Generation and characterization of novel DNA aptamers against coat protein of grouper nervous necrosis virus (GNNV) with antiviral activities and delivery potential in grouper cells

Molecular biology of Macrobrachium rosenbergii nodavirus infection in giant freshwater prawn

“…With the advancement in different molecular biotechnologies, a heap of molecular‐based prophylactic and/or therapeutic agents has been reported. These include the technology of RNA interference (Itsathitphaisarn, Thitamadee, Weerachatyanukul, & Sritunyalucksana, ; Jitrakorn, Chucherd, & Saksmerprome, ; Reshi, Wu, Wang, & Hong, ), recombinant DNA technology (Kumar, Karunasagar, & Karunasagar, ; Sahoo et al., ) and technology of aptamers that is also known as the synthetic antibody (Aoki & Hirono, ; Hwang et al., ; Li et al., ; Li, Zhou, et al., ; Zhou et al., ). Scanty reports of other approaches, for instances the assessment of genetic variation/inheritance in selective breeding of disease‐resistant strains (Luan et al., ) and the use of natural products of plant extracts (Farook et al., ; Khanna, Kannabiran, Babu, & Hameed, ), are available.…”

“…To date, the application of aptamers technology in infectious disease diagnosis of aquatic animal is mostly limited to viral infections. Several aptamers have been reported for viral detections (Aoki & Hirono, ; Li et al., ; Li, Zhou, et al., , Zhou et al., , ) and the detection of virus‐infected cells (Li, Wei, et al., ; Li et al., , ), and aptamers that manifesting antiviral property (Hwang et al., ; Li et al., ; Li, Zhou, et al., ; Zhou et al., ).…”

First identification of the nervous necrosis virus isolated from cultured golden pompano (Trachinotus ovatus) in Guangxi, China

“…As NNV is one of the most devastating pathogens in the marine aquaculture, and no effective methods for controlling NNV infections are available at present, then early rapid detection of NNV infections would be greatly helpful for timely treatment, including removing the infected fish and water purification. The novel aptamer‐based sandwich enzyme‐linked apta‐sorbent assay (ELASA) for RGNNV diagnosis has been developed in the previous study (Zhou et al., , 2017b). The brain tissues of suspected NNV‐infected golden pompano were cut into pieces in PBS and lysed in 200 μl Pierce™ IP Lysis Buffer.…”

Development of novel aptamer‐based enzyme‐linked apta‐sorbent assay (ELASA) for rapid detection of mariculture pathogen Vibrio alginolyticus

“…At present, aptamers have made great progress in the ichthyopathology field. The aptamers against some severe aquatic viruses, such as Singapore grouper iridovirus (Li et al, ; Li, Wei, et al, ; Li, Zhou, et al, ), red‐spotted grouper nervous necrosis virus (Zhou et al, ), Trachinotus ovatus NNV (Yu, Liu, Wei, et al, ), spring viraemia of carp virus (Yu et al, ), Hirame rhabdovirus virus (Hwang et al, ), viral haemorrhagic septicaemia virus (Punnarak et al, ) and soft‐shelled turtle iridovirus (Li, Wei, et al, ; Li, Zhou, et al, ), have been successfully generated. There are also kinds of aptamers generated against various bacterial targets, such as Lactobacillus acidophilus , V. parahaemolyticus and Salmonella typhimurium (Duan, Wu, Chen, Huang, & Wang, ; Duan et al, ), V. alginolyticus (Yu, Liu, Su, et al, ; Tang, Zheng, Yan, Li, & Li, ; Zheng et al, ), V. harveyi (Zheng, Hao, Song, & Liu, ) and Aeromonas hydrophila (Li, Wang, Chen, & Li, ).…”

“…There are also kinds of aptamers generated against various bacterial targets, such as Lactobacillus acidophilus , V. parahaemolyticus and Salmonella typhimurium (Duan, Wu, Chen, Huang, & Wang, ; Duan et al, ), V. alginolyticus (Yu, Liu, Su, et al, ; Tang, Zheng, Yan, Li, & Li, ; Zheng et al, ), V. harveyi (Zheng, Hao, Song, & Liu, ) and Aeromonas hydrophila (Li, Wang, Chen, & Li, ). Aptamers have been widely applied in different important research areas, including drug‐specific delivery (Loo et al, ; Zhao et al, ), pathogen diagnosis (Li et al, ; Li, Wei, et al, ; Li, Zhou, et al, ; Teng et al, ; Yu, Liu, Wei, et al, ; Zhou et al, ), toxin analysis (Huang et al, ; Yan et al, ) and disease pathogenesis research (Bunka & Stockley, ). The excellent characteristics of easy synthesis, low immunogenicity, innocuity and high specificity and stability make aptamers ideal alternatives to antibodies (Toh et al, ).…”