Generation of anti-zearalenone scFv and its incorporation into surface plasmon resonance-based assay for the detection of zearalenone in sorghum

Edupuganti, Soujanya Ratna; Edupuganti, Om Prakash; O’Kennedy, Richard

doi:10.1016/j.foodcont.2013.06.013

Cited by 27 publications

(15 citation statements)

References 23 publications

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“…The key limitations of these methods include application of bulky and expensive instruments, which are difficult to use in-field, have a long measurement time, and have quite complicated detection protocols requiring highly equipped laboratories and well-trained personnel. Therefore, immunochemical test systems with electrochemical detection of the label [19], magnetic and amperometric biosensors [20,21], as well as SPR-based immunosensors [22] were developed for real-time analysis of zearalenone. The present research is aimed at the development of an optical sensor system based on synthetic receptors capable of selective recognition of zearalenone toxin in the complex food matrix.…”

Section: Introductionmentioning

confidence: 99%

Sensor Based on Molecularly Imprinted Polymer Membranes and Smartphone for Detection of Fusarium Contamination in Cereals

Sergeyeva

Yarynka

Dubey

et al. 2020

Sensors

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The combination of the generic mobile technology and inherent stability, versatility and cost-effectiveness of the synthetic receptors allows producing optical sensors for potentially any analyte of interest, and, therefore, to qualify as a platform technology for a fast routine analysis of a large number of contaminated samples. To support this statement, we present here a novel miniature sensor based on a combination of molecularly imprinted polymer (MIP) membranes and a smartphone, which could be used for the point-of-care detection of an important food contaminant, oestrogen-like toxin zearalenone associated with Fusarium contamination of cereals. The detection is based on registration of natural fluorescence of zearalenone using a digital smartphone camera after it binds to the sensor recognition element. The recorded image is further processed using a mobile application. It shows here a first example of the zearalenone-specific MIP membranes synthesised in situ using “dummy template”-based approach with cyclododecyl 2, 4-dihydroxybenzoate as the template and 1-allylpiperazine as a functional monomer. The novel smartphone sensor system based on optimized MIP membranes provides zearalenone detection in cereal samples within the range of 1–10 µg mL−1 demonstrating a detection limit of 1 µg mL−1 in a direct sensing mode. In order to reach the level of sensitivity required for practical application, a competitive sensing mode is also developed. It is based on application of a highly-fluorescent structural analogue of zearalenone (2-[(pyrene-l-carbonyl) amino]ethyl 2,4-dihydroxybenzoate) which is capable to compete with the target mycotoxin for the binding to zearalenone-selective sites in the membrane’s structure. The competitive mode increases 100 times the sensor’s sensitivity and allows detecting zearalenone at 10 ng mL−1. The linear dynamic range in this case comprised 10–100 ng mL−1. The sensor system is tested and found effective for zearalenone detection in maize, wheat and rye flour samples both spiked and naturally contaminated. The developed MIP membrane-based smartphone sensor system is an example of a novel, inexpensive tool for food quality analysis, which is portable and can be used for the “field” measurements and easily translated into the practice.

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Section: Introductionmentioning

confidence: 99%

Sensor Based on Molecularly Imprinted Polymer Membranes and Smartphone for Detection of Fusarium Contamination in Cereals

Sergeyeva

Yarynka

Dubey

et al. 2020

Sensors

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“…Transcriptome-wide mapping of N 6 -methyladenosine (m 6 A), an abundant modification on eukaryotic mRNA, [4][5] has led to the proposal of RNA epigenetics or epitranscriptomics [6][7] . N 6 -methyladenosine perturbs RNA structure 8 and functions as an epitope for the recruitment of modification-specific reader proteins [9][10] , resulting in the modulation of RNA stability 11 , protein translation 12 , splicing 13 , and nuclear export 14 . Recent studies have demonstrated a role for the m 6 A pathway in diverse biology occurring in normal [15][16][17] and disease [18][19] contexts.…”

Section: Introductionmentioning

confidence: 99%

YTHDF2 Recognition of N¹-Methyladenosine (m¹A)-Modified RNA Is Associated with Transcript Destabilization

Seo

Kleiner

2019

ACS Chem. Biol.

