Characterisation of the differentially regulated trout protein 1 (DRTP1) gene in rainbow trout (Oncorhynchus mykiss)

Talbot, Anita; Smith, Terry J.; Cairns, Michael T.

doi:10.1016/j.fsi.2008.09.013

Cited by 8 publications

(4 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Functional feed additives are thought to modulate the local immune response in the intestine and several of the investigated genes in the anterior intestine were modulated by the diet. The increased expression of drtp1 in all microalgae supplemented groups may be linked to a general response of the intestine towards novel antigens in the diet, but was also found to be induced after an acute phase response (Martin et al, 2006;Talbot et al, 2009). Interferon-stimulated gene 15 (isg15) was upregulated in the anterior intestine in response to the microalgae diets.…”

Section: And Including Chlorellamentioning

confidence: 97%

Microalgae as functional feed for Atlantic salmon: effects on growth, health, immunity, muscle fatty acid and pigment deposition

Mueller,

Pauly,

Molkentin

et al. 2023

Front. Mar. Sci.

View full text Add to dashboard Cite

Microalgae are increasingly being investigated as functional feed additives in a variety of fish species, but our knowledge on how microalgae supplementation affects Atlantic salmon remains limited. We hypothesized that microalgae inclusion of 8% in the feed would improve performance, fatty acid and pigment deposition as well as health and immunity of Atlantic salmon reared in recirculating aquaculture systems (RAS). We fed Atlantic salmon smolts with five different microalgae enriched diets containing Tetraselmis chuii (TC), Arthrospira platensis (AP), Schizochytrium limacinum (SL) or Chlorella vulgaris, either intact (CVI) or as broken cell wall derivative (CVB) or a control diet (CD). After eight weeks of feeding in brackish water (13 psu), all groups were transferred to seawater (32 psu) for additional two weeks. Our results indicate that CVB improved feed conversion and protein retention, but reduced condition factor (p < 0.05) compared to fish fed with a control diet. Voluntary feed intake decreased in seawater, but was similar among diet groups. The amount of docosahexaenoic acid was particularly high in SL-fed fish and alpha-linolenic acid was enriched in fish fed CVI, CVB and TC (p < 0.05). Following seawater transfer, fat content and monounsaturated fatty acids decreased in the muscle, while polyunsaturated fatty acids increased. Lutein was present in all muscle samples, but highest concentrations were found in CVB-, CVI- and TC-fed fish. In the anterior intestine, microalgae supplementation induced differentially regulated trout protein 1 (drtp1) expression in CVI- and CVB-fed fish, but reduced the expression of interleukin 1 and 10 receptor (il1r2 & il10rb) in CVI-fed fish. In the liver, feeding CVI and SL induced complement C1q like 2 (c1ql2) expression, while reducing serum amyloid A5 (saa5) expression. Superoxide-dismutase protein concentration was induced in the liver of fish fed SL, while myeloperoxidase was reduced in most microalgae-fed groups. In conclusion, we show that commercially relevant microalgae can be used as functional feed additives for Atlantic salmon promoting different health aspects without negatively affecting their growth performance when cultivated in RAS.

show abstract

Section: And Including Chlorellamentioning

confidence: 97%

Microalgae as functional feed for Atlantic salmon: effects on growth, health, immunity, muscle fatty acid and pigment deposition

Mueller,

Pauly,

Molkentin

et al. 2023

Front. Mar. Sci.

View full text Add to dashboard Cite

show abstract

“…Drtp1 belongs to APR and may participate in phospholipase inhibition, pathogen response, and the stress reaction [86]. Previously, it was shown that drtp1 gene expression level increased in the liver of ayu (Plecoglossus altivelis) during Listonella anguillarum infection [87].…”

Section: Expression Of Acute Phase Response Genes Saa and Drtp1mentioning

confidence: 99%

Proinflammatory cytokine and cytokine receptor gene expression kinetics following challenge with Flavobacterium psychrophilum in resistant and susceptible lines of rainbow trout (Oncorhynchus mykiss)

