Gelatin-alginate coacervates for circumventing proteolysis and probing intermolecular interactions by SPR

Lau, Hui-Chong; Jeong, Seonghee; Kim, Aeri

doi:10.1016/j.ijbiomac.2018.05.093

Cited by 11 publications

(15 citation statements)

References 42 publications

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“…Among all the tested compositions, the LWGA-SA (1:0.4) at pH 4.34 condition resulted in the highest EGF encapsulation efficiency. In our previous study, coacervates composed of several ratios of HWGA-SA (3:1, 4:1, 5:1, and 6:1) titrated to pH 5.0 effectively encapsulated BSA but not EGF [17]. Further optimization using various polymer ratios of either HWGA and SA or HWGA and hyaluronic acid, at various salt concentration did not improve the encapsulation efficiency of EGF.…”

Section: Resultsmentioning

confidence: 99%

“…In our previous study, coacervates composed of high molecular weight Type A gelatin (HWGA) and sodium alginate (SA) showed high encapsulation efficiency for bovine serum albumin (BSA), protecting BSA from trypsin digestion. However, encapsulation of epidermal growth factor (EGF) was not successful in the HWGA-SA coacervates [17]. The difference in the encapsulation efficiency between BSA and EGF in HWGA-SA coacervates was attributed to the different level of binding of BSA and EGF to HWGA; the surface plasmon resonance analysis revealed high affinity of BSA to HWGA, but no significant binding of EGF to HWGA was detected.…”

Section: Introductionmentioning

confidence: 99%

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Gelatin-Alginate Complexes for EGF Encapsulation: Effects of H-Bonding and Electrostatic Interactions

et al. 2019

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Gelatin Type A (GA) and sodium alginate (SA) complexes were explored to encapsulate epidermal growth factor (EGF), and thereby to circumvent its proteolytic degradation upon topical application to chronic wounds. Phase diagrams were constructed based on turbidity as a function of GA to SA ratio and pH. Various GA-SA mixtures were compared for polydispersity index, zeta potential, Z-average, and ATR-FTIR spectra. Trypsin digestion and human dermal fibroblast scratch wound assay were done to evaluate the effects of EGF encapsulation. The onset pH values for coacervation and precipitation were closer together in high molecular weight GA (HWGA)-SA reaction mixtures than in low molecular weight GA (LWGA)-SA, which was attributed to strong H-bonding interactions between HWGA and SA probed by ATR-FTIR. EGF incorporation in both HWGA-SA precipitates and LWGA-SA coacervates below the isoelectric point of EGF, but not above it, suggests the contribution of electrostatic interactions between EGF and SA. EGF encapsulated in LWGA-SA coacervates was effectively protected from trypsin digestion and showed better in vitro scratch wound activity compared to free EGF. LWGA-SA coacervates are suggested as a novel delivery system for topical application of EGF to chronic wounds.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Gelatin-Alginate Complexes for EGF Encapsulation: Effects of H-Bonding and Electrostatic Interactions

et al. 2019

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“…Coacervates composed of high molecular weight gelatin A and sodium alginate (HWGA-SA) showed high encapsulation efficiency for bovine serum albumin (BSA), protecting it from trypsin digestion. However, the encapsulation of EGF was not successful in the same coacervation condition [16]. Formulation screening for coacervates using GA and sodium alginate (SA) was done for EGF encapsulation, by varying the molecular weight of GA, the ratio of GA to SA, and the reaction pH [17].…”

Section: Introductionmentioning

confidence: 99%

Improved Diabetic Wound Healing by EGF Encapsulation in Gelatin-Alginate Coacervates