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Epitranscriptomic modifications play an important role in RNA function and can impact gene expression. Here, we apply a chemical proteomics approach to investigate readers of N 1methyladenosine (m 1 A), a poorly characterized modification on mammalian mRNA. We find that YTHDF proteins, known m 6 A readers, recognize m 1 A-modified sequences in a methylationspecific manner. We characterize binding of recombinant YTHDF1/2 proteins to m 1 A-modified oligonucleotides to demonstrate that these interactions can exhibit comparable affinity to m 6 Arecognition events, and occur in diverse sequence contexts. Further, we demonstrate YTHDF2 interacts specifically with endogenously modified m 1 A transcripts. Finally, we deplete cellular YTHDF2 to show that the abundance of m 1 A-modified transcripts is increased in its absence. Similarly, increasing m 1 A levels through depletion of ALKBH3, an m 1 A eraser protein, destabilizes known m 1 A-containing RNAs. Our results shed light on the function of m 1 A on mRNA, and provide a mechanistic framework to further evaluate the role of m 1 A in biological processes.

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“…Since the early 2000's, commercially-available laboratory-based systems founded on the principle of surface plasmon resonance have been effectively used for the detection of mycotoxins in a variety of matrices including DON in wheat (Meneely et al 2010), AFM1 in milk (Wang et al 2009), OTA in cereals and beverages (Yuan et al 2009), T2/HT2 in cereals (Meneely et al 2012) and ZEN in sorghum (Edupuganti et al 2013). SPR is an optical phenomenon that occurs when polarised light hits a metal film between two media of different refractive index.…”

Section: Surface Plasmon Resonancementioning

confidence: 99%

Current trends in rapid tests for mycotoxins

Nolan

Auer²,

Spehar³

et al. 2019

Food Additives & Contaminants: Part A

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There is an ample number of commercial testing kits available for mycotoxin analysis on the market today, including enzyme-linked immunosorbent assays, membrane-based immunoassays, fluorescence polarisation immunoassays and fluorometric assays. It can be observed from the literature that not only are developments and improvements ongoing for these assays but there are also novel assays being developed using biosensor technology. This review focuses on both the currently available methods and recent innovative methods for mycotoxin testing. Furthermore, it highlights trends that are influencing assay developments such as multiplexing capabilities and rapid on-site analysis, indicating the possible detection methods that will shape the future market.

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Generation of anti-zearalenone scFv and its incorporation into surface plasmon resonance-based assay for the detection of zearalenone in sorghum

Cited by 27 publications

References 23 publications

Sensor Based on Molecularly Imprinted Polymer Membranes and Smartphone for Detection of Fusarium Contamination in Cereals

Sensor Based on Molecularly Imprinted Polymer Membranes and Smartphone for Detection of Fusarium Contamination in Cereals

YTHDF2 Recognition of N¹-Methyladenosine (m¹A)-Modified RNA Is Associated with Transcript Destabilization

Current trends in rapid tests for mycotoxins

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Generation of anti-zearalenone scFv and its incorporation into surface plasmon resonance-based assay for the detection of zearalenone in sorghum

Cited by 27 publications

References 23 publications

Sensor Based on Molecularly Imprinted Polymer Membranes and Smartphone for Detection of Fusarium Contamination in Cereals

Sensor Based on Molecularly Imprinted Polymer Membranes and Smartphone for Detection of Fusarium Contamination in Cereals

YTHDF2 Recognition of N1-Methyladenosine (m1A)-Modified RNA Is Associated with Transcript Destabilization

Current trends in rapid tests for mycotoxins

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Product

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YTHDF2 Recognition of N¹-Methyladenosine (m¹A)-Modified RNA Is Associated with Transcript Destabilization