Genome-wide differences in DNA methylation changes in caprine ovaries between oestrous and dioestrous phases

An, Xiaopeng; Ma, Haidong; Ping, Han; Zhu, Chao; Cao, Binyun; Bai, Yueyu

doi:10.1186/s40104-018-0301-x

Cited by 14 publications

(15 citation statements)

References 60 publications

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“…Interestingly, differential methylation of the STAR DNA sequence has been reported in caprine ovaries. The methylation ratio of the STAR gene was lower in the estrus phase compared to the diestrus phase [ 46 ]. On the other hand, no differences in luteal STAR expression on day 12 of the estrous cycle and pregnancy have been observed in previous reports [ 15 ].…”

Section: Discussionmentioning

confidence: 99%

Estradiol-17β Regulates Expression of Luteal DNA Methyltransferases and Genes Involved in the Porcine Corpus Luteum Function In Vivo

Kaczyński

Baryla

Goryszewska

et al. 2021

IJMS

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The corpus luteum (CL) is a temporary endocrine gland vital for pregnancy establishment and maintenance. Estradiol-17β (E2) is the major embryonic signal in pigs supporting the CL’s function. The mechanisms of the luteoprotective action of E2 are still unclear. The present study aimed to determine the effect of E2 on luteal expression of factors involved in CL function. An in vivo model of intrauterine E2 infusions was applied. Gilts on day 12 of pregnancy and the estrous cycle were used as referential groups. Concentrations of E2 and progesterone were elevated in CLs of gilts receiving E2 infusions, compared to placebo-treated gilts. Estradiol-17β stimulated luteal expression of DNA-methyltransferase 1 (DNMT1), but decreased expression of DNMT3B gene and protein, as well as DNMT3A protein. Similar results for DNMT3A and 3B were observed in CLs on day 12 of pregnancy compared to day 12 of the estrous cycle. Intrauterine infusions of E2 altered luteal expression of the genes involved in CL function: PTGFR, PTGES, STAR, HSD17B1, CYP19A1, and PGRMC1. Our findings indicate a role for E2 in expression regulation of factors related to CL function and a novel potential for E2 to regulate DNA methylation as putative physiological mechanisms controlling luteal gene expression.

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Section: Discussionmentioning

confidence: 99%

Estradiol-17β Regulates Expression of Luteal DNA Methyltransferases and Genes Involved in the Porcine Corpus Luteum Function In Vivo

Kaczyński

Baryla

Goryszewska

et al. 2021

IJMS

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“…DNA methylation is an epigenetic modification that regulates gene expression and serves as a key regulator of development (Fan et al, 2020;Yang et al, 2021), differentiation (Khavari et al, 2010), and other processes (Miyakuni et al, 2021). Recent studies have shifted their focus toward how DNA methylation regulates pubertal onset (Lomniczi et al, 2015;Yang et al, 2016;Yuan et al, 2019), estrus (An et al, 2018;Zhou et al, 2018), ovary (Wang et al, 2014;Yu et al, 2015;Sagvekar et al, 2019), oocyte (Mattern et al, 2017;Wei et al, 2019), and embryo development (Salilew-Wondim et al, 2015;Canovas et al, 2017), and reproductive diseases (Vazquez-Martinez et al, 2019;Cao et al, 2021;Zhong et al, 2021). In-depth studies pertaining on epigenetic modifications, such as DNA methylation, are facilitating improvement in animal breeding (Gonzalez-Recio et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Integrative Genome-Wide DNA Methylome and Transcriptome Analysis of Ovaries from Hu Sheep with High and Low Prolific

Yao

Wei³

et al. 2022

Front. Cell Dev. Biol.

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DNA methylation plays an important role in biological processes by affecting gene expression. However, how DNA methylation regulates phenotypic variation in Hu sheep remains unclear. Therefore, we generated genome-wide DNA methylation and transcriptomic profiles in the ovaries of Hu sheep with different prolificacies and genotypes (FecBB and FecB+). Results showed that ovary DNA methylome and transcriptome were significantly different between high prolificacy and low prolificacy Hu sheep. Comparative methylome analyses identified 10,644, 9,594, and 12,214 differentially methylated regions and 87, 1,121, and 2,375 genes, respectively, showing differential expression levels in three different comparison groups. Female reproduction-associated differentially methylated regions-related genes and differentially expressed genes were enriched, thereby the respective interaction networks were constructed. Furthermore, systematical integrative analyses revealed a negative correlation between DNA methylation around the transcriptional start site and gene expression levels, which was confirmed by testing the expression of integrin β2 subunit (ITGB2) and lysosome-associated protein transmembrane-4 beta (LAPTM4B) in vivo and in vitro. These findings demonstrated that DNA methylation influences the propensity for prolificacy by affecting gene expression in the ovaries, which may contribute to a greater understanding of the epigenome and transcriptome that will be useful for animal breeding.

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“…DNA methylation plays an important role in regulating the expression of gene transcription, and methylation can also lead to long-term alterations in gene expression (Falck et al 2012;Feinberg 2007). DNA methylation is critical to reproduction (An et al 2018). DNA methylation is catalyzed by the DNA methyltransferase (DNMT) family that includes three main members, DNMT1, DNMT3a, and DNMT3b.…”

Section: Introductionmentioning

confidence: 99%

DNA Methylation may be a testicular plateau adaptation in Tibetan pig

Zhao

Tian

Cheng

et al. 2021

Journal of Applied Animal Research

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DNA methylation plays an important role in reproduction. Tibetan pigs maintain high sperm motility, even under extreme conditions at high altitudes. However, the DNA methylation mechanisms underlying testicular plateau adaptations in Tibetan pigs remain unclear. We determined the genomic DNA methylation level in the testicular tissues of Tibetan pigs living at high altitudes (TP) and of Yorkshire pigs that migrated to high altitudes (YP) using the fluorescence method. Quantitative realtime PCR and western blots were used to measure the gene and protein expression levels, respectively, of three DNA methyltransferases (DNMT1, DNMT3A, and DNMT3B) and hypoxia-inducible factor 2α (HIF2α). We found that the genomic DNA methylation level and the mRNA and protein expression levels of the four genes were all significantly lower in the testicular tissues of TP than YP. In short, compared with YP, the downregulation of DNA methyltransferases in the TP testes led to lower levels of genomic DNA methylation, which would facilitate higher sperm motility. Meanwhile, HIF2α expression in the TP testes was down-regulated, which might contributes to TP testes' adaption to the plateau environment. This study provided new insights into the DNA methylation mechanisms underlying plateau adaptations in TP testicles.

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Genome-wide differences in DNA methylation changes in caprine ovaries between oestrous and dioestrous phases

Cited by 14 publications

References 60 publications

Estradiol-17β Regulates Expression of Luteal DNA Methyltransferases and Genes Involved in the Porcine Corpus Luteum Function In Vivo

Estradiol-17β Regulates Expression of Luteal DNA Methyltransferases and Genes Involved in the Porcine Corpus Luteum Function In Vivo

Integrative Genome-Wide DNA Methylome and Transcriptome Analysis of Ovaries from Hu Sheep with High and Low Prolific

DNA Methylation may be a testicular plateau adaptation in Tibetan pig

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