Genetic analysis of the 9.5-kilobase virulence plasmid of Yersinia pestis

Sodeinde, Olanrewaju A.; Goguen, Jon D.

doi:10.1128/iai.56.10.2743-2748.1988

Cited by 140 publications

(83 citation statements)

References 30 publications

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“…This fragment contains the replication region of plBI and is highly conserved in Yersinia virulence piasmids (Biot and Cornelis, 1987). From previous studies, it is known that the replication area harbours two other piasmids in addition to pCDI, a 9.5 kb piasmid and a cryptic c. 110kb piasmid (Sodeinde and Goguen, 1988). The maps of pYV989 (V. enterocolitica 0:9) and of pYV654 (K enterocolitica 0:3) show very similar patterns to those of earlier published maps of Y. enterocolitica of the serogroups 0:9 and 0:3 (Laroche ef ai.…”

supporting

confidence: 76%

Homologous DNA sequences on the virulence plasmids of pathogenic Yersinia and Salmonella dublin Lane

Krause

Fierer

Guiney

1990

Molecular Microbiology

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Yersinia and Salmonella harbour plasmids that encode traits important for virulence, enabling both pathogenic genera to survive and grow in cells of the reticulo-endothelial organs during systemic infections. We have detected DNA homology between the Salmonella dublin virulence plasmid pSDL2 and the plasmids of the pathogenic Yersinia species pestis, pseudotuberculosis, and enterocolitica. Three regions of pSDL2 were found to share homology with the virulence plasmid pIB1 of Yersinia pseudotuberculosis. Two separate hybridizing segments mapped within the previously characterized 6.4 kb vir region of pSDL2 in the SalI B fragment. The third homologous region involved the replicon of pIB1, which hybridized to the SalI C2 fragment of pSDL2. The virulence plasmid pCD1 from Y. pestis showed similar homology with the three regions of pSDL2. Homologies to the vir and SalI C2 regions of pSDL2 were also found on plasmids from Yersinia enterocolitica serotypes 0:9, 0:3 and 0:5, 27. The discovery of separate homologous regions on the virulence plasmids of Salmonella and Yersinia suggests a distant evolutionary relationship.

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supporting

confidence: 76%

Homologous DNA sequences on the virulence plasmids of pathogenic Yersinia and Salmonella dublin Lane

Krause

Fierer

Guiney

1990

Molecular Microbiology

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“…pestis genes (yopB, yopD, yopO, pla and yscF) were first amplified with pfu polymerase (Strategene, CA) from plasmids pCD1 or pPCP1. The yopB, yopD and yopO/ypkA are encoded by the 70 kb plasmid, pCD1 [13] and the pla is encoded by the 9.5 kb plasmid, pPCP1 [34][35]. Primer pairs are shown in Table 1.…”

Section: Construction Of Plague Dna Vaccinesmentioning

confidence: 99%

Relative immunogenicity and protection potential of candidate Yersinia Pestis antigens against lethal mucosal plague challenge in Balb/C mice

Wang

Joshi

Mboudjeka

et al. 2008

Vaccine

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Yersinia Pestis Outer proteins, plasminogen activator protease and Yop secretion protein F are necessary for the full virulence of Yesinia pestis and have been proposed as potential protective antigens for vaccines against plague. In the current study, we used DNA immunization as a tool to study the relative protective immunity of these proteins with a standardized intranasal challenge system in mice. While the natural full length gene sequences for most of these Y. pestis proteins did not display a good level of protein expression in vitro when delivered by a DNA vaccine vector, the overall immunogenicity of these wild type gene DNA vaccines was low in eliciting antigen-specific antibody responses and gene sequence modifications improved both of these parameters. However, even modified YopD, YopO and YscF antigens were only able to partially protect immunized mice at various levels against lethal challenge with Y. pestis KIM 1001 strain while no protection was observed with either the YopB or Pla antigens. These results demonstrate that DNA immunization is effective in screening, optimizing and comparing optimal antigen designs and immunogenicity of candidate antigens for the development of a subunit-based plague vaccine.

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“…The position of ISIO0 on the 9.5-kb plasmid was determined when restriction fragments of this plasmid [11] were hybridized with different Y. pestis and Y. pseudotuberculosis strains ( Fig. 1).…”

Section: Localization Of Lslo0 On 95-kb Plasmidmentioning

confidence: 99%

Nucleotide sequence and structural organization of Yersinia pestis insertion sequence IS100

Подладчикова

1994

FEMS Microbiology Letters

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Insertion sequence IS100 was localized on a 9.5-kb plasmid of Yersinia pestis and was shown to be specific for Y. pestis and serotype I strains of Y. pseudotuberculosis. The nucleotide sequence of IS100 isolated from this plasmid was determined. The element, which was flanked by 5-bp direct repeats, contained 1953 bp including imperfect inverted terminal repeats of 52 and 61 bp long (43 bp were identical). Two open reading frames encoding potential polypeptides of 340 and 252 amino acids were identified on one DNA strand. Nucleotide sequence as well as deduced polypeptide sequences of IS100 were homologous to those of IS21, IS232 and IS640.

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Genetic analysis of the 9.5-kilobase virulence plasmid of Yersinia pestis

Cited by 140 publications

References 30 publications

Homologous DNA sequences on the virulence plasmids of pathogenic Yersinia and Salmonella dublin Lane

Homologous DNA sequences on the virulence plasmids of pathogenic Yersinia and Salmonella dublin Lane

Relative immunogenicity and protection potential of candidate Yersinia Pestis antigens against lethal mucosal plague challenge in Balb/C mice

Nucleotide sequence and structural organization of Yersinia pestis insertion sequence IS100

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