Ethanol Production from Sweet Sorghum Juice at High Temperatures Using a Newly Isolated Thermotolerant Yeast Saccharomyces cerevisiae DBKKU Y-53

Nuanpeng, Sunan; Thanonkeo, Sudarat; Yamada, Mamoru; Thanonkeo, Pornthap

doi:10.3390/en9040253

Cited by 46 publications

(72 citation statements)

References 71 publications

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“…In this study, SSJ supplemented only with (NH 4 ) 2 SO 4 was directly used by S. cerevisiae KKU-VN8 to produce ethanol, which differs from other conditions reported in the literature 3, 15, 24, 25, 26, 27, 28, 30, 66Table 6Comparison of ethanol production by S. cerevisiae KKU-VN8 and other yeast strains reported in the literatures.Strain S 0 (g/L)Sugar source T (°C) P (g/L)SC(%)T.Y.(%)Reference K. marxianus DMKU 3-1042220Sugar cane juice4067.8N/A60.4Limtong et al 3 I. orientalis IPE 100150Glucose4265.5N/A85.5Kwon et al 17 P. kudriavzevii DMKU 3-ET15180Cassava starch hydrolysate4078.6N/A85.5Yuangsaard et al 15 S. cerevisiae NRRL Y-2034200Sweet sorghum juice3070.6N/AN/APhutela and Kaur 67 S. cerevisiae AT-3180Glucose4068.590.075.0Tofighi et al 66 S. cerevisiae SEMF1185Sweet sorghum juice3386.297.391.9Zhao et al 28 S. cerevisiae DBKKU Y-53<...>…”

Section: Discussionmentioning

confidence: 92%

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High-temperature ethanol production using thermotolerant yeast newly isolated from Greater Mekong Subregion

Techaparin

Thanonkeo

Klanrit

2017

Brazilian Journal of Microbiology

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The application of high-potential thermotolerant yeasts is a key factor for successful ethanol production at high temperatures. Two hundred and thirty-four yeast isolates from Greater Mekong Subregion (GMS) countries, i.e., Thailand, The Lao People's Democratic Republic (Lao PDR) and Vietnam were obtained. Five thermotolerant yeasts, designated Saccharomyces cerevisiae KKU-VN8, KKU-VN20, and KKU-VN27, Pichia kudriavzevii KKU-TH33 and P. kudriavzevii KKU-TH43, demonstrated high temperature and ethanol tolerance levels up to 45 °C and 13% (v/v), respectively. All five strains produced higher ethanol concentrations and exhibited greater productivities and yields than the industrial strain S. cerevisiae TISTR5606 during high-temperature fermentation at 40 °C and 43 °C. S. cerevisiae KKU-VN8 demonstrated the best performance for ethanol production from glucose at 37 °C with an ethanol concentration of 72.69 g/L, a productivity of 1.59 g/L/h and a theoretical ethanol yield of 86.27%. The optimal conditions for ethanol production of S. cerevisiae KKU-VN8 from sweet sorghum juice (SSJ) at 40 °C were achieved using the Box–Behnken experimental design (BBD). The maximal ethanol concentration obtained during fermentation was 89.32 g/L, with a productivity of 2.48 g/L/h and a theoretical ethanol yield of 96.32%. Thus, the newly isolated thermotolerant S. cerevisiae KKU-VN8 exhibits a great potential for commercial-scale ethanol production in the future.

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Section: Discussionmentioning

confidence: 92%

“…Furthermore, optimal conditions are determined with a second-order polynomial equation 29 . Many factors, including incubation temperature, initial yeast cell concentration, initial pH and sugar concentration, affect the growth and ethanol production of yeast cells 3, 11, 26, 30…”

Section: Introductionmentioning

confidence: 99%

High-temperature ethanol production using thermotolerant yeast newly isolated from Greater Mekong Subregion

Techaparin

Thanonkeo

Klanrit

2017

Brazilian Journal of Microbiology

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“…Auesukaree et al [51] reported a strain C3867 that produced 38.8 g/L of ethanol at 41°C. Recently, Nuanpeng et al [52] and Techaparin et al [53] isolated S. cerevisiae DBKKUY-53 and KKU-VN8, respectively, in Thailand. The former strain produced the maximum ethanol concentration and volumetric ethanol productivity of 85.0 g/L and 2.83 g/L h, respectively, at 40°C, and the latter strain produced the maximum ethanol concentration of 89.3 g/L with a productivity of 2.48 g/L h and a theoretical ethanol yield of 96.3% from sweet sorghum juice at 40°C.…”

Section: Various Ethanologenic Thermotolerant Yeasts and Their Characmentioning

confidence: 99%

Potential of Thermotolerant Ethanologenic Yeasts Isolated from ASEAN Countries and Their Application in High- Temperature Fermentation

