Involvement of Phosphorylated Akt and FOXO3a in the Effects of Growth and Differentiation Factor-9 (GDF-9) on Inhibition of Follicular Apoptosis and Induction of Granulosa Cell Proliferation After In Vitro Culture of Sheep Ovarian Tissue

Monte, Alane Pains Oliveira do; Bezerra, M. É. S.; Menezes, Vanúzia Gonçalves; Gouveia, B.B.; Barberino, Ricássio de Sousa; Lins, T.L.B.G.; Barros, Vanessa Raquel Pinto de; Santos, J.M.S.; Donfack, Nathalie Jiatsa; Matos, Maria Helena Tavares de

doi:10.1007/s43032-020-00409-x

Cited by 8 publications

(2 citation statements)

References 52 publications

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“…Our results also showed that follicular activation has been inhibited by LY294002 in both α-MEM + and 100 pg/ml melatonin treatments. These data suggest that the PI3K pathway could be potentially involved in ovine primordial follicle activation in vitro, which agrees with previous studies in different species (rat: Keating et al, 2009;mouse: Q. Zhao et al, 2014;cat: Thuwanut et al, 2017;sheep: Lins et al, 2021;Monte et al, 2021). The possible explanation for the involvement of the PI3K pathway in follicular activation observed in the α-MEM + is the presence of insulin in the medium, which acts by activating this pathway (Khorami et al, 2015).…”

Section: Discussionsupporting

confidence: 92%

Immunolocalization of melatonin receptor type 1 in the sheep ovary and involvement of the PI3K/Akt/FOXO3a signaling pathway in the effects of melatonin on survival and in vitro activation of primordial follicles

Barberino

Macedo

Lins

et al. 2022

Molecular Reproduction Devel

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This study characterized the expression of melatonin receptor type 1 (MT 1 ) protein in sheep ovaries, evaluated melatonin effects on primordial follicle survival and development after in vitro culture of ovarian tissue and verified the possible involvement of the phosphatidylinositol-3-kinase/protein kinase B/forkhead box O3a (PI3K/Akt/FOXO3a) pathway in the melatonin actions. Ovine ovarian fragments were cultured in α-modified minimum essential medium alone (α-MEM + ) or supplemented with 100, 500, or 1000 pg/ml melatonin for 7 days. PI3K inhibition was performed through pretreatment of ovarian fragments with LY294002. Thereafter, immunohistochemistry was performed to evaluate the expression of cleaved caspase-3, Akt, phosphorylated-