Gene-activated titanium implants for gene delivery to enhance osseointegration

Wang, Zhikang; Zhang, Jing; Hu, Jinxing; Yang, Guoli

doi:10.1016/j.bioadv.2022.213176

Cited by 5 publications

(2 citation statements)

References 162 publications

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“…The success rate of Ti dental implant surgery can reach over 90%, but there are still about 10% failures . The key that determines the success of Ti implants is the effective osseointegration. − In addition, improving the antibacterial properties of the implant and suppressing peri-implant inflammation are also important factors for the success of the surgery. − Osteogenesis requires osteoblasts to adhere and grow on the surface of the implant. − And the occurrence of inflammation is mostly related to bacterial adsorption and biofilm formation on the surface of implants. − All of the above processes are closely related to the surface conditions of Ti implants. However, during the Ti implant manufacturing process, if the overall material is changed to achieve good surface conditions, the overall performance of the implant may be seriously affected.…”

Section: Introductionmentioning

confidence: 99%

Surface Modification and Functionalities for Titanium Dental Implants

Sun,

Liu,

Wang

et al. 2023

ACS Biomater. Sci. Eng.

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Dental implants have become the mainstream strategy for oral restoration, and implant materials are the most important research hot spot in this field. So far, Ti implants dominate all kinds of implants. The surface properties of the Ti implant play decisive roles in osseointegration and antibacterial performance. Surface modifications can significantly change the surface micro/nanotopography and composition of Ti implants, which will effectively improve their hydrophilicity, mechanical properties, osseointegration performance, antibacterial performance, etc. These optimizations will thus improve implant success and service life. In this paper, the latest surface modification techniques of Ti dental implants are systematically and comprehensively reviewed. The various biomedical functionalities of surface modifications are discussed in-depth. Finally, a profound comment on the challenges and opportunities of this frontier is proposed, and the most promising directions for the future were explored.

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Section: Introductionmentioning

confidence: 99%

Surface Modification and Functionalities for Titanium Dental Implants

Sun,

Liu,

Wang

et al. 2023

ACS Biomater. Sci. Eng.

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“…As an alternative, gene-activated matrix (GAM) coating incorporated with osteogenic genes (such as pBMP2 [ 7 , 8 ], miRNA [ 9 , 10 ], and siCkip-1 [ 11 ]) highly improved osseointegration of implants due to prolonged transgene activity and better immune compatibility. Unfortunately, the poor delivery efficiency on the hard-to-transfect targeting cells caused by the helpless delivery vector, limited gene loading and uncontrolled release is still blocked in preclinical studies [ 12 ]. More importantly, the interaction between carrier system and implant biophysical cues was rarely explored, especially for further application in vivo practical conditions.…”

Section: Introductionmentioning

confidence: 99%

Fibrous topology promoted pBMP2-activated matrix on titanium implants boost osseointegration

He,

Wang,

Wang

et al. 2023

Regenerative Biomaterials

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Titanium (Ti) implants have been extensively used after surgical operations. Its surface bioactivity is of importance to facilitate integration with surrounding bone tissue, and ultimately ensure stability and long-term functionality of the implant. The plasmid DNA-activated matrix (DAM) coating on the surface could benefit osseointegration but is still trapped by poor transfection for further application, especially on the bone marrow mesenchymal stem cells (BMSCs) in vivo practical conditions. Herein, we constructed a DAM on the surface of fibrous-grained titanium (FG Ti) composed of phase-transition lysozyme (P) as adhesive, cationic arginine-rich lipid (RLS) as the transfection agent and plasmid DNA (pDNA) for bone morphology protein 2 (BMP2) expression. The cationic lipid RLS improved up to 30-fold higher transfection than that of commercial reagents (Lipofectamine 2000 and polyethyleneimine) on MSC. And importantly, Ti surface topology not only promotes the DAM to achieve high transfection efficiency (∼75.7% positive cells) on MSC due to the favorable combination but also reserves its contact induction effect for osteoblasts. Upon further exploration, the fibrous topology on FG Ti could boost pDNA uptake for gene transfection, and cell migration in MSC through cytoskeleton remodeling and induce contact guidance for enhanced osteointegration. At the same time, the cationic RLS together with adhesive P were both antibacterial, showing up to 90% inhibition rate against Escherichia coli and Staphylococcus aureus with reduced adherent microorganisms and disrupted bacteria. Finally, the FG Ti-P/pBMP2 implant achieved accelerated bone healing capacities through highly efficient gene delivery, aligned surface topological structure and increased antimicrobial properties in a rat femoral condylar defect model.

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Urolithin B inhibits the differentiation of M1 macrophages and relieves the inflammation around the implants under osteoporosis via down-regulating the phosphorylation of VEGFR2

Xiao,

Yang,

et al. 2024

International Immunopharmacology

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Gene-activated titanium implants for gene delivery to enhance osseointegration

Cited by 5 publications

References 162 publications

Surface Modification and Functionalities for Titanium Dental Implants

Surface Modification and Functionalities for Titanium Dental Implants

Fibrous topology promoted pBMP2-activated matrix on titanium implants boost osseointegration

Urolithin B inhibits the differentiation of M1 macrophages and relieves the inflammation around the implants under osteoporosis via down-regulating the phosphorylation of VEGFR2

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