Kosaka¹,

Lertwattanasakul²,

NadchanokRodrussamee³

et al. 2019

Fuel Ethanol Production From Sugarcane

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Thermotolerant ethanologenic yeasts receive attention as alternative bio-ethanol producers to traditionally used yeast, Saccharomyces cerevisiae. Their utilization is expected to provide several benefits for bio-ethanol production due to their characteristics and robustness. They have been isolated from a wide variety of environments in a number of ASEAN countries: Thailand, Vietnam, Laos, and Indonesia. One of these yeasts, Kluyveromyces marxianus has been investigated regarding characteristics. Some strains efficiently utilize xylose, which is a main component of the 2nd generation biomass. In addition, the genetic basis of K. marxianus has been revealed by genomic sequencing and is exploited for further improvement of the strains by thermal adaptation or gene engineering techniques. Moreover, the glucose repression of K. marxianus and its mechanisms has been investigated. Results suggest that K. marxianus is an alternative to S. cerevisiae in next-generation bio-ethanol production industry. Indeed, we have succeeded to apply K. marxianus for bio-ethanol production in a newly developed process, which combines high-temperature fermentation with simultaneous fermentation and distillation under low pressure. This chapter aims to provide valuable information on thermotolerant ethanologenic yeasts and their application, which may direct the economic bioproduction of ethanol and other useful materials in the future.Recently, thermotolerant microorganisms were found among mesophiles with optimum growth temperatures that are 5-10°C higher than those of the typical mesophilic strains Fuel Ethanol Production from Sugarcane 122 Potential of Thermotolerant Ethanologenic Yeasts Isolated from ASEAN Countries… http://dx.doi.org/10.5772/intechopen.79144 123Recently, there have been several reports on ethanol production at high temperatures using P. kudriavzevii (formerly known as I. orientalis). Several P. kudriavzevii strains were reported to grow and produce high levels of ethanol at high temperatures. The strain DMKU 3-ET15 was isolated from traditional fermented pork sausage in Thailand by an enrichment technique in a medium supplemented with 4% ethanol at 40°C. The strain produced 78.6 g/L ethanol from 180 g/L glucose at 40°C [20]. The strain KVMP10 that was isolated from soil located beneath apple trees for ethanol production from orange peel achieved 54 g/L ethanol at 42°C [48]. Strain RZ8-1 that was recently isolated from various samples collected from plant orchards in Thailand produced 33.8 g/L ethanol from 160 g/L glucose at 40°C [49].

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“…Ethanol tolerances also are known to be temperature subordinate [35]. Earlier researches have either centered on hereditary variety in either ethanol or temperature push however not both [42] [43] [44]. The two crosses contrasted in the other parent (YJF153 or SD1) and the impacts of the sensitive SEC24 and PSD1 alleles from HN6.…”

Section: Tropicalis Ctropicalis Tribeigelii Tribeigelii Blastcapimentioning

confidence: 99%

Selection of Mozzarella Cheese Whey Native Yeasts with Ethanol and Glucose Tolerance Ability

Balia

Kurnani

Utama

2018

International Journal on Advanced Science, Engineering and Information Technology

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The research aimed to determine the native yeast on mozzarella cheese whey that has glucose and ethanol tolerance ability. The research did experimentally and the data analyzed descriptively. Native yeasts isolated from 1 ml mozzarella cheese whey with using a modification of Potato Dextrose Agar/PDA (Oxoid Ltd.) with the addition of 3% Yeasts Extract/YE (Kraft Foods) and 10 ppm amoxicillin. The yeasts identified for macroscopic and microscopic characteristics then tested with RapID Yeast Plus System. The ability in tolerate ethanol and glucose contents tested by grown the yeasts on modified Nutrient Broth/NB (Oxoid Ltd.) with 3% Yeasts Extract/YE (Kraft Foods) and 10 ppm amoxicillin then added with glucose monohydrates (10%, 20%, 30%) or ethanol (10%, 20%, 30%) and incubated for 72h at room temperature (23-28°C). Optical density (OD) read for UV absorbance at 600 nm using UV-Vis spectrophotometer every 24h until 72h. Results showed that six native yeasts isolated and identified as C. tropicalis three isolate, Tri. beigelii two isolates and Blast. capitatus is one isolate. The best isolates with highest OD at 30% glucose concentration (2.215) gained by C.tropicalis (a), while the highest OD at 30% ethanol concentration (0.508) shown by C.tropicalis (f).

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Ethanol Production from Sweet Sorghum Juice at High Temperatures Using a Newly Isolated Thermotolerant Yeast Saccharomyces cerevisiae DBKKU Y-53

Cited by 46 publications

References 71 publications

High-temperature ethanol production using thermotolerant yeast newly isolated from Greater Mekong Subregion

High-temperature ethanol production using thermotolerant yeast newly isolated from Greater Mekong Subregion

Potential of Thermotolerant Ethanologenic Yeasts Isolated from ASEAN Countries and Their Application in High- Temperature Fermentation

Selection of Mozzarella Cheese Whey Native Yeasts with Ethanol and Glucose Tolerance Ability

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