Genetic analysis and gene mapping of a dwarf and liguleless mutation in barley

Guo, Bin; Jing, Qi; Li, Dongfang; Sun, Hongwei; Lyu, Chao; Wang, Feifei; Jun, Zhu; Guo, Ganggang; Xu, Ran

doi:10.1016/j.cj.2022.01.006

Cited by 3 publications

(1 citation statement)

References 41 publications

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“…Genomic DNA of parents and each F 2 plants were extracted by CTAB method (Clarke, 2009). InDel markers on the barley genome showing polymorphism between bc3 and Morex were designed for rough gene mapping (Guo et al, 2022) (Table S1). In the primary mapping, two DNA pools composed of 30 mutant and wild-type phenotype individuals and two parents DNA were used for the bulked segregation analysis (Michelmore et al, 1991).…”

Section: Map-based Cloning and Sequencingmentioning

confidence: 99%

Brittle culm 3, encoding a cellulose synthase subunit 5, is required for cell wall biosynthesis in barley (Hordeum vulgare L.)

et al. 2022

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The cell wall plays an important role in plant mechanical strength. Cellulose is the major component of plant cell walls and provides the most abundant renewable biomass resource for biofuels on earth. Mutational analysis showed that cellulose synthase (CESA) genes are critical in cell wall biosynthesis in cereal crops like rice. However, their role has not been fully elucidated in barley. In this study, we isolated a brittle culm mutant brittle culm 3 (bc3) derived from Yangnongpi 5 ethyl methanesulfonate (EMS) mutagenesis in barley. The bc3 mutants exhibited reduced mechanical strength of the culms due to impaired thickening of the sclerenchyma cell wall and reduced cellulose and hemicellulose content in the culms. Genetic analysis and map-based cloning revealed that the bc3 mutant was controlled by a single recessive gene and harbored a point mutation in the HvCESA5 gene, generating a premature stop codon near the N-terminal of the protein. Quantitative real-time PCR (qRT-PCR) analysis showed that the HvCESA5 gene is predominantly expressed in the culms and co-expressed with HvCESA4 and HvCESA8, consistent with the brittle culm phenotype of the bc3 mutant. These results indicate that the truncated HvCESA5 affects cell wall biosynthesis leading to a brittle culm phenotype. Our findings provide evidence for the important role of HvCESA5 in cell wall biosynthesis pathway and could be a potential target to modify cell wall in barley.

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Section: Map-based Cloning and Sequencingmentioning

confidence: 99%

Brittle culm 3, encoding a cellulose synthase subunit 5, is required for cell wall biosynthesis in barley (Hordeum vulgare L.)

et al. 2022

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A single nucleotide substitution in the MATE transporter gene regulates plastochron and the many noded dwarf phenotype in barley (Hordeum vulgare L.)

Guo

Sun

Jing

et al. 2023

Journal of Integrative Agriculture

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Advancements in genetic techniques and functional genomics for enhancing crop traits and agricultural sustainability

Kumar,

Singh,

Bist

et al. 2024

Briefings in Functional Genomics

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Genetic variability is essential for the development of new crop varieties with economically beneficial traits. The traits can be inherited from wild relatives or induced through mutagenesis. Novel genetic elements can then be identified and new gene functions can be predicted. In this study, forward and reverse genetics approaches were described, in addition to their applications in modern crop improvement programs and functional genomics. By using heritable phenotypes and linked genetic markers, forward genetics searches for genes by using traditional genetic mapping and allele frequency estimation. Despite recent advances in sequencing technology, omics and computation, genetic redundancy remains a major challenge in forward genetics. By analyzing close-related genes, we will be able to dissect their functional redundancy and predict possible traits and gene activity patterns. In addition to these predictions, sophisticated reverse gene editing tools can be used to verify them, including TILLING, targeted insertional mutagenesis, gene silencing, gene targeting and genome editing. By using gene knock-down, knock-up and knock-out strategies, these tools are able to detect genetic changes in cells. In addition, epigenome analysis and editing enable the development of novel traits in existing crop cultivars without affecting their genetic makeup by increasing epiallelic variants. Our understanding of gene functions and molecular dynamics of various biological phenomena has been revised by all of these findings. The study also identifies novel genetic targets in crop species to improve yields and stress tolerances through conventional and non-conventional methods. In this article, genetic techniques and functional genomics are specifically discussed and assessed for their potential in crop improvement.

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Genetic analysis and gene mapping of a dwarf and liguleless mutation in barley

Cited by 3 publications

References 41 publications

Brittle culm 3, encoding a cellulose synthase subunit 5, is required for cell wall biosynthesis in barley (Hordeum vulgare L.)

Brittle culm 3, encoding a cellulose synthase subunit 5, is required for cell wall biosynthesis in barley (Hordeum vulgare L.)

A single nucleotide substitution in the MATE transporter gene regulates plastochron and the many noded dwarf phenotype in barley (Hordeum vulgare L.)

Advancements in genetic techniques and functional genomics for enhancing crop traits and agricultural sustainability

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