Establishment and characterization of the human SaTM-1 anal canal squamous cell carcinoma cell line derived from lymph node metastasis

Takeda,

doi:10.3892/ijmm_00000254

Cited by 5 publications

(4 citation statements)

References 8 publications

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“…first anal SCC cell line was reported (SaTM-1), which was isolated from a lymph node metastasis of a patient undergoing an inguinal lymphadenectomy [92], but the HPV status of this cell line was not reported and to date there are no published studies using this cell line.…”

Section: Accumula On Of Muta Onsmentioning

confidence: 99%

The Human Papillomavirus as a Common Pathogen in Oropharyngeal, Anal and Cervical Cancers

et al. 2019

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The burden of human papillomavirus (HPV)-related cancers worldwide is significant. Although the incidence of cervical cancer is decreasing due to cervical screening programmes, the incidences of oropharyngeal, anal and vulval cancers are increasing. The introduction of HPV vaccination programmes in many countries has had an impact on HPV infection rates but due to the time-lag from initial HPV infection to the development of invasive carcinoma, the impact on the incidence of HPV-related cancer will take more time to become evident. This review explores the common aspects of HPV-related cancers and how they differ from their HPV-negative counterparts, both clinically and molecularly. It also covers the implications this has on future treatment strategies, including the possible role of immunotherapy.

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Section: Accumula On Of Muta Onsmentioning

confidence: 99%

The Human Papillomavirus as a Common Pathogen in Oropharyngeal, Anal and Cervical Cancers

et al. 2019

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“…Existing preclinical models of ASCC include a cell line derived from a lymph node metastasis, two transgenic mouse models and a xenograft from a single patient. [44][45][46][47] Here we present a patient derived xenograft model and provide preliminary evidence of a promising, new therapeutic target for the management of ASCC.…”

Section: Discussionmentioning

confidence: 99%

Doublecortin‐like kinase 1 is a therapeutic target in squamous cell carcinoma

Standing

Arnold

Dandawate

et al. 2022

Molecular Carcinogenesis

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“…In addition, validated preclinical models of ASCC that accurately replicate clinical observations are limited. Existing preclinical models of ASCC include a cell line derived from a lymph node metastasis, two transgenic mouse models and a xenograft from a single patient (44–47). Here we present a patient derived xenograft model and provide preliminary evidence of a promising, new therapeutic target for the management of ASCC.…”

Section: Discussionmentioning

confidence: 99%

Doublecortin-like kinase 1 is a therapeutic target in squamous cell carcinoma

Standing

Arnold

Dandawate

et al. 2022

Preprint

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Doublecortin like kinase 1 (DCLK1) plays a crucial role in several cancers including colon and pancreatic adenocarcinomas. However, its role in squamous cell carcinoma (SCC) remains unknown. To this end, we examined DCLK1 expression in head and neck squamous cell carcinoma (HNSCC) and anal squamous cell carcinoma (ASCC). We found that DCLK1 is elevated in patient SCC tissue, which correlated with cancer progression and poorer overall survival. Furthermore, DCLK1 expression is significantly elevated in HPV negative cancer tissues, which are typically aggressive with poor responses to radiation therapy. To understand the role of DCLK1 in tumorigenesis, we used specific shRNA to suppress DCLK1 expression. This significantly reduced tumor growth, spheroid formation, and migration of HNSCC cancer cells. To further the translational relevance of our studies, we sought to identify a selective DCLK1 inhibitor. Current attempts to target DCLK1 using pharmacologic approaches have relied on non-specific suppression of DCLK1 kinase activity. Here, we demonstrate that DiFiD [3,5-bis (2,4-difluorobenzylidene)-4-piperidone] binds to DCLK1 with high selectivity. Moreover, DiFiD mediated suppression of DCLK1 led to G2/M arrest and apoptosis and significantly suppressed tumor growth of HNSCC xenografts and ASCC patient derived xenografts, supporting that DCLK1 is critical for SCC growth.

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Establishment and characterization of the human SaTM-1 anal canal squamous cell carcinoma cell line derived from lymph node metastasis

Cited by 5 publications

References 8 publications

The Human Papillomavirus as a Common Pathogen in Oropharyngeal, Anal and Cervical Cancers

The Human Papillomavirus as a Common Pathogen in Oropharyngeal, Anal and Cervical Cancers

Doublecortin‐like kinase 1 is a therapeutic target in squamous cell carcinoma

Doublecortin-like kinase 1 is a therapeutic target in squamous cell carcinoma

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