Gelatin—alginate—cerium oxide nanocomposite scaffold for bone regeneration

Purohit, Shiv Dutt; Singh, Hemant; Bhaskar, Rakesh; Yadav, Indu; Chou, Chia-Fu; Gupta, Mukesh Kumar; Mishra, Narayan Chandra

doi:10.1016/j.msec.2020.111111

Cited by 97 publications

(64 citation statements)

References 66 publications

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“…3 ). This is consistent with the previous studies that CeO 2 NPs could promote proliferation of stem cells, and played a positive role in new bone regeneration when combined with mesoporous bio-glass scaffolds [ 52 ] and gelatin-alginate scaffolds [ 53 ]. Ceria nanoparticles have been reported to promote hypertrophic differentiation of BMSCs via DHX15 activation on bone regeneration enhancement [ 54 ].…”

Section: Discussionsupporting

confidence: 93%

Cerium oxide nanoparticles loaded nanofibrous membranes promote bone regeneration for periodontal tissue engineering

Ren

Zhou

Zheng

et al. 2022

Bioactive Materials

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Bone regeneration is a crucial part in the treatment of periodontal tissue regeneration, in which new attempts come out along with the development of nanomaterials. Herein, the effect of cerium oxide nanoparticles (CeO 2 NPs) on the cell behavior and function of human periodontal ligament stem cells (hPDLSCs) was investigated. Results of CCK-8 and cell cycle tests demonstrated that CeO 2 NPs not only had good biocompatibility, but also promoted cell proliferation. Furthermore, the levels of alkaline phosphatase activity, mineralized nodule formation and expressions of osteogenic genes and proteins demonstrated CeO 2 NPs could promote osteogenesis differentiation of hPDLSCs. Then we chose electrospinning to fabricate fibrous membranes containing CeO 2 NPs. We showed that the composite membranes improved mechanical properties as well as realized release of CeO 2 NPs. We then applied the composite membranes to in vivo study in rat cranial defect models. Micro-CT and histopathological evaluations revealed that nanofibrous membranes with CeO 2 NPs further accelerated new bone formation. Those exciting results demonstrated that CeO 2 NPs and porous membrane contributed to osteogenic ability, and CeO 2 NPs contained electrospun membrane may be a promising candidate material for periodontal bone regeneration.

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Section: Discussionsupporting

confidence: 93%

Cerium oxide nanoparticles loaded nanofibrous membranes promote bone regeneration for periodontal tissue engineering

Ren

Zhou

Zheng

et al. 2022

Bioactive Materials

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“…Purohit et al mixed nano-cerium oxide (Nanoceria, NC) into gelatin-alginate (GA) scaffolds and obtained nano-composite scaffolds (GA-NCs) by freeze-drying. NC has good free radical scavenging ability [ 130 ], the results showed that the addition of NC increased the mechanical properties and biomineralization of the scaffold, and reduced the expansion and weight loss of the scaffold. The synergistic fusion of nanoparticles and GA scaffolds enhanced the adhesion, proliferation and activity of cells, making GA-NCS scaffolds have the potential to assist the differentiation of mesenchymal stem cells (MSCs) into osteoblasts, and have a certain ability of free radical scavenging.…”

Section: Biomedical Applicationsmentioning

confidence: 99%

Preparation of Alginate-Based Biomaterials and Their Applications in Biomedicine

2021

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Alginates are naturally occurring polysaccharides extracted from brown marine algae and bacteria. Being biocompatible, biodegradable, non-toxic and easy to gel, alginates can be processed into various forms, such as hydrogels, microspheres, fibers and sponges, and have been widely applied in biomedical field. The present review provides an overview of the properties and processing methods of alginates, as well as their applications in wound healing, tissue repair and drug delivery in recent years.

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“…The degradation rates of the scaffolds should match the regeneration rates of the host tissues to achieve proper regeneration [40]. In addition, the mechanical integrity of the scaffolds during degradation should be sustainable to allow cellular adhesion and proliferation [41]. In addition, various cellular behaviors require nutrients and growth factors to engage in cell-to-cell interaction in the natural extracellularlike environment provided by the scaffolds.…”

Section: Effects Of Degradation Properties On the Soaking Experimentsmentioning

confidence: 99%

Bidirectional Differentiation of Human-Derived Stem Cells Induced by Biomimetic Calcium Silicate-Reinforced Gelatin Methacrylate Bioink for Odontogenic Regeneration

et al. 2021

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Tooth loss or damage is a common problem affecting millions of people worldwide, and it results in significant impacts on one’s quality of life. Dental regeneration with the support of stem cell-containing scaffolds has emerged as an alternative treatment strategy for such cases. With this concept in mind, we developed various concentrations of calcium silicate (CS) in a gelatin methacryloyl (GelMa) matrix and fabricated human dental pulp stem cells (hDPSCs)-laden scaffolds via the use of a bioprinting technology in order to determine their feasibility in promoting odontogenesis. The X-ray diffraction and Fourier transform-infrared spectroscopy showed that the incorporation of CS increased the number of covalent bonds in the GelMa hydrogels. In addition, rheological analyses were conducted for the different concentrations of hydrogels to evaluate their sol–gel transition temperature. It was shown that incorporation of CS improved the printability and printing quality of the scaffolds. The printed CS-containing scaffolds were able to release silicate (Si) ions, which subsequently significantly enhanced the activation of signaling-related markers such as ERK and significantly improved the expression of odontogenic-related markers such as alkaline phosphatase (ALP), dentin matrix protein-1 (DMP-1), and osteocalcin (OC). The calcium deposition assays were also significantly enhanced in the CS-containing scaffold. Our results demonstrated that CS/GelMa scaffolds were not only enhanced in terms of their physicochemical behaviors but the odontogenesis of the hDPSCs was also promoted as compared to GelMa scaffolds. These results demonstrated that CS/GelMa scaffolds can serve as cell-laden materials for future clinical applications and use in dentin regeneration.

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Gelatin—alginate—cerium oxide nanocomposite scaffold for bone regeneration

Cited by 97 publications

References 66 publications

Cerium oxide nanoparticles loaded nanofibrous membranes promote bone regeneration for periodontal tissue engineering

Cerium oxide nanoparticles loaded nanofibrous membranes promote bone regeneration for periodontal tissue engineering

Preparation of Alginate-Based Biomaterials and Their Applications in Biomedicine

Bidirectional Differentiation of Human-Derived Stem Cells Induced by Biomimetic Calcium Silicate-Reinforced Gelatin Methacrylate Bioink for Odontogenic Regeneration

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