Genetic and phenotypic diversity of plant-growth-promoting bacilli isolated from wheat fields in southern Brazil

Beneduzi, Anelise; Peres, Daiane; Costa, Pedro Beschoren da; Zanettini, Maria Helena Bodanese; Passaglia, Luciane Maria Pereira

doi:10.1016/j.resmic.2008.03.003

Cited by 92 publications

(55 citation statements)

References 28 publications

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“…The production of the phytohormone (IAA) and siderophores by Bacillus species has been investigated in many studies. The IAA stimulates the plant growth and siderophores chelate iron (Fe) and deprive the phytopathogenic fungi of it [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24]. Here, the Bacillus spp.…”

Section: Resultsmentioning

confidence: 99%

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Biocontrol and Plant Growth Promotion Characterization of Bacillus Species Isolated from Calendula officinalis Rhizosphere

et al. 2013

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The phenotypic and genotypic diversity of the plant growth promoting Bacillus genus have been widely investigated in the rhizosphere of various agricultural crops. However, to our knowledge this is the first report on the Bacillus species isolated from the rhizosphere of Calendula officinalis. 15 % of the isolated bacteria were screened for their important antifungal activity against Fusarium oxysporum, Botrytis cinerea, Aspergillus niger, Cladosporium cucumerinium and Alternaria alternata. The bacteria identification based on 16S r-RNA and gyrase-A genes analysis, revealed strains closely related to Bacillus amyloliquefaciens, B. velezensis, B. subtilis sub sp spizezenii and Paenibacillus polymyxa species. The electro-spray mass spectrometry coupled to liquid chromatography (ESI-LC MS) analysis showed that most of the Bacillus isolates produced the three lipopeptides families. However, the P. polymyxa (18SRTS) didn't produce any type of lipopeptides. All the tested Bacillus isolates produced cellulase but the protease activity was observed only in the B. amyloliquefaciens species (9SRTS). The Salkowsky colorimetric test showed that the screened bacteria synthesized 6-52 lg/ml of indole 3 acetic acid. These bacteria produced siderophores with more than 10 mm wide orange zones on chromazurol S. The greenhouse experiment using a naturally infested soil with Sclerotonia sclerotiorum showed that the B. amyloliquefaciens (9SRTS) had no significant (P [ 0.05) effect on the pre-germination of the chickpea seeds. However, it increased the size of the chickpea plants and reduced the stem rot disease (P \ 0.05).These results suggested that the Bacillus strains isolated in this work may be further used as bioinoculants to improve the production of C. officinalis and other crop systems.

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Section: Resultsmentioning

confidence: 99%

“…The indole acetic acid production was assayed calorimetrically by using the Salkowski reagent (0.01 M FeCl 3 in 36 % H 2 SO 4 ) as described by Benduzi et al [5]. The test was achieved in duplicate.…”

Section: Production Of Indole 3 Acetic Acid (Iaa)mentioning

confidence: 99%

Biocontrol and Plant Growth Promotion Characterization of Bacillus Species Isolated from Calendula officinalis Rhizosphere

et al. 2013

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“…It has been previously isolated from the rhizosphere of maize [38] and wheat [39] and has been also found as an endophyte of Eucalyptus, soybean, and Phaseolus vulgaris beans [31,33,40].…”

Section: Discussionmentioning

confidence: 99%

Analysis of the Bacterial Diversity Associated with the Roots of Maize (Zea mays L.) through Culture-Dependent and Culture-Independent Methods

