Gene expression response to cisplatin treatment in drug-sensitive and drug-resistant ovarian cancer cells

Connexin 43 Enhances the Cisplatin-Induced Cytotoxicity in Mesothelioma Cells

“…On the other hand CDDP exerted only a low effect on GJIC though the drug caused activation of MAP kinase protein (Procházka et al, 2007). Another report which supports Procházka et al showed that Cx43 level was elevated in CDDP-resistant cell line (Li et al, 2007). Li et al indicated the conflicting reports in which the role of Cxs in cell survival were different; in case GJ component protein, they have been implicated the bystander effect, but Cx43 protein itself has also been shown to be important in cell survival, although this function is poor characterized.…”

“…Li et al indicated the conflicting reports in which the role of Cxs in cell survival were different; in case GJ component protein, they have been implicated the bystander effect, but Cx43 protein itself has also been shown to be important in cell survival, although this function is poor characterized. They also suggested that although the selection of CDDP resistance might have favored cells that expressed high level of Cx43, continued expression of this gene might decrease drug resistance by promoting a bystander effect, and finally concluded that Cx43 played a role in drug sensitivity in any case (Li et al, 2007). Meanwhile other reports showed that compounds like CDDP suppressed intercellular communication (Jo et al, 2005;Wang et al, 2000).…”

Establishment and characterization of the human SaTM-1 anal canal squamous cell carcinoma cell line derived from lymph node metastasis

“…We additionally confirmed that SaTM-1 cells could not express endothelial markers, such as CD31, LYVE-1 using immunohistochemistry to omit the possibility of contamination of endothelial cells. Furthermore, SaTM-1 cells formed tumors in nude mice at subcutaneous injection of 1x10 6 cells for each subline (Table II). Heterotransplantation of SaTM-1 cells into immunodeficent mice will provide a useful model for the study of human anal canal SCC whose incidence was extremely low.…”

“…Subconfluent SaTM-1 cells were harvested with trypsin-EDTA and resuspended to a final concentration of 1x10 6 and 1x10 7 cells/ml in Hank's balanced salt solution (Invitrogen). Five female immunodeficient mice (5 weeks old, BALB/c-nu/nu) purchased from CLEA Japan were inoculated subcutaneously at the left and right flanks with 1x10 5 and 1x10 6 cells/ 0.1 ml using a 26-gauge needle attached to a 1 ml syringe, respectively.…”

The Gene Expression Profiles of Medulloblastoma Cell Lines Resistant to Preactivated Cyclophosphamide

“…In hepatocellular carcinoma cells, its overexpression decreased cell death mediated by SIAH1 (seven in absentia homolog 1 (Drosophila)) [39]. GJA1 (gap junction protein, alpha 1, 43kDa (connexin 43)), also with elevated RNA level in D341 MED (4-HCR), was upregulated in cisplatin-resistant ovarian cancer cells [40]. However, the authors found that inhibition of connexin 43 function in such cells actually led to increase in drug resistance [40].…”