Cerium oxide nanoparticles loaded nanofibrous membranes promote bone regeneration for periodontal tissue engineering

Ren, Xiaobing; Zhou, Yi; Zheng, Kai; Xu, Xuanwen; Yang, Jie; Wang, Xiaoyu; Miao, Leiying; Wei, Hui; Xu, Yan

doi:10.1016/j.bioactmat.2021.05.037

Cited by 74 publications

(69 citation statements)

References 60 publications

(64 reference statements)

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“…Nanomaterials have been widely applied to facilitate the regeneration of damaged oral tissues ( Karakoti et al, 2010 ; Naganuma and Traversa, 2014 ; Kargozar et al, 2018 ; Li et al, 2018 ; Ren et al, 2021 ). For example, nanoparticles have shown advantages in low price and good stability in comparison to growth factors and other bioactive molecules.…”

Section: Engineering Nanofibers With Specific-controlled Structuresmentioning

confidence: 99%

“…For example, nanoparticles have shown advantages in low price and good stability in comparison to growth factors and other bioactive molecules. Among others, nano-hydroxyapatite (n-HA) particles have been extensively used for the fabrication of guided bone regeneration (GBR) membranes due to their structural and functional properties ( Lowe et al, 2020 ; Ren et al, 2021 ). As an important organic constituent part of human bone, n-HA particles provide the scaffold with excellent biocompatibility and bioactivity, thus inducing osteogenic differentiation ( Jin et al, 2019 ).…”

Section: Engineering Nanofibers With Specific-controlled Structuresmentioning

confidence: 99%

“…Scaffolds loaded with functional nanoparticles have also been recognized as promising alternatives for the repair of oral defects. In one study, cerium oxide nanoparticles (CeO 2 NPs), which could provide anti-inflammation, anti-oxidant, and anti-bacterial capabilities, were encapsulated into electrospun PCL/gelatin fibers ( Ren et al, 2021 ). The CeO 2 NPs-loaded fibers promoted better differentiation of human periodontal ligament stem cells to osteoblasts than that using the PCL/gelatin fibers.…”

Section: Engineering Nanofibers With Biofunctionsmentioning

confidence: 99%

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Engineering Electrospun Nanofibers for the Treatment of Oral Diseases

Wang¹,

Liu²,

Zhang³

et al. 2021

Front. Chem.

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With the increase of consumption of high-sugar foods, beverages, tobacco, and alcohol, the incidence rate of oral diseases has been increasing year by year. Statistics showed that the prevalence of oral diseases such as dental caries, dental pulpal disease, and periodontal disease has reached as high as 97% in 2015 in China. It is thus urgent to develop functional materials or products for the treatment of oral diseases. Electrospinning has been a widely used technology that is capable of utilizing polymer solution to generate micro/nano fibers under an appropriate high voltage condition. Owing to their excellent structures and biological performances, materials prepared by electrospinning technology have been used for a wide range of oral-related applications, such as tissue restoration, controlled drug release, anti-cancer, etc. In this regard, this article reviews the application and progress of electrospun nanofibers to various oral diseases in recent years. Firstly, engineering strategies of a variety of nanofiber structures together with their resultant functions will be introduced. Then, biological functions of electrospun nanofibers as well as their applications in the treatment of oral diseases are summarized and demonstrated. Finally, the development viewpoint of functional nanofibers is prospected, which is expected to lay the foundation and propose the direction for further clinical application.

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Section: Engineering Nanofibers With Specific-controlled Structuresmentioning

confidence: 99%

Section: Engineering Nanofibers With Specific-controlled Structuresmentioning

confidence: 99%

Section: Engineering Nanofibers With Biofunctionsmentioning

confidence: 99%

See 1 more Smart Citation

Engineering Electrospun Nanofibers for the Treatment of Oral Diseases

Wang¹,

Liu²,

Zhang³

et al. 2021

Front. Chem.