et al. 2011

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The present study investigated bacterial diversity associated with the roots of maize through the use of culture-dependent and culture-independent methods. Bacterial 16S-23S rDNA internal transcribed spacer sequences (ITS) primers were used to amplify sequences obtained directly from the root matrix by Percoll gradient separation. This assay showed that γ-Proteobacteria within Enterobacter, Erwinia, Klebsiella, Pseudomonas, and Stenotrophomonas genera were predominant groups. The culturable component of the bacterial community was also assessed, revealing that the predominant group was Firmicutes, mainly of Bacillus genus, while Achromobacter, Lysinibacillus, and Paenibacillus genera were rarely found in association with the roots. Only two genera within γ-Proteobacteria, Enterobacter and Pseudomonas, were found in the culture collection. Differences in richness and diversity between the rhizospheric and endophytic bacterial communities were also evidenced. The spectrum of bacteria naturally associated with maize roots is wide and the magnitude of such diversity will depend on the methods chosen for analysis. The knowledge of this spectrum will facilitate the search of microorganisms capable of exerting antagonism to diverse pathogens or detecting plant growth enhancers.

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“…Verma et al (2013) reported that the plant growth-promoting microbes have greater ability for ammonia production, which is isolated from rhizosphere soils. Recently, Beneduzi et al (2008) advocated that the Bacillus and Paenibacillus have the ability of nitrogen fixation, synthesize IAA and solubilize phosphates which promote the growth of rice crops. In last, isolated microbial strains IESDV2, IESDV3, IESDV4, IESDV11, IESDV12, IESDV26, IESDV28 were recorded significant increase in the production of IAA, ammonia and phosphate solubilization as compared to others.…”

Section: Plant Growth-promoting Activities Of Isolated Strainsmentioning

confidence: 99%

Screening of bacterial strains for developing effective pesticide-tolerant plant growth-promoting microbial consortia from rhizosphere soils of vegetable fields of eastern Uttar Pradesh, India

Verma

Jaiswal

Maurya

2016

Energ. Ecol. Environ.

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The current study was intended to isolate and characterize the plant growth-promoting properties and pesticide (chlorpyripfos) tolerance ability of microbial strains for sustainable vegetable productions. Plant growthpromoting microorganism is a group of microbial consortia to improve crop growth and yield by various direct mechanisms, e.g. nitrogen fixation, phosphate solubilization, production of plant growth hormones, ammonia and siderophore, and indirect mechanisms, e.g. production of antibiotic, hydrogen cyanide to help as biocontrolling agent. Totally, 50 microbes were isolated from soils of vegetable field. Out of 50, 14 strains were selected on the basis of morphological, growth biochemically characters (e.g. gram staining, amylase, cellulase, catalase and citrate test) and plant growth-promoting properties. All strains have ability of ammonia production. Out of 14 strains, IESDV2, IESDV3, IESDV4, IESDV11, IESDV12 and IESD28 were found significant increase in production of IAA, ammonia and phosphate solubilization than others. Strains IESDV3 and IESDV11 showed tolerant at 6 ll/ml concentration of chlorpyrifos which is three-time increase concentration of recommended dose (2 ll/ml). Others strains IESDV12, IESDV13 and IESDV28 were also showed resistance or tolerance at different concentrations of pesticide 2, 4, 6 and 20 ll/ml. These strains also showed plant growth-promoting rhizobacteria activities. Therefore, the main result of this study is to screen and identify that three strains (IESDV3, IESDV11, IESDV12 and IESDV28) can be used as effective pesticide-tolerant plant growth-promoting microbial consortia for vegetable production under sustainable agriculture at eastern Uttar Pradesh.

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Genetic and phenotypic diversity of plant-growth-promoting bacilli isolated from wheat fields in southern Brazil

Cited by 92 publications

References 28 publications

Biocontrol and Plant Growth Promotion Characterization of Bacillus Species Isolated from Calendula officinalis Rhizosphere

Biocontrol and Plant Growth Promotion Characterization of Bacillus Species Isolated from Calendula officinalis Rhizosphere

Analysis of the Bacterial Diversity Associated with the Roots of Maize (Zea mays L.) through Culture-Dependent and Culture-Independent Methods

Screening of bacterial strains for developing effective pesticide-tolerant plant growth-promoting microbial consortia from rhizosphere soils of vegetable fields of eastern Uttar Pradesh, India

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