GDF-9 and bFGF enhance the effect of FSH on the survival, activation, and growth of cattle primordial follicles

Tang, Kai-Fu; Li, Xiang; Wu, Canjie; Sang, Lei; Yang, Liguo

doi:10.1016/j.anireprosci.2012.03.009

Cited by 31 publications

(17 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The spontaneous activation of primordial follicles has been known to occur in vitro using the in situ culture system in several species, including mice [18, 69], cattle ([25, 70–74]; Table 1), horses [7], goats [75, 76], and primates [77, 78]. The majority of the bovine primordial follicles cultured in situ may activate within 2 days of culture [25, 27, 70] and reach the secondary stage in 6 [79], 10 [27, 34, 80], or 22 days [74].…”

Section: Reviewmentioning

confidence: 99%

“…The majority of the bovine primordial follicles cultured in situ may activate within 2 days of culture [25, 27, 70] and reach the secondary stage in 6 [79], 10 [27, 34, 80], or 22 days [74]. …”

Section: Reviewmentioning

confidence: 99%

“…Despite the fact that in vitro culture of ovarian tissue is able to develop primordial follicles until primary and secondary stages [27, 34, 74, 80], this technique has not been very effective for follicle maturation. A two-step culture system for bovine [79] and human [81] preantral follicles has been tested recently.…”

Section: Reviewmentioning

confidence: 99%

“…In addition, the balance of stimulatory and suppressive factors dramatically affects the growth of granulosa cells of small preantral follicles in vitro [65]. Among the known major growth factors present in bovine ovarian cells are insulin-like growth factors (IGFs; [96]), fibroblast growth factors (FGFs; [107, 108]), vascular endothelial growth factors (VEGFs; [34]), bone morphogenetic proteins (BMPs), and growth and differentiation factors (GDFs; [74, 109]). The following sections will describe only the growth factors which have been used in in vitro culture systems for bovine preantral follicles.…”

Section: Reviewmentioning

confidence: 99%

See 3 more Smart Citations

In vitro culture of bovine preantral follicles: a review

Araújo

Gastal

Figueiredo

2014

Reprod Biol Endocrinol

View full text Add to dashboard Cite

Preantral follicles are the majority of the ovarian follicle population and their use as a source of homogeneous oocytes for bovine reproductive biotechnologies could result in a substantial advance in this field. However, while in other species embryos and offspring have been produced, in bovine species the results have been limited to the follicular activation of small (primordial) preantral follicles and formation of early antral follicles from large (secondary) preantral follicles after in vitro culture. Therefore, this review will highlight the basic aspects of bovine folliculogenesis by focusing on preantral follicles, the methods of harvesting preantral follicles, the main results from in vitro follicular culture during the last 20 years, and the potential candidate substances (basic supplements, growth factors, and hormones) for improving the efficiency of in vitro follicle growth.

show abstract

Section: Reviewmentioning

confidence: 99%

Section: Reviewmentioning

confidence: 99%

Section: Reviewmentioning

confidence: 99%

Section: Reviewmentioning

confidence: 99%

See 2 more Smart Citations

In vitro culture of bovine preantral follicles: a review

Araújo

Gastal

Figueiredo

2014

Reprod Biol Endocrinol

View full text Add to dashboard Cite

show abstract

“…Garor et al [14] demonstrated the role of bFGF in human PF transition to primary follicles. Tang et al [15] reported that bFGF improved the effect of FSH on PF development and survival during long term culture of bovine ovarian tissue.…”

Section: Introductionmentioning

confidence: 99%

Stimulation of ovarian stem cells by follicle stimulating hormone and basic fibroblast growth factor during cortical tissue culture

et al. 2013

View full text Add to dashboard Cite

BackgroundCryopreserved ovarian cortical tissue acts as a source of primordial follicles (PF) which can either be auto-transplanted or cultured in vitro to obtain mature oocytes. This offers a good opportunity to attain biological parenthood to individuals with gonadal insufficiency including cancer survivors. However, role of various intra- and extra-ovarian factors during PF growth initiation still remain poorly understood. Ovarian biology has assumed a different dimension due to emerging data on presence of pluripotent very small embryonic-like stem cells (VSELs) and ovarian germ stem cells (OGSCs) in ovary surface epithelium (OSE) and the concept of postnatal oogenesis. The present study was undertaken to decipher effect of follicle stimulating hormone (FSH) and basic fibroblast growth factor (bFGF) on the growth initiation of PF during organ culture with a focus on ovarian stem cells.MethodsSerum-free cultures of marmoset (n=3) and human (young and peri-menopausal) ovarian cortical tissue pieces were established. Cortical tissue pieces stimulated with FSH (0.5 IU/ml) or bFGF (100 ng/ml) were collected on Day 3 for histological and molecular studies. Gene transcripts specific for pluripotency (Oct-4A, Nanog), early germ cells (Oct-4, c-Kit, Vasa) and to reflect PF growth initiation (oocyte-specific Gdf-9 and Lhx8, and granulosa cells specific Amh) were studied by q-RTPCR.ResultsA prominent proliferation of OSE (which harbors stem cells) and transition of PF to primary follicles was observed after FSH and bFGF treatment. Ovarian stem cells were found to be released on the culture inserts and retained the potential to spontaneously differentiate into oocyte-like structures in extended cultures. q-RTPCR analysis revealed an increased expression of gene transcripts specific for VSELs, OGSCs and early germ cells suggestive of follicular transition.ConclusionThe present study shows that both FSH and bFGF stimulate stem cells present in OSE and also lead to PF growth initiation. Thus besides being a source of PF, cryopreserved ovarian cortical tissue could also be a source of stem cells which retain the ability to spontaneously differentiate into oocyte-like structures in vitro. Results provide a paradigm shift in the basic understanding of FSH action and also offer a new perspective to the field of oncofertility research.

show abstract