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“…Nanoceria (nCeO 2 ) were demonstrated in previous studies for tissue regeneration. As a result of their excellent biological properties, antioxidant, anti-inflammatory, and angiogenic proprieties, the nanoceria (nCeO 2 ) were loaded into various polymeric scaffolds for tissue engineering and regeneration [ 28 ]. nCeO 2 incorporated into alginate–gelatin scaffolds were applied to bone regenerations, where they enhanced osteoblast differentiation by scavenging free radicals [ 29 ].…”

Section: Introductionmentioning

confidence: 99%

Dual Spinneret Electrospun Polyurethane/PVA-Gelatin Nanofibrous Scaffolds Containing Cinnamon Essential Oil and Nanoceria for Chronic Diabetic Wound Healing: Preparation, Physicochemical Characterization and In-Vitro Evaluation

et al. 2022

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In this study, a dual spinneret electrospinning technique was applied to fabricate a series of polyurethane (PU) and polyvinyl alcohol–gelatin (PVA/Gel) nanofibrous scaffolds. The study aims to enhance the properties of PU/PVA-Gel NFs loaded with a low dose of nanoceria through the incorporation of cinnamon essential oil (CEO). The as-prepared nCeO2 were embedded into the PVA/Gel nanofibrous layer, where the cinnamon essential oil (CEO) was incorporated into the PU nanofibrous layer. The morphology, thermal stability, mechanical properties, and chemical composition of the produced NF mats were investigated by STEM, DSC, and FTIR. The obtained results showed improvement in the mechanical, and thermal stability of the dual-fiber scaffolds by adding CEO along with nanoceria. The cytotoxicity evaluation revealed that the incorporation of CEO to PU/PVA-Gel loaded with a low dose of nanoceria could enhance the cell population compared to using pure PU/PVA-Gel NFs. Moreover, the presence of CEO could inhibit the growth rate of S. aureus more than E. coli. To our knowledge, this is the first time such nanofibrous membranes composed of PU and PVA-Gel have been produced. The first time was to load the nanofibrous membranes with both CEO and nCeO2. The obtained results indicate that the proposed PU/PVA-Gel NFs represent promising platforms with CEO and nCeO2 for effectively managing diabetic wounds.

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“…With the development of tissue engineering, periodontal regeneration therapy provides more possibilities. Tissue engineering consists of three elements, including “seed cells,” scaffold materials, and an external environment that facilitates cell growth and differentiation [ 4 – 6 ].…”

Section: Introductionmentioning

confidence: 99%

EZH2 Regulates Lipopolysaccharide-Induced Periodontal Ligament Stem Cell Proliferation and Osteogenesis through TLR4/MyD88/NF-κB Pathway

Wang

Tian

Zhang

et al. 2021

Stem Cells International

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Background. Periodontitis induced by bacteria especially Gram-negative bacteria is the most prevalent chronic inflammatory disease worldwide. Emerging evidence supported that EZH2 plays a significant role in the inflammatory response of periodontal tissues. However, little information is available regarding the underlying mechanism of EZH2 in periodontitis. This study is aimed at determining the potential role and underlying mechanism of EZH2 in periodontitis. Methods. The protein levels of EZH2, H3K27ME, p-p65, p-IKB, TLR4, MyD88, Runx2, and OCN were examined by western blot assay. Proliferation was evaluated by CCK8 assay. The levels of TNFα, IL1β, and IL6 were detected by ELISA assay. Migration was detected by wound healing assay. The distribution of p65 was detected by immunofluorescence. The formation of mineralized nodules was analyzed using alizarin red staining. Results. LPS stimulation significantly promoted EZH2 and H3K27me3 expression in primary human periodontal ligament stem cells (PDLSCs). Targeting EZH2 prevented LPS-induced upregulation of the inflammatory cytokines and inhibition of cell proliferation and migration. Furthermore, EZH2 knockdown attenuated the TLR4/MyD88/NF-κB signaling to facilitate PDLSC osteogenesis. Conclusions. Modulation of the NF-κB pathway through the inhibition of EZH2 may offer a new perspective on the treatment of chronic apical periodontitis.

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Cerium oxide nanoparticles loaded nanofibrous membranes promote bone regeneration for periodontal tissue engineering

Cited by 74 publications

References 60 publications

Engineering Electrospun Nanofibers for the Treatment of Oral Diseases

Engineering Electrospun Nanofibers for the Treatment of Oral Diseases

Dual Spinneret Electrospun Polyurethane/PVA-Gelatin Nanofibrous Scaffolds Containing Cinnamon Essential Oil and Nanoceria for Chronic Diabetic Wound Healing: Preparation, Physicochemical Characterization and In-Vitro Evaluation

EZH2 Regulates Lipopolysaccharide-Induced Periodontal Ligament Stem Cell Proliferation and Osteogenesis through TLR4/MyD88/NF-κB Pathway

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