Gene expression profiling of bovine ovarian follicular and luteal cells provides insight into cellular identities and functions

Romereim, Sarah M.; Summers, Adam F.; Pohlmeier, William E.; Pan, Zhifang; Hou, Xiaoying; Talbott, Heather; Cushman, Robert A.; Wood, Jennifer R.; Davis, John S.; Cupp, Andrea S.

doi:10.1016/j.mce.2016.09.029

Cited by 43 publications

(55 citation statements)

References 48 publications

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“…It was therefore hypothesized that the upregulation may be associated with the angiogenesis of the CL. A further study conducted by Romereim et al ( 38 ) demonstrated that the CCNl/Cyr61 gene is highly expressed in small luteal cells isolated from cows. These findings support the hypothesis that CCNl/Cyr61 is important in the development of the CL.…”

Section: Ccn1/cyr61 and The Female Reproductive Systemmentioning

confidence: 93%

Biological functions and role of CCN1/Cyr61 in embryogenesis and tumorigenesis in the female reproductive system (Review)

Yang

Chen

2017

Mol Med Report

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Cysteine-rich angiogenic inducer 61 (CCN1/Cyr61) is a prompt response transcription product activated by growth factors. As a member of the CCN family, it mediates cell survival, proliferation, differentiation, migration, adhesion and synthesis of the extracellular matrix by binding directly to the integrins and heparin sulfate proteoglycans or activating multiple signaling transduction pathways. It has previously been demonstrated that CCN1/Cyr61 exhibits an important role in the female reproductive system during embryogenesis and tumorigenesis. However, the functions of CCN1/Cyr61 in the female reproductive system have not been systematically investigated, therefore, the primary aim of the present review is to introduce the role and function of CCN1/Cyr61 in the female reproductive system. The current review presents the molecular structure and biological function of CCN1/Cyr61 and provides detailed data on its expression pattern and contribution to the female reproductive system, including the role in embryogenesis and tumorigenesis.

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Section: Ccn1/cyr61 and The Female Reproductive Systemmentioning

confidence: 93%

Biological functions and role of CCN1/Cyr61 in embryogenesis and tumorigenesis in the female reproductive system (Review)

Yang

Chen

2017

Mol Med Report

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“…representation of components of the IL-33 receptor complex (Romereim et al, 2017;Talbott et al, 2017). In the regressing CL, IL-33 could play a role in macrophage recruitment (Carlock et al, 2014; Wu et al, 2015) and mast cell activation (Lott et al, 2015); and is likely derived from the endothelial cells rather than the steroidogenic cells of the CL (Carlock et al, 2014).…”

Section: Cytokine Signalingmentioning

confidence: 99%

Early transcriptome responses of the bovine midcycle corpus luteum to prostaglandin F2α includes cytokine signaling

Talbott

Hou

Qiu

et al. 2017

Molecular and Cellular Endocrinology

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In ruminants, prostaglandin F2alpha (PGF2α)-mediated luteolysis is essential prior to estrous cycle resumption, and is a target for improving fertility. To deduce early PGF2α-provoked changes in the corpus luteum a short time-course (0.5-4 h) was performed on cows at midcycle. A microarray-determined transcriptome was established and examined by bioinformatic pathway analysis. Classic PGF2α effects were evident by changes in early response genes (FOS, JUN, ATF3) and prediction of active pathways (PKC, MAPK). Several cytokine transcripts were elevated and NF-κB and STAT activation were predicted by pathway analysis. Self-organizing map analysis grouped differentially expressed transcripts into ten mRNA expression patterns indicative of temporal signaling cascades. Comparison with two analogous datasets revealed a conserved group of 124 transcripts similarly altered by PGF2α treatment, which both, directly and indirectly, indicated cytokine activation. Elevated levels of cytokine transcripts after PGF2α and predicted activation of cytokine pathways implicate inflammatory reactions early in PGF2α-mediated luteolysis.

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“…Previous studies and our investigations showed that the NR5A2 , STAR , FGF2 and BMP5 genes might have potential application value in regulating caprine oestrus. Furthermore, we identified cell-type makers and potential lineage markers in follicular development that showed different methylation levels; these markers included cadherin-12 ( CDH12 ), regulators of G-protein signalling ( RGSs ) and NIPA-like protein 3 ( NIPAL3 ) [ 57 ]. In addition, several regions (chromosomes 5 and 17) associated with the same gene or gene cluster ( DGCR6L , HOXC4 , HOXC5 , HOXC6 , HOXC8 , HOXC9 and HOXC10 ) can be differentially methylated (Additional file 4 : Table S4).…”

Section: Discussionmentioning

confidence: 99%

Genome-wide differences in DNA methylation changes in caprine ovaries between oestrous and dioestrous phases

Ping

et al. 2018

J Animal Sci Biotechnol

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BackgroundDNA methylation plays a vital role in reproduction. Entire genome DNA methylation changes during the oestrous phase (ES) and dioestrous phase (DS) in the ovaries of Guanzhong dairy goats were investigated using bisulphite sequencing to understand the molecular biological mechanisms of these goats’ oestrous cycle.ResultsWe discovered distinct genome-wide DNA methylation patterns in ES and DS ovaries. A total of 26,910 differentially methylated regions were upregulated and 21,453 differentially methylated regions were downregulated in the ES samples compared with the DS samples (P-values ≤0.05 and fold change of methylation ratios ≥2). Differentially methylated region analysis showed hypomethylation in the gene body regions and hypermethylation in the joining region between upstream regions and gene bodies. The methylation ratios of the STAR, FGF2, FGF12, BMP5 and SMAD6 genes in the ES samples were lower than those of the DS samples (P-values ≤0.05 and fold change of methylation ratios ≥2). Conversely, the methylation ratios of the EGFR, TGFBR2, IGF2BP1 and MMD2 genes increased in the ES samples compared with the DS samples. In addition, 223 differentially methylated genes were found in the GnRH signalling pathway (KO04912), ovarian steroidogenesis pathway (KO04913), oestrogen signalling pathway (KO04915), oxytocin signalling pathway (KO04921), insulin secretion pathway (KO04911) and MAPK signalling pathway (KO04010).ConclusionsThis study is the first large-scale comparison of the high-resolution DNA methylation landscapes of oestrous and dioestrous ovaries from dairy goats. Previous studies and our investigations have shown that the NR5A2, STAR, FGF2 and BMP5 genes might have potential application value in regulating caprine oestrus.Electronic supplementary materialThe online version of this article (10.1186/s40104-018-0301-x) contains supplementary material, which is available to authorized users.

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Gene expression profiling of bovine ovarian follicular and luteal cells provides insight into cellular identities and functions

Cited by 43 publications

References 48 publications

Biological functions and role of CCN1/Cyr61 in embryogenesis and tumorigenesis in the female reproductive system (Review)

Biological functions and role of CCN1/Cyr61 in embryogenesis and tumorigenesis in the female reproductive system (Review)

Early transcriptome responses of the bovine midcycle corpus luteum to prostaglandin F2α includes cytokine signaling

Genome-wide differences in DNA methylation changes in caprine ovaries between oestrous and dioestrous phases

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