Gelatin-Alginate Complexes for EGF Encapsulation: Effects of H-Bonding and Electrostatic Interactions

Jeong, Seonghee; Kim, Byungwook; Lau, Hui-Chong; Kim, Aeri

doi:10.3390/pharmaceutics11100530

Cited by 17 publications

(24 citation statements)

References 38 publications

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“…The opposite charge of polyanionic polysaccharide chains and positively charged gelatin could electrostatically attract each other, leading to the formation of stable polyelectrolyte complexes [ 18 ]. These findings were consistent with the previous study on complex formation between oppositely charged macromolecules [ 26 ]. The amino groups of gelatin and carboxyl groups of alginate had electrostatic interactions with each other.…”

Section: Discussionsupporting

confidence: 94%

Curcumin-Loaded Bacterial Cellulose/Alginate/Gelatin as A Multifunctional Biopolymer Composite Film

et al. 2020

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Multifunctional biopolymer composites comprising mechanically-disintegrated bacterial cellulose, alginate, gelatin and curcumin plasticized with glycerol were successfully fabricated through a simple, facile, cost-effective mechanical blending and casting method. SEM images indicate a well-distributed structure of the composites. The water contact angles existed in the range of 50–70°. Measured water vapor permeability values were 300–800 g/m2/24 h, which were comparable with those of commercial dressing products. No release of curcumin from the films was observed during the immersion in PBS and artificial saliva, and the fluid uptakes were in the range of 100–700%. Films were stretchable and provided appropriate stiffness and enduring deformation. Hydrated films adhered firmly onto the skin. In vitro mucoadhesion time was found in the range of 0.5–6 h with porcine mucosa as model membrane under artificial saliva medium. The curcumin-loaded films had substantial antibacterial activity against E. coli and S. aureus. The films showed non-cytotoxicity to human keratinocytes and human gingival fibroblasts but exhibited potent anticancer activity in oral cancer cells. Therefore, these curcumin-loaded films showed their potential for use as leave-on skin applications. These versatile films can be further developed to achieve desirable characteristics for local topical patches for wound care, periodontitis and oral cancer treatment.

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Section: Discussionsupporting

confidence: 94%

Curcumin-Loaded Bacterial Cellulose/Alginate/Gelatin as A Multifunctional Biopolymer Composite Film

et al. 2020

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“…Another strategy to synthesize particles relies on complexation, based on the electrostatic interactions between alginate at neutral and alkaline pH values, bioactive agents, and other kinds of naturally occurring polymers, such as the polycation chitosan [ 147 – 149 ].…”

Section: Various Techniques To Produce Alginate Micro/nanoparticlementioning

confidence: 99%

Current Status of Alginate in Drug Delivery

Hariyadi

Islam

2020

Advances in Pharmacological and Pharmaceutical Sciences

130

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Alginate is one of the natural polymers that are often used in drug- and protein-delivery systems. The use of alginate can provide several advantages including ease of preparation, biocompatibility, biodegradability, and nontoxicity. It can be applied to various routes of drug administration including targeted or localized drug-delivery systems. The development of alginates as a selected polymer in various delivery systems can be adjusted depending on the challenges that must be overcome by drug or proteins or the system itself. The increased effectiveness and safety of sodium alginate in the drug- or protein-delivery system are evidenced by changing the physicochemical characteristics of the drug or proteins. In this review, various routes of alginate-based drug or protein delivery, the effectivity of alginate in the stem cells, and cell encapsulation have been discussed. The recent advances in the in vivo alginate-based drug-delivery systems as well as their toxicities have also been reviewed.

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“…Among the two compositions of coacervates previously studied, LWGA-SA (1:0.4) was selected for the present study, based on its higher encapsulation efficiency and better protection from trypsin digestion compared to LWGA-SA (1:1) [17]. Briefly, EGF-Coa was prepared by mixing the aqueous solutions of LWGA (1%, w/w) and SA (0.5%, w/w) to (1:0.4), at a total polymer concentration of 5 mg/g, followed by addition of an aliquot of EGF solution, and then finally an aliquot of acetic acid as a pH modifier.…”

Section: Fabrication and Characterization Of Lwga-sa Coacervates Encapsulating Egf (Egf-coa)mentioning

confidence: 99%

“…LWGA-SA physical mixture (1:0.4) containing the same amount of EGF (EGF-PM) was prepared with the same procedure without the acid titration step. Characterization of the samples is described in the previous paper [17]. Samples were freeze-dried for the in vitro release, in vitro activity, and in vivo tests.…”

Section: Fabrication and Characterization Of Lwga-sa Coacervates Encapsulating Egf (Egf-coa)mentioning

confidence: 99%

“…However, the encapsulation of EGF was not successful in the same coacervation condition [16]. Formulation screening for coacervates using GA and sodium alginate (SA) was done for EGF encapsulation, by varying the molecular weight of GA, the ratio of GA to SA, and the reaction pH [17]. As previously reported by others, the molecular weight of the polymers was one of the crucial variables [18][19][20].…”

Section: Introductionmentioning

confidence: 95%

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Improved Diabetic Wound Healing by EGF Encapsulation in Gelatin-Alginate Coacervates

et al. 2020

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Topical imageplication of epidermal growth fctor (EGF) has been used to accelerate diabetic foot ulcers but with limited efficacy. In this study, we selected a complex coacervate (EGF-Coa) composed of the low molecular weight gelatin type A and sodium alginate as a novel delivery system for EGF, based on encapsulation efficiency and protection of EGF from protease. EGF-Coa enhanced in vitro migration of keratinocytes and accelerated wound healing in streptozotocin-induced diabetic mice with increased granulation and re-epithelialization. While diabetic wound sites without treatment showed downward growth of hyperproliferative epidermis along the wound edges with poor matrix formation, EGF-Coa treatment recovered horizontal migration of epidermis over the newly deposited dermal matrix. EGF-Coa treatment also resulted in reduced levels of proinflammatory cytokines IL-1, IL-6, and THF-α. Freeze-dried coacervates packaged in aluminum pouches were stable for up to 4 months at 4 and 25 °C in terms of appearance, purity by RP-HPLC, and in vitro release profiles. There were significant physical and chemical changes in relative humidity above 33% or at 37 °C, suggesting the requirement for moisture-proof packaging and cold chain storage for long term stability. We propose low molecular weight gelatin type A and sodium alginate (LWGA-SA) coacervates as a novel EGF delivery system with enhanced efficacy for chronic wounds.

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Gelatin-Alginate Complexes for EGF Encapsulation: Effects of H-Bonding and Electrostatic Interactions

Cited by 17 publications

References 38 publications

Curcumin-Loaded Bacterial Cellulose/Alginate/Gelatin as A Multifunctional Biopolymer Composite Film

Curcumin-Loaded Bacterial Cellulose/Alginate/Gelatin as A Multifunctional Biopolymer Composite Film

Current Status of Alginate in Drug Delivery

Improved Diabetic Wound Healing by EGF Encapsulation in Gelatin-Alginate Coacervates

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