Detection and Serotyping of Human Adenoviruses from Patients with Influenza-Like Illness in Mongolia

Tohma, Kentaro; Bayasgalan, Namuuntsetsegiin; Suzuki, Akira; Darma, Badarchiin; Oshitani, Hitoshi; Nymadawa, Pagbajabyn

doi:10.7883/yoken.65.289

Cited by 6 publications

(3 citation statements)

References 34 publications

(43 reference statements)

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“…Some substitutions in the HAdV amino acids sequences were observed and analyzed in this work. Other recent studies have observed substitutions of amino acids in respiratory HAdV, suggesting that the substitutions are related to respiratory-tissue viral tropism [38,39].…”

Section: Discussionmentioning

confidence: 85%

Evaluation and molecular characterization of human adenovirus in drinking water supplies: viral integrity and viability assays

Fongaro

Nascimento

Rigotto

et al. 2013

Virol J

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BackgroundHuman adenoviruses (HAdVs) are the second-leading cause of childhood gastroenteritis worldwide. This virus is commonly found in environmental waters and is very resistant to water disinfection and environmental stressors, especially UV light inactivation. Molecular techniques, such as PCR-based methods (Polymerase Chain Reaction), are commonly used to detect and identify viral contamination in water, although PCR alone does not allow the discrimination between infectious and non-infectious viral particles. A combination of cell culture and PCR has allowed detection of infectious viruses that grow slowly or fail to produce cytopathic effects (CPE) in cell culture. This study aimed to assess the integrity and viability of human adenovirus (HAdV) in environmental water and evaluate circulating strains by molecular characterization in three sites of the water supply in Florianópolis, Santa Catarina Island, Brazil: Peri Lagoon water, spring source water, and water from the public water supply system.MethodsWater samples were collected, concentrated and HAdV quantified by real-time PCR. Viral integrity was evaluated by enzymatic assay (DNase I) and infectivity by plaque assay (PA) and integrated cell culture using transcribed mRNA (ICC-RT-qPCR). Samples containing particles of infectious HAdV were selected for sequencing and molecular characterization.ResultsThe analyzed sites contained 83, 66 and 58% undamaged HAdV particles (defined as those in which the genetic material is protected by the viral capsid) at Peri Lagoon, spring source water and public supply system water, respectively. Of these, 66% of the particles (by PA) and 75% (by ICC-RT-qPCR) HAdV were shown to be infectious, due to being undamaged in Peri Lagoon, 33% (by PA) and 58% (by ICC-RT-qPCR) in spring source water and 8% (by PA) and 25% (by ICC-RT-qPCR) in the public water supply system. ICC-RT-qPCR, a very sensitive and rapid technique, was able to detect as low as 1 × 102 HAdV genome copies per milliliter of infectious viral particles in the environmental water samples. The molecular characterization studies indicated that HAdV-2 was the prevalent serotype.ConclusionsThese results indicate a lack of proper public health measures. We suggest that HAdV can be efficiently used as a marker of environmental and drinking water contamination and ICC-RT-qPCR demonstrated greater sensitivity and speed of detection of infectious viral particles compared to PA.

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Section: Discussionmentioning

confidence: 85%

Evaluation and molecular characterization of human adenovirus in drinking water supplies: viral integrity and viability assays

Fongaro

Nascimento

Rigotto

et al. 2013

Virol J

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“…Symptoms caused by HAdV infection range from mild symptoms to severe pneumonia. Furthermore, respiratory illness associated with HAdV can be confused with an influenza‐like illness (ILI) including fever, coughing, sore throat, and muscle pain [Tohma et al, ]. To avoid the misdiagnosis of HAdV infection from patients with an ILI, a molecular biology technique such as direct PCR is used for discriminating possible causative viral agents.…”

Section: Discussionmentioning

confidence: 99%

Molecular epidemiology of a post-influenza pandemic outbreak of acute respiratory infections in Korea caused by human adenovirus type 3

et al. 2014

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An outbreak of upper respiratory tract infections associated with human adenovirus (HAdV) occurred on a national scale in Korea from September to December 2010, following a major H1N1 influenza pandemic. Data from the Korea Influenza and Respiratory Surveillance System (KINRESS) showed an unusually high positive rate accounting for up to 20% of all diagnosed cases. To determine the principal cause of the outbreak, direct polymerase chain reaction (PCR) amplification followed by sequence analysis targeting parts of the hexon gene of HAdV was performed. Serotypes of 1,007 PCR-diagnosed HAdV-positive samples from patients with an acute upper respiratory tract illness were determined and epidemiological characteristics including major aged group and clinical symptoms were analyzed. The principal symptom of HAdV infections was fever and the vulnerable aged group was 1-5 years old. Based on sequence analysis, HAdV-3 was the predominant serotype in the outbreak, with an incidence of 74.3%. From the beginning of 2010 until May, the major serotypes were HAdV-1, 2, and 5 (70-100%) in any given period. However, an outbreak dominated by HAdV-3 started between July and August and peaked in September. Phylogenetic analysis revealed that there was no genetic variation in HAdV-3. The results demonstrated that an outbreak of upper respiratory illness followed by H1N1 influenza pandemic in Korea was caused mainly by emerged HAdV-3. J.

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“…Under these species, 104 adenovirus genotypes have been identified (HadV Working Group http://hadvwg.gmu.edu, ICTV 2011). Human adenoviruses (HAdV) cause variable serotype-specific diseases (Tohma et al, 2012). Genotypes under Human mastadenovirus B and E have been associated with epidemics of severe acute respiratory infection affecting children and adults (Hong et al, 2001b).…”

Section: Introductionmentioning

confidence: 99%

Serotype Identification of Human Adenoviruses Associated with Influenza-Like Illnesses in the Philippines from 2006–2012 by Microneutralization and Molecular Techniques

Calzado

Foronda

Arguelles

et al. 2022

International Journal of Infectious Diseases

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Detection and Serotyping of Human Adenoviruses from Patients with Influenza-Like Illness in Mongolia

Cited by 6 publications

References 34 publications

Evaluation and molecular characterization of human adenovirus in drinking water supplies: viral integrity and viability assays

Evaluation and molecular characterization of human adenovirus in drinking water supplies: viral integrity and viability assays

Molecular epidemiology of a post-influenza pandemic outbreak of acute respiratory infections in Korea caused by human adenovirus type 3

Serotype Identification of Human Adenoviruses Associated with Influenza-Like Illnesses in the Philippines from 2006–2012 by Microneutralization and Molecular Techniques

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