First identification of the nervous necrosis virus isolated from cultured golden pompano (<i>Trachinotus ovatus</i>) in Guangxi, China

Li, P; Yu, Qing; Li, F; Qin, Xianling; Dong, Dexin; Chen, B; Qin, Qiwei

doi:10.1111/jfd.12805

Cited by 12 publications

(7 citation statements)

References 16 publications

(20 reference statements)

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“…Furthermore, the presence of viral particles of LMNNV‐GD was confirmed by transmission electron microscopy. Electron microscopy revealed the aggregation of numerous non‐envelope viral particles with a diameter of about 20–30 nm in the cytoplasm of the virus‐infected cells (Figure 3b), which is consistent with previous findings of NNV (Li, Yu, et al., 2018).…”

Section: Results and Disscussionsupporting

confidence: 91%

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Identification and characterization of a nervous necrosis virus isolated from largemouth bass (Micropterus salmoides)

Cai

Xia

et al. 2022

Journal of Fish Diseases

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Section: Results and Disscussionsupporting

confidence: 91%

“…Largemouth bass ( Micropterus salmoides ) is an important aquaculture species, cultured worldwide and in China (Chen et al., 2013; Li et al., 2018a; Li, Yu, et al., 2018). In recent year, outbreaks of viral diseases have affected largemouth bass farming in China.…”

Section: Inroductionmentioning

confidence: 99%

Identification and characterization of a nervous necrosis virus isolated from largemouth bass (Micropterus salmoides)

Cai

Xia

et al. 2022

Journal of Fish Diseases

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“…Since the first descriptions in the 1990s, VER episodes have been constantly reported, mainly in marine fish reared in Asian, Australian and European waters [57,74,86,90,91,115,117,120,245]. The most important affected species include grouper, Asian seabass/barramundi, European sea bass, gilthead sea bream, Japanese and barfin flounder, Atlantic and Pacific cod and Atlantic halibut (Table 1).…”

Section: Ver Outbreaks and Nnv Detections In Farmed Fishmentioning

confidence: 99%

Betanodavirus and VER Disease: A 30-year Research Review

2020

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The outbreaks of viral encephalopathy and retinopathy (VER), caused by nervous necrosis virus (NNV), represent one of the main infectious threats for marine aquaculture worldwide. Since the first description of the disease at the end of the 1980s, a considerable amount of research has gone into understanding the mechanisms involved in fish infection, developing reliable diagnostic methods, and control measures, and several comprehensive reviews have been published to date. This review focuses on host-virus interaction and epidemiological aspects, comprising viral distribution and transmission as well as the continuously increasing host range (177 susceptible marine species and epizootic outbreaks reported in 62 of them), with special emphasis on genotypes and the effect of global warming on NNV infection, but also including the latest findings in the NNV life cycle and virulence as well as diagnostic methods and VER disease control.

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“…and artificial breeding technologies have gradually transformed grouper farming in China into a large-scale aquaculture industry. However, with the expansion of caged aquaculture, the various diseases affecting grouper populations in southern China have become increasingly serious [2][3][4]. Singapore grouper iridovirus (SGIV) is a recently discovered serious infectious virus of fish and is currently the main pathogen causing severe economic losses in grouper aquaculture.…”

Section: Introductionmentioning

confidence: 99%

Antiviral Activities of Green Tea Components against Grouper Iridovirus Infection In Vitro and In Vivo

Huang

Xiao

et al. 2022

Viruses

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(1) Background: Singapore grouper iridovirus (SGIV) can cause extensive fish deaths. Therefore, developing treatments to combat virulent SGIV is of great economic importance to address this challenge to the grouper aquaculture industry. Green tea is an important medicinal and edible plant throughout the world. In this study, we evaluated the use of green tea components against SGIV infection. (2) Methods: The safe working concentrations of green tea components were identified by cell viability detection and light microscopy. Additionally, the antiviral activity of each green tea component against SGIV infection was determined with light microscopy, an aptamer (Q5c)-based fluorescent molecular probe, and reverse transcription quantitative PCR. (3) Results: The safe working concentrations of green tea components were green tea aqueous extract (GTAE) ≤ 100 μg/mL, green tea polyphenols (TP) ≤ 10 μg/mL, epigallocatechin-3-gallate (EGCG) ≤ 12 μg/mL, (-)-epigallocatechin (EGC) ≤ 10 μg/mL, (-)-epicatechin gallate (EGC) ≤ 5 μg/mL, and (-)-epicatechin (EC) ≤ 50 μg/mL. The relative antiviral activities of the green tea components determined in terms of MCP gene expression were TP > EGCG > GTAE > ECG > EGC > EC, with inhibition rates of 99.34%, 98.31%, 98.23%, 88.62%, 73.80%, and 44.31%, respectively. The antiviral effect of aptamer-Q5c was consistent with the results of qPCR. Also, TP had an excellent antiviral effect in vitro, wherein the mortality of fish in only the SGIV-injection group and TP + SGIV-injection group were 100% and 11.67%, respectively. (4) Conclusions: In conclusion, our results suggest that green tea components have effective antiviral properties against SGIV and may be candidate agents for the effective treatment and control of SGIV infections in grouper aquaculture.

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First identification of the nervous necrosis virus isolated from cultured golden pompano (Trachinotus ovatus) in Guangxi, China

Cited by 12 publications

References 16 publications

Identification and characterization of a nervous necrosis virus isolated from largemouth bass (Micropterus salmoides)

Identification and characterization of a nervous necrosis virus isolated from largemouth bass (Micropterus salmoides)

Betanodavirus and VER Disease: A 30-year Research Review

Antiviral Activities of Green Tea Components against Grouper Iridovirus Infection In Vitro and In Vivo

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