Brittle culm 3, encoding a cellulose synthase subunit 5, is required for cell wall biosynthesis in barley (Hordeum vulgare L.)

Guo, Bin; Huang, Xinyu; Jing, Qi; Sun, Hongwei; Lv, Chao; Wang, Feifei; Jun, Zhu; Xu, Ran

doi:10.3389/fpls.2022.989406

Cited by 9 publications

(4 citation statements)

References 58 publications

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“…The circulation of CSCs between the plasma membrane and various intracellular compartments plays a crucial role in determining the level of cellulose synthesis ( Vellosillo et al, 2021 ). Studies have demonstrated that several factors, such as transcription factors like MYB family proteins, SND1, VND family proteins, and signaling molecules including NO, NAA, and BR, can promote cellulose synthesis ( Guo et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Research overview on the genetic mechanism underlying the biosynthesis of polysaccharide in tuber plants

Xu,

Hu,

et al. 2024

PeerJ

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Tuber plants are of great significance in the world as human food crops. Polysaccharides, important metabolites in tuber plants, also serve as a source of innovative drugs with significant pharmacological effects. These drugs are particularly known for their immunomodulation and antitumor properties. To fully exploit the potential value of tuber plant polysaccharides and establish a synthetic system for their targeted synthesis, it is crucial to dissect their metabolic processes and genetic regulatory mechanisms. In this article, we provide a comprehensive summary of the basic pathways involved in the synthesis of various types of tuber plant polysaccharides. We also outline the key research progress that has been made in this area in recent years. We classify the main types and functions of tuber plant polysaccharides and analyze the biosynthetic processes and genetic regulation mechanisms of key enzymes involved in the metabolic pathways of starch, cellulose, pectin, and fructan in tuber plants. We have identified hexokinase and glycosyltransferase as the key enzymes involved in the polysaccharide synthesis process. By elucidating the synthesis pathway of polysaccharides in tuber plants and understanding the underlying mechanism of action of key enzymes in the metabolic pathway, we can provide a theoretical framework for enhancing the yield of polysaccharides and other metabolites in plant culture cells. This will ultimately lead to increased production efficiency.

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Section: Introductionmentioning

confidence: 99%

Research overview on the genetic mechanism underlying the biosynthesis of polysaccharide in tuber plants

Xu,

Hu,

et al. 2024

PeerJ

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“…Brittle mutants have been extensively studied in various plant species, including rice [1,2], Arabidopsis thaliana [3,4], and barley [5]. The culms of these mutants typically exhibit decreased mechanical strength and increased brittleness.…”

Section: Introductionmentioning

confidence: 99%

Identification and Gene Cloning of a Brittle Culm Mutant (bc22) in Rice

Cao,

Zhou,

Sun

et al. 2024

Agriculture

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The mechanical strength of rice culm, an essential factor for lodging resistance and yield maintenance, is influenced by the composition and structure of the cell wall. In this study, we characterized a rice brittle culm mutant 22 (bc22), derived from LR005 through ethyl methanesulfonate (EMS) mutagenesis. The bc22 culm exhibited increased fragility and reduced mechanical strength compared to LR005. The mutant displayed pleiotropic effects, including a shorter plant height and panicle length, a smaller grain size, and the absence of the glume hairs. Scanning electron microscopy revealed a decrease in cell density and a looser structure in the bc22 culms. Biochemical analysis demonstrated a significant increase in hemicellulose content and a marked reduction in lignin content in the culm of bc22. Genetic analysis indicated that the brittle culm trait was governed by a single recessive gene. After employing bulked segregant analysis (BSA), whole-genome resequencing, and MutMap methods, LOC_Os02g25230 was identified as the candidate gene responsible for bc22. In bc22, a point mutation from proline (Pro) to leucine (Leu) in its coding region led to the pleiotropic phenotype. A complementation test further confirmed that the missense mutation causing the proline to leucine amino acid substitution in LOC_Os02g25230 was causative of the observed bc22 phenotype. Additionally, gene expression analysis showed that BC22 had higher expression levels in the culms, leaves, and spikelets compared to the roots. Taken together, our findings indicate that BC22 is a pleiotropic gene, and the influence of BC22 on brittleness may be associated with cell wall biosynthesis in rice culm.

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“…In barley (H. vulgare L.), the influence of cellulose synthase and cellulose synthase-like enzymes on the roots, stems, and associated biological reaction processes, achieved through their regulatory role in cellulose content, has been elucidated [15][16][17]. However, although one COBL gene associated with cellulose synthesis was pinpointed through genomewide association studies (GWAS) conducted to investigate cellulose content in barley [18], the information on COBL family genes and their effect on cellulose in barley remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification and Hormone Response Analysis of the COBL Gene Family in Barley

Ren,

Ma,

Bao

et al. 2024

Genes

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Barley (Hordeum vulgare L.), a diverse cereal crop, exhibits remarkable versatility in its applications, ranging from food and fodder to industrial uses. The content of cellulose in barley is significantly influenced by the COBRA genes, which encode the plant glycosylphosphatidylinositol (GPI)-anchored protein (GAP) that plays a pivotal role in the deposition of cellulose within the cell wall. The COBL (COBRA-Like) gene family has been discovered across numerous species, yet the specific members of this family in barley remain undetermined. In this study, we discovered 13 COBL genes within the barley genome using bioinformatics methods, subcellular localization, and protein structure analysis, finding that most of the barley COBL proteins have a signal peptide structure and are localized on the plasma membrane. Simultaneously, we constructed a phylogenetic tree and undertook a comprehensive analysis of the evolutionary relationships. Other characteristics of HvCOBL family members, including intraspecific collinearity, gene structure, conserved motifs, and cis-acting elements, were thoroughly characterized in detail. The assessment of HvCOBL gene expression in barley under various hormone treatments was conducted through qRT-PCR analysis, revealing jasmonic acid (JA) as the predominant hormonal regulator of HvCOBL gene expression. In summary, this study comprehensively identified and analyzed the barley COBL gene family, aiming to provide basic information for exploring the members of the HvCOBL gene family and to propose directions for further research.

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Brittle culm 3, encoding a cellulose synthase subunit 5, is required for cell wall biosynthesis in barley (Hordeum vulgare L.)

Cited by 9 publications

References 58 publications

Research overview on the genetic mechanism underlying the biosynthesis of polysaccharide in tuber plants

Research overview on the genetic mechanism underlying the biosynthesis of polysaccharide in tuber plants

Identification and Gene Cloning of a Brittle Culm Mutant (bc22) in Rice

Genome-Wide Identification and Hormone Response Analysis of the COBL Gene Family in Barley

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