Genetic analysis of the first mcr-1 positive Escherichia coli isolate collected from an outpatient in Chile

Gutiérrez, Camila Fernández; Zenis, Javier; Legarraga, Paulette; Cabrera‐Pardo, Jaime R.; García, Patricia; Bello-Toledo, Helia; Opazo-Capurro, Andrés; González-Rocha, Gerardo

doi:10.1016/j.bjid.2019.05.008

Cited by 13 publications

(6 citation statements)

References 22 publications

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“…Likewise, mcr-1 has been identified in isolates from humans, animals, and the environment in an increasing number of countries [25] namely, Asia, Europe, North America, Africa, and the Middle East [26,27]. To date, mcr-1 has been observed on a wide variety of plasmid types, including IncI2, IncHI2, IncX4 [28], IncP, IncFI, IncFIB [29] and IncHI1, IncFII, IncX1-X2, IncX3 [30]. Moreover, retrospective studies led to establishing the presence of the mcr-1 gene in China as early as the 1980s and in Europe since 2005 [31,32].…”

Section: Mcr Gene Associated With Resistance To Colistinmentioning

confidence: 99%

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mcr-mediated colistin resistance in South America, a One Health approach: a review

Daza-Cardona

Buenhombre

Fontenelle

et al. 2021

Reviews in Medical Microbiology

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Antimicrobial resistance is recognized as one of the most important global human health problems in the 21st century. The balance between clinical need and resistance prevention is further compromised by agricultural antibiotic use as some countries have actively used colistin in animal production. Antimicrobial-resistant microorganisms have been found in humans, food, animals, plants, and the environment (water, soil, and air), and they can move between ecosystems. The problem of antimicrobial resistance is currently seen in the concept: One Health. This approach encourages interdisciplinary collaborative efforts that work locally. The beginnings of the mcr gene's presence in America dates to 2012 and the first report of disseminating the mcr-1 gene was described for Kelp gulls (Larus dominicanus) from Ushuaia, Argentina. It can be associated with the possible dissemination of the gene in the American continent through migratory birds. The mcr-1 gene is disseminated in countries, such as Argentina,

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Section: Mcr Gene Associated With Resistance To Colistinmentioning

confidence: 99%

“…Thirteen human clinical samples were analyzed with only one positive isolate for the mcr-1 gene in 2018 [93]. The isolated carried the bla CTX-M-55, and the gene mcr-1 was detected in an IncI2-plasmid, which is known to be promiscuous [30]. As far as we have known, there are no additional reports.…”

Section: Chilementioning

confidence: 99%

mcr-mediated colistin resistance in South America, a One Health approach: a review

Daza-Cardona

Buenhombre

Fontenelle

et al. 2021

Reviews in Medical Microbiology

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“…HGT of mcr -1 is a threat to human health, so it is necessary to calculate the efficiency of mcr -1 transfer. The reported transfer efficiency of mcr -1 varies greatly depending on the experimental conditions, methods, and strains, ranging from 10 –9 to 10 –1 ( Anjum et al, 2016 ; Liu et al, 2016 ; Quesada et al, 2016 ; Gutiérrez et al, 2019 ; Lu et al, 2019 ; Li et al, 2022 ). In our study, mcr -1 from all five colistin-resistant strains was successfully transferred into the E. coli J53 recipient strain by conjugation.…”

Section: Discussionmentioning

confidence: 99%

Rapid Detection of Multi-Resistance Strains Carrying mcr-1 Gene Using Recombinase-Aided Amplification Directly on Clinical Samples

Fan

Feng

et al. 2022

Front. Microbiol.

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With the increasingly severe problem of bacterial resistance, colistin, as the last line of defense, has attracted attention again. Mobile colistin resistance (mcr-1) gene is involved in the horizontal transmission of colistin resistance in Gram-negative bacteria (GNB), which is a serious threat to human health. Therefore, rapid detection of mcr-1 gene presence in clinical samples is crucial. In this study, a Recombinase-aided amplification(RAA) method for mcr-1 was successfully constructed, with sensitivity of 20 copies/reaction. In addition, amplification signal could only be detected in the strain containing mcr-1 gene among 14 different bacterial species. The method was then used to test a total of 672 clinical samples from a pediatric hospital in Beijing. Five strains harbored mcr-1 genes were isolated from mcr-1-positive clinical samples and identified as Escherichia coli. Multi-locus sequence typing (MLST) analysis showed that the five E. coli belonged to different ST types. Notably, the mcr-1 gene from the isolates could be transferred conjugately to the recipient strain E. coli J53, with highest transfer efficiency up to 57–58%, suggesting that the mcr-1 gene was located on the plasmid. These findings showed that the RAA assay has potential to be a rapid and sensitive mcr-1 gene screening test for clinical samples, and mcr-1 could be transmitted vertically and horizontally between and within bacterial species in a plasmid-mediated manner.

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“…This could be attributed to the fact that until the first report of mcr-1 gene, colistin resistance in Enterobacteriaceae was believed to be chromosomally mediated 36 . However, since 2016, the mcr-1 gene was reported in Enterobacteriaceae recovered from food, animals, and human specimens in China, and it has subsequently been reported worldwide 11, [48][49][50][51][52][53] . The wide spread of the mcr-1 dramatically challenges the newly renewed interest in colistin for clinical use and opens a new research topic on colistin.…”

Section: Discussionmentioning

confidence: 99%

Global trends and current status in colistin resistance research: a bibliometric analysis (1973-2019)

Yacouba

Olowo-Okere

2020

F1000Res

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Background: Colistin resistance is a major breach in our last line of defense and without urgent action, we are heading for a post-antibiotic era, in which common infections and minor injuries can once again kill. To the best of our knowledge, the use of the bibliometric analytical technique for examining colistin resistance-related research does not exist in the literature. Methods: Here, we analyze and present bibliometric indicators of the global literature in colistin resistance research. The Scopus database was searched for articles on colistin resistance. The articles retrieved were analyzed using the bibliometrix R-package. Results: A total of 1105 publications were retrieved. There was a noticeable increase in the number of publications on colistin resistance research in the past decade. Six journals made up the core zone in colistin research and produced 35.83% of the published articles. The analysis across time-intervals revealed several keywords that had increased or decreased in usage when comparing the interval between 1973-2009 and 2010-2019. Authors’ keywords “Acinetobacter baumanii”, and “Pseudomonas aeruginosa” were the most frequent encountered during the period of 1973-2009, while “mcr-1”, “Enterobacteriaceae”, “Escherichia coli”, and “Klebsiella pneumoniae” emerged in the past decade. Conclusions: There has been a significant growth in publications on colistin resistance in the past decade, suggesting an urgent need for action by different stakeholders to contain this threat of colistin resistance. Keyword analysis revealed temporal changes in the types of keywords used across time-intervals. These findings summarize a general vision on colistin resistance research and will serve as baseline data for future comparative purposes.

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Genetic analysis of the first mcr-1 positive Escherichia coli isolate collected from an outpatient in Chile

Cited by 13 publications

References 22 publications

mcr-mediated colistin resistance in South America, a One Health approach: a review

mcr-mediated colistin resistance in South America, a One Health approach: a review

Rapid Detection of Multi-Resistance Strains Carrying mcr-1 Gene Using Recombinase-Aided Amplification Directly on Clinical Samples

Global trends and current status in colistin resistance research: a bibliometric analysis (1973-2019)

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