Genetic basis of the highly efficient yeast Kluyveromyces marxianus: complete genome sequence and transcriptome analyses

Lertwattanasakul, Noppon; Kosaka, Tomoyuki; Hosoyama, Akira; Suzuki, Yutaka; Rodrussamee, Nadchanok; Matsutani, Minenosuke; Murata, Masayuki; Fujimoto, Naoko; Suprayogi, Suprayogi; Tsuchikane, Keiko; Limtong, Savitree; Fujita, Nobuyuki; Yamada, Mamoru

doi:10.1186/s13068-015-0227-x

Cited by 151 publications

(171 citation statements)

References 68 publications

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“…In the case of unconventional thermotolerant yeast K. marxianus, however, there were only several reports of transcriptome analysis on yeast responses from different carbon source including glucose, inulin and xylose, or the tolerance to high temperature or ethanol stress. 18,69,70,71 To the best of our knowledge, genome-wide transcription analysis under the multiple inhibitors stress condition with elevated temperature (42 C) has not been reported for this yeast.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic analysis of thermotolerant yeastKluyveromyces marxianusin multiple inhibitors tolerance

Wang

Yang

et al. 2018

RSC Adv.

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During pretreatment of lignocellulosic biomass, toxic compounds were released and inhibited the growth and fermentation of microorganisms. Here the global transcriptional response of K. marxianus to multiple inhibitors including acetic acid, phenols, furfural and HMF, at 42 C, was studied, via RNA-seq technology.Genes involved in the glycolysis pathway, fatty acid metabolism, ergosterol metabolism and vitamin B6 and B1 metabolic process were enriched in the down-regulated gene set, while genes involved in TCA cycle, respiratory chain, ROS detoxification and transporter coding genes were enriched in the up-regulated gene set in response to the multiple inhibitors stress. Further real time-PCR results with three single inhibitor stress conditions showed that different transporters responded quite differently to different inhibitor stress. Coenzyme assay results showed that the level of NAD + was increased and both NADH/ NAD + and NADPH/NADP + ratio decreased. Furthermore, genes involved with transcription factors related to carbohydrate metabolism, sulfur amino acids metabolism, lipid metabolism or those directly involved in the transcriptional process were significantly regulated. Though belonging to different GO categories or KEGG pathway, many differentially expressed genes were enriched in maintaining the redox balance, NAD(P) + /NAD(P)H homeostasis or NAD + synthesis, energy production, and iron transportation or metabolism. These results suggest that engineering these aspects represents a possible strategy to develop more robust strains for industrial fermentation from cellulosic biomass.

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Section: Discussionmentioning

confidence: 99%

Transcriptomic analysis of thermotolerant yeastKluyveromyces marxianusin multiple inhibitors tolerance

Wang

Yang

et al. 2018

RSC Adv.

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“…K. marxianus has number of advantages over S. cerevisiae, including fermentation of various sugars at high temperatures, weak glucose repression and fermentability of inulin. However, its fermentation activity from xylose by K. marxianus is low compared to that of glucose [27,28].…”

Section: Analysis Of Algal Biomass Compositionmentioning

confidence: 95%

Ethanol production from brown seaweed using non-conventional yeasts

Obata¹,

Akunna²,

Bockhorn³

et al. 2016

Bioethanol

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Abstract:The use of macroalgae (seaweed) as a potential source of biofuels has attracted considerable worldwide interest. Since brown algae, especially the giant kelp, grow very rapidly and contain considerable amounts of polysaccharides, coupled with low lignin content, they represent attractive candidates for bioconversion to ethanol through yeast fermentation processes. In the current study, powdered dried seaweeds (Ascophylum nodosum and Laminaria digitata) were pre-treated with dilute sulphuric acid and hydrolysed with commercially available enzymes to liberate fermentable sugars. Higher sugar concentrations were obtained from L. digitata compared with A. nodosum with glucose and rhamnose being the predominant sugars, respectively, liberated from these seaweeds. Fermentation of the resultant seaweed sugars was performed using two non-conventional yeast strains: Scheffersomyces (Pichia) stipitis and Kluyveromyces marxianus based on their abilities to utilise a wide range of sugars. Although the yields of ethanol were quite low (at around 6 g/L), macroalgal ethanol production was slightly higher using K. marxianus compared with S. stipitis. The results obtained demonstrate the feasibility of obtaining ethanol from brown algae using relatively straightforward bioprocess technology, together with non-conventional yeasts. Conversion efficiency of these non-conventional yeasts could be maximised by operating the fermentation process based on the physiological requirements of the yeasts.

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“…In the thermotolerant yeast K. marxianus, the molecular mechanisms conferring thermotolerance are complicated and are controlled by multiple genes not only for HSPs biosynthesis but also for those genes encoding the proteins involved in DNA replication and repair, RNA processing, ribosome biogenesis, and carbohydrate metabolism process [21]. The expression of hsp genes and those functioning to prevent protein denaturation may be insufficient to allow growth and efficient ethanol fermentation of S. cerevisiae at high temperatures.…”

Section: Real-time Rt-pcr Analysis Of Gene Expressionmentioning

confidence: 99%

Ethanol Production from Sweet Sorghum Juice at High Temperatures Using a Newly Isolated Thermotolerant Yeast Saccharomyces cerevisiae DBKKU Y-53

et al. 2016

Self Cite

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Ethanol production at elevated temperatures requires high potential thermotolerant ethanol-producing yeast. In this study, nine isolates of thermotolerant yeasts capable of growth and ethanol production at high temperatures were successfully isolated. Among these isolates, the newly isolated thermotolerant yeast strain, which was designated as Saccharomyces cerevisiae DBKKU Y-53, exhibited great potential for ethanol production from sweet sorghum juice (SSJ) at high temperatures. The maximum ethanol concentrations produced by this newly isolated thermotolerant yeast at 37˝C and 40˝C under the optimum cultural condition were 106.82 g¨L´1 and 85.01 g¨L´1, respectively, which are greater than values reported in the literatures. It should be noted from this study with SSJ at a sugar concentration of 250 g¨L´1 and an initial pH of 5.5 without nitrogen supplementation can be used directly as substrate for ethanol production at high temperatures by thermotolerant yeast S. cerevisiae DBKKU Y-53. Gene expression analysis using real-time RT-PCR clearly indicated that growth and ethanol fermentation activities of the thermotolerant yeast S. cerevisiae DBKKU Y-53 at a high temperature (40˝C) were not only restricted to the expression of genes involved in the heat-shock response, but also to those genes involved in ATP production, trehalose and glycogen metabolism, and protein degradation processes were also involved.

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Genetic basis of the highly efficient yeast Kluyveromyces marxianus: complete genome sequence and transcriptome analyses

Cited by 151 publications

References 68 publications

Transcriptomic analysis of thermotolerant yeastKluyveromyces marxianusin multiple inhibitors tolerance

Transcriptomic analysis of thermotolerant yeastKluyveromyces marxianusin multiple inhibitors tolerance

Ethanol production from brown seaweed using non-conventional yeasts

Ethanol Production from Sweet Sorghum Juice at High Temperatures Using a Newly Isolated Thermotolerant Yeast Saccharomyces cerevisiae DBKKU Y-53

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