Gene expression profile changes in the jejunum of weaned piglets after oral administration of Lactobacillus or an antibiotic

Zhang, Dongyan; Shang, Tingting; Huang, Yan; Wang, Sixin; Liu, Hui; Wang, Jing; Wang, Yamin; Ji, Hai‐Feng; Zhang, Rijun

doi:10.1038/s41598-017-16158-y

Cited by 16 publications

(9 citation statements)

References 46 publications

(40 reference statements)

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“…Moreover, an analysis of the LCFAs profiles of piglets also revealed that the PUFAs C18:2n6c, C18:3n3, C20:4n6, and C22:6n3 were significantly higher in the L. reuteri ZLR003 group compared with the control group. This is consistent with our finding from a previous study that the PUFAs C18:2n6c, C18:3n3, and C20:4n6 in the colon were significantly higher in the L. reuteri group compared with the control group (Zhang D.Y. et al, 2017).…”

Section: Discussionsupporting

confidence: 94%

“…Our previous work showed that this strain affected the gut microbiota composition in the jejunum, colon, and cecum of weaned piglets (Zhang et al, 2016). In addition, the results of Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis by RNA-seq in the jejunum tissue showed that the expression of genes related to arachidonic acid metabolism and linoleic acid metabolism, glutathione metabolism, glycine, and serine and threonine metabolism were improved after treatment with L. reuteri ZLR003 compared with the control group (Zhang D.Y. et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Fecal Microbiota and Its Correlation With Fatty Acids and Free Amino Acids Metabolism in Piglets After a Lactobacillus Strain Oral Administration

et al. 2019

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Lactobacillus has a positive effect on the host intestinal microbiota. In piglets, dietary supplementation with Lactobacillus affects general health and plays an important role in nutrient digestion and fermentation. However, this association requires further investigation. Here, we studied newborn piglets from 12 litters. The nursed piglets were given a creep feed beginning on day 10 post-partum and weaned at day 30. Piglets were fed either a control basic diet or a diet including supplementation with Lactobacillus reuteri ZLR003 at 6.0 × 10 6 CFU/g feed. At day 30 and 60, feces samples were taken and used for sequencing of the V3-V4 hypervariable region of the 16S rRNA gene. At day 60, feces samples and serum samples were also taken and used to measure the short chain fatty acids (SCFAs) and to detect long chain fatty acids (LCFAs) and free amino acids (FAAs), respectively. The results revealed that L. reuteri ZLR003 could improve piglet fecal microbiota composition, especially at the end of weaned period. The concentrations of lactic acid and butyric acid in feces were higher, and acetic acid concentration was lower in the L. reuteri ZLR003 group compared with the control group ( P < 0.05). The serum polyunsaturated fatty acids C18:2n6c, C18:3n3, C20:4n6, and C22:6n3 were significantly higher ( P < 0.05), as were the serum FAAs Gly, Ala, Val, Iso, Asn, Asp, Glu, Met, Phe, and Leu ( P < 0.05), in the L. reuteri group compared with the control group. A correlation analysis revealed that the genera Ruminococcaceae _UCG-010 and Ruminococcaceae _UCG-014 had a negative correlation with the SCFAs content in feces, the genus Prevotella _9 had a higher positive correlation with C18:2n6c, and the genera Megasphaera and Mitsuokella had a more positive significant effect on the serum FAAs content in weaned piglets in the L. reuteri ZLR003 group compared with the control group. In conclusion, L. reuteri ZLR003 influenced the fecal microbiota composition of piglets, and its effects were related to the metabolism of SCFAs, LCFAs, and FAAs. Our findings will help facilitate the application of Lactobacillus strains in pig production.

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Section: Discussionsupporting

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Fecal Microbiota and Its Correlation With Fatty Acids and Free Amino Acids Metabolism in Piglets After a Lactobacillus Strain Oral Administration

et al. 2019

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“…This observation is consistent with the previously reported ability of lactobacilli to induce Nrf2-ARE and GPX2 expression. Such effect was observed both in vitro in mouse embryotic fibroblast and NIT-3T3 cells, using Lactobacillus gasseri or metabolites from L. plantarum on HepG2 cells, and in vivo in pig’s jejunum, using Lactobacillus reuteri ( 50 – 52 ). Another in vivo study conducted with diabetic patients demonstrated that consumption of yogurt containing probiotics ( Lactobacillus acidophilus La5 and Bifidobacterium lactis Bb12) for 6 weeks was able to influence the oxidative status in type 2 diabetic patients, inducing decreased fasting blood glucose and glycated hemoglobin and increased activities of SOD and GPX2, and total antioxidant status compared with the control yogurt group without probiotics ( 53 ).…”