Kutyrev

Cleveland

Leeds

et al. 2016

Fish & Shellfish Immunology

View full text Add to dashboard Cite

Flavobacterium psychrophilum (Fp) is the causative agent of bacterial cold water disease (BCWD) which causes appreciable economic losses in rainbow trout aquaculture. We previously reported development of a genetic line, designated ARS-Fp-R that exhibits higher survival relative to a susceptible line, designated ARS-Fp-S, following either laboratory or natural on-farm challenge. The objectives of this study were to determine the temporal kinetics of gene expression between experimentally-challenged ARS-Fp-R and ARS-Fp-S fish and the correlation between gene expression and pathogen load. We developed a GeXP multiplex RT-PCR assay to simultaneously examine expression of immune-relevant genes, concentrating on tumor necrosis factor and interleukin-1 ligand/receptor systems and acute phase response genes. Spleen tissue was sampled at 6 h, 24 h, 48 h and 144 h post-challenge and pathogen load quantified by qPCR. Transcript abundance of cytokine genes tnfa1, tnfa2, tnfa3, il1b1, il1b2, il11a; acute phase response genes saa and drtp1; and putative cytokine receptors il1r1-like-b, il1r2, tnfrsf1a, tnfrsf9, tnfrsf1a-like-b increased following challenge while the transcript abundance of il1r-like-1 and tnfrsf1a-like-a decreased compared to PBS-injected line-matched control fish. Principal component analysis identified transcript levels of genes il1r-like-1 and tnfrsf1a-like-a as exhibiting differential expression between genetic lines. In summary, Fp i.p. injection challenge elicited a proinflammatory cytokine gene expression response in the spleen, with ARS-Fp-R line fish exhibiting modestly higher basal expression levels of several putative cytokine receptors. This study furthers the understanding of the immune response following Fp challenge and differences in gene expression associated with selective breeding for disease resistance.

show abstract

“…A NAT complementary to differentially regulated trout protein 1 was the most upregulated common NAT during the late response in ARS-Fp-S and ARS-Fp-R genetic lines. High levels of differentially regulated trout protein 1 transcript indicate that the acute phase response has been activated ( 98 ). LncNATs complementary to other immune-related genes, including complement factor H, lysozyme II, complement C4, and interferon-induced protein 44, were also shared between the two genetic lines during late response.…”

Section: Resultsmentioning

confidence: 99%

Genome-wide identification of antisense lncRNAs and their association with susceptibility to Flavobacterium psychrophilum in rainbow trout

Ali¹,

Salem²

2022

Front. Immunol.

View full text Add to dashboard Cite

Eukaryotic genomes encode long noncoding natural antisense transcripts (lncNATs) that have been increasingly recognized as regulatory members of gene expression. Recently, we identified a few antisense transcripts correlating in expression with immune-related genes. However, a systematic genome-wide analysis of lncNATs in rainbow trout is lacking. This study used 134 RNA-Seq datasets from five different projects to identify antisense transcripts. A total of 13,503 lncNATs were identified genome-wide. About 75% of lncNATs showed multiple exons compared to 36.5% of the intergenic lncRNAs. RNA-Seq datasets from resistant, control, and susceptible rainbow trout genetic lines with significant differences in survival rate following Flavobacterium psychrophilum (Fp) infection were analyzed to investigate the potential role of the lncNATs during infection. Twenty-four pairwise comparisons between the different genetic lines, infectious status, and time points revealed 581 differentially expressed (DE) lncNATs and 179 differentially used exons (DUEs). Most of the DE lncNATs strongly and positively correlated in expression with their corresponding sense transcripts across 24 RNA-Seq datasets. LncNATs complementary to genes related to immunity, muscle contraction, proteolysis, and iron/heme metabolism were DE following infection. LncNATs complementary to hemolysis-related genes were DE in the resistant fish compared to susceptible fish on day 5 post-infection, suggesting enhanced clearance of free hemoglobin (Hb) and heme and increased erythropoiesis. LncNATs complementary to hepcidin, a master negative regulator of the plasma iron concentration, were the most downregulated lncNATs on day 5 of bacterial infection in the resistant fish. Ninety-four DE lncNAT, including five complementary to hepcidin, are located within 26 QTL regions previously identified in association with bacterial cold water disease (BCWD) in rainbow trout. Collectively, lncNATs are involved in the molecular architecture of fish immunity and should be further investigated for potential applications in genomic selection and genetic manipulation in aquaculture.

show abstract

Characterisation of the differentially regulated trout protein 1 (DRTP1) gene in rainbow trout (Oncorhynchus mykiss)

Cited by 8 publications

References 35 publications

Microalgae as functional feed for Atlantic salmon: effects on growth, health, immunity, muscle fatty acid and pigment deposition

Microalgae as functional feed for Atlantic salmon: effects on growth, health, immunity, muscle fatty acid and pigment deposition

Proinflammatory cytokine and cytokine receptor gene expression kinetics following challenge with Flavobacterium psychrophilum in resistant and susceptible lines of rainbow trout (Oncorhynchus mykiss)

Genome-wide identification of antisense lncRNAs and their association with susceptibility to Flavobacterium psychrophilum in rainbow trout

Contact Info

Product

Resources

About