et al. 2020

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Topical imageplication of epidermal growth fctor (EGF) has been used to accelerate diabetic foot ulcers but with limited efficacy. In this study, we selected a complex coacervate (EGF-Coa) composed of the low molecular weight gelatin type A and sodium alginate as a novel delivery system for EGF, based on encapsulation efficiency and protection of EGF from protease. EGF-Coa enhanced in vitro migration of keratinocytes and accelerated wound healing in streptozotocin-induced diabetic mice with increased granulation and re-epithelialization. While diabetic wound sites without treatment showed downward growth of hyperproliferative epidermis along the wound edges with poor matrix formation, EGF-Coa treatment recovered horizontal migration of epidermis over the newly deposited dermal matrix. EGF-Coa treatment also resulted in reduced levels of proinflammatory cytokines IL-1, IL-6, and THF-α. Freeze-dried coacervates packaged in aluminum pouches were stable for up to 4 months at 4 and 25 °C in terms of appearance, purity by RP-HPLC, and in vitro release profiles. There were significant physical and chemical changes in relative humidity above 33% or at 37 °C, suggesting the requirement for moisture-proof packaging and cold chain storage for long term stability. We propose low molecular weight gelatin type A and sodium alginate (LWGA-SA) coacervates as a novel EGF delivery system with enhanced efficacy for chronic wounds.

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“…In this regard, the chemical modification of Gel using stimuli-responsive synthetic polymers and its crosslinking can be considered as powerful approaches to the development of "smart" DDSs based on this natural polypeptide. [15][16][17][18] In the last few years, numerous DDSs have been designed and engineered to respond to various internal or external stimuli. [19][20][21] Amongst, pH-and thermal-responsive DDSs have received more attention owing to their simplicities as well as abnormal microenvironment condition of tumoral tissues (lower pH and higher temperature values) in comparison with healthy tissues that resulted to targeted release of encapsulated cargo.…”

Section: Introductionmentioning

confidence: 99%

“…However, Gel has poor mechanical strength, high solubility, and somewhat high degradation rate that can be modified through various chemical or physical techniques. In this regard, the chemical modification of Gel using stimuli‐responsive synthetic polymers and its crosslinking can be considered as powerful approaches to the development of “smart” DDSs based on this natural polypeptide 15–18 …”

Section: Introductionmentioning

confidence: 99%

A bio‐inspired gelatin‐based pH‐ and thermal‐sensitive magnetic hydrogel for in vitro chemo/hyperthermia treatment of breast cancer cells

Derakhshankhah

Jahanban‐Esfahlan

Vandghanooni

et al. 2021

J of Applied Polymer Sci

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Gelatin (Gel)-based pH-and thermal-responsive magnetic hydrogels (MH-1 and MH-2) were designed and developed as novel drug delivery systems (DDSs) for cancer chemo/hyperthermia therapy. For this goal, Gel was functionalized with methacrylic anhydride (GelMA), and then copolymerized with (2-dimethylaminoethyl) methacrylate (DMAEMA) monomer in the presence of methacrylate-end capped magnetic nanoparticles (MNPs) as well as triethylene glycol dimethacrylate (TEGDMA; as crosslinker). Afterward, a thiolend capped poly(N-isopropylacrylamide) (PNIPAAm-SH) was synthesized through an atom transfer radical polymerization technique, and then attached onto the hydrogel through "thiol-ene" click grafting. The preliminary performances of developed MHs for chemo/hyperthermia therapy of human breast cancer was investigated through the loading of doxorubicin hydrochloride (Dox) as an anticancer agent followed by cytotoxicity measurement of drug-loaded DDSs using MTT assay by both chemo-and chemo/hyperthermia-therapies. Owing to porous morphologies of the fabricated magnetic hydrogels according to scanning electron microscopy images and strong physicochemical interactions (e.g., hydrogen bonding) the drug loading capacities of the MH-1 and MH-2 were obtained as 72 ± 1.4 and 77 ± 1.8, respectively. The DDSs exhibited acceptable pH-and thermal-triggered drug release behaviors. The MTT assay

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Gelatin-alginate coacervates for circumventing proteolysis and probing intermolecular interactions by SPR

Cited by 11 publications

References 42 publications

Gelatin-Alginate Complexes for EGF Encapsulation: Effects of H-Bonding and Electrostatic Interactions

Gelatin-Alginate Complexes for EGF Encapsulation: Effects of H-Bonding and Electrostatic Interactions

Improved Diabetic Wound Healing by EGF Encapsulation in Gelatin-Alginate Coacervates

A bio‐inspired gelatin‐based pH‐ and thermal‐sensitive magnetic hydrogel for in vitro chemo/hyperthermia treatment of breast cancer cells

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