Section: Discussionmentioning

confidence: 99%

Redox Role of Lactobacillus casei Shirota Against the Cellular Damage Induced by 2,2′-Azobis (2-Amidinopropane) Dihydrochloride-Induced Oxidative and Inflammatory Stress in Enterocytes-Like Epithelial Cells

et al. 2018

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In western societies where most of the day is spent in the postprandial state, the existence of oxidative and inflammatory stress conditions makes postprandial stress an important factor involved in the development of cardiovascular risk factors. A large body of evidence have been accumulated on the anti-inflammatory effects of probiotics, but no information is available on the mechanisms through which intestinal microbiota modulates redox unbalance associated with inflammatory stress. Here, we aimed to investigate the ability of Lactobacillus casei Shirota (LS) to induce an antioxidant response to counteract oxidative and inflammatory stress in an in vitro model of enterocytes. Our results show that pretreatment of enterocytes with LS prevents membrane barrier disruption and cellular reactive oxygen species (ROS) accumulation inside the cells, modulates the expression of the gastro-intestinal glutathione peroxidase (GPX2) antioxidant enzyme, and reduces p65 phosphorylation, supporting the involvement of the Nfr2 and nuclear factor kappa B pathways in the activation of antioxidant cellular defenses by probiotics. These results suggest, for the first time, a redox mechanism by LS in protecting intestinal cells from AAPH-induced oxidative and inflammatory stress.

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“…The gastrointestinal tract is susceptible to various stresses, such as weaning in early life and challenges with pathogenic bacteria or toxins. Because intestinal dysfunction caused by various stresses is directly related to growth performance in pigs, affecting parameters such as feed intake, average daily gain, and gain/feed ratio, it is important to consider a variety of environmental factors, including diets and stressors, to maintain intestinal health 10,11 . The fact that bioactive food compounds in the diet can directly interact with genes and affect transcription factors, protein expression, and metabolite production has led to nutritional research in livestock 12 .…”

Section: Discussionmentioning

confidence: 99%

N-acetylcysteine modulates lipopolysaccharide-induced intestinal dysfunction

Lee

Kang²

2019

Sci Rep

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The gastrointestinal epithelium functions in nutrient absorption and pathogens barrier and its dysfunction directly affects livestock performance. N-Acetylcysteine (NAC) improves mucosal function, but its effects on intestinal functions at the molecular level remain unclear. Here, we performed gene expression profiling of the pig small intestine after dietary NAC treatment under LPS challenge and investigated the effects of NAC on intestinal epithelial cells in vitro. Dietary NAC supplementation under LPS challenge altered the small intestine expression of 959 genes related to immune response, inflammatory response, oxidation-reduction process, cytokine-cytokine receptor interaction, and the cytokine-mediated signalling, Toll-like receptor signalling pathway, Jak-STAT signalling pathway, and TNF signalling pathway. We then analysed the expression patterns of the top 10 altered genes, and found that NAC markedly stimulated HMGCS3 and LDHC expression in IPEC-J2 cells. In vitro, NAC pre-treatment significantly reduced TNF-α and NF-κB, TNF-α, IFN-γ, and IL-6 expression in LPS-induced IPEC-J2 cells. NAC treatment also significantly reduced oxidative stress in LPS-induced IPEC-J2 cells and alleviated intestinal barrier function and wound healing. Thus, NAC as a feed additive can enhance livestock intestinal health by modulating intestinal inflammation, permeability, and wound healing under LPS-induced dysfunction, improving our molecular understanding of the effects of NAC on the intestine.

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Gene expression profile changes in the jejunum of weaned piglets after oral administration of Lactobacillus or an antibiotic

Cited by 16 publications

References 46 publications

Fecal Microbiota and Its Correlation With Fatty Acids and Free Amino Acids Metabolism in Piglets After a Lactobacillus Strain Oral Administration

Fecal Microbiota and Its Correlation With Fatty Acids and Free Amino Acids Metabolism in Piglets After a Lactobacillus Strain Oral Administration

Redox Role of Lactobacillus casei Shirota Against the Cellular Damage Induced by 2,2′-Azobis (2-Amidinopropane) Dihydrochloride-Induced Oxidative and Inflammatory Stress in Enterocytes-Like Epithelial Cells

N-acetylcysteine modulates lipopolysaccharide-induced intestinal dysfunction

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