Gene transcript changes in individual rainbow trout livers following an inflammatory stimulus

Acute phase gene expression in rainbow trout (Oncorhynchus mykiss) after exposure to a confinement stressor: A comparison of pooled and individual data

“…Pentraxin gene expression was found to be significantly decreased (approximately 2 fold) at the later times of 48 h and 168 h suggesting a possible role for SAP-like pentraxins as negative acute phase proteins during periods of prolonged confinement. Pentraxin, both at the level of protein and mRNA expression has been reported as a negative acute phase protein in trout after activation of the acute phase response by bacterial challenge (Jensen et al, 1997;Gerwick et al, 2007).…”

“…Induction of the acute phase response in fish is most frequently associated with the activation of the inflammatory response, typically in response to a pathogen challenge Gerwick et al, 2007;Martin et al, 2006). The acute phase response proteins (APPs) are regarded as having general functions in opsonisation and trapping of micro-organisms and their products, in activating complement, in binding cellular remnants, in neutralising enzymes, scavenging free haemoglobin and radicals and in modulating the immune response (Gurys et al, 2005).…”

“…These techniques have been used in many recent studies to investigate the acute phase response in fish after bacterial challenge in rainbow trout (Oncorhynchus mykiss) Gerwick et al, 2007) and in Atlantic salmon (Salmo salar) (Tsoi et al, 2004;Ewart et al, 2005;Martin et al, 2006). A panel of eight candidate genes was chosen for evaluation of acute phase response induction.…”

“…Two other less well characterised genes selected for analysis were LECT2 and DRTP1. Expression of the LECT2 gene has been reported to increase markedly during the acute phase response in zebrafish (Danio rerio) (Lin et al, 2007) while the DRTP1 gene has been shown to be differentially regulated during the acute phase response in salmon (Martin et al, 2006) and trout Gerwick et al, 2007). The induction of DRTP1, in common with other acute phase proteins, has NF-κΒ and C/EBPβ as important transcription factors involved in its induction (Talbot et al, 2008).…”

Characterisation of the differentially regulated trout protein 1 (DRTP1) gene in rainbow trout (Oncorhynchus mykiss)

“…DRTP1 was identified as the most abundant clone in the resulting liver SSH library [2]. Through the use of an oligo nucleotide cDNA microarray combined with quantitative real-time PCR (qPCR) these authors further validated the increase in DRTP1 gene expression [3].…”