8I had only 71% homology. Ad8A, Ad8B, and Ad8E shared 91.8% and 96.4% homology with regard to their amino acid and nucleotide sequences, respectively, with the isolate 1127 (accession no X74663). However, when compared with Ad8A, Ad8B, Ad8E, and isolate 1127, Ad8I shared only 62.7% and 69.9% homology with regard to amino acid and nucleotide sequences, respectively. Ad8A, Ad8B,and Ad8E had a unique 31 amino acid deletion in the hypervariable region 1 of the hexon gene, whereas Ad8I had a 33 residue deletion. The Ad8E strain that circulated from 1984 to 1995 was stable among the study population. Ad8I was isolated from an outbreak of epidemic keratoconjunctivitis in 1995 and was also isolated from sporadic cases until 1997. Conclusions: These results confirmed that genetic variability occurs in Ad8 in the microenvironment and revealed the emergence of a new genome type (Ad8I).

Molecular Epidemiology of Adenovirus Infection among Pediatric Population with Diarrhea in Asia

“…Until now, 51 serotypes have been identified. Among them adenovirus type 3, 4, 7, 8, 19 and 37 have been found to be responsible for keratoconjunctivitis worldwide (1,8). Interestingly, in the present study Ad3 was detected with the rather high incidences 4.0% and 30% and Ad8 with 8.0% and 20.0% in Japan and Korea, respectively.…”

Molecular epidemiology of adenovirus strains isolated from patients with ocular disease in the area of Thessaloniki, Greece (1998-2002)

“…Many investigators have used genomic characterization by different restriction endonucleases to establish genomic variants of Ad8, and around 20 genotypes have been identified [Fujii et al, 1983[Fujii et al, , 1984Kemp et al, 1983;Wadell, 1984]. Genome typing can be also done with subgenus-or type-specific PCR primers Tanaka et al, 2000;Adhikary et al, 2003]. Recently, some methods have suggested that the combination of PCR and RFLP analysis facilitate the typing procedure [Kidd et al, 1996;Saitoh-Inagava et al, 1996].…”

“…Ad8 has a much higher tropism for conjunctival cells than Ad19 and Ad37 and produces severe clinical manifestations and pathological alterations. This type persists in the population and causes sporadic epidemics in many countries [Kemp and Heirholtzer, 1986;Chastel et al, 1988;de Jong et al, 1992;Corsaro et al, 1999;Tanaka et al, 2000;Adhikary et al, 2003]. Many investigators have used genomic characterization by different restriction endonucleases to establish genomic variants of Ad8, and around 20 genotypes have been identified [Fujii et al, 1983[Fujii et al, , 1984Kemp et al, 1983;Wadell, 1984].…”

Detection and Serotyping of Human Adenoviruses from Patients with Influenza-Like Illness in Mongolia

“…Restriction fragment length polymorphism (RFLP) analysis has been commonly used to identify genotypes in the epidemiological study of HAdVs (34)(35)(36)(37)(38)(39)(40)(41)(42). This method can divide genotypes that cannot be differentiated by sequence analysis of the hexon gene (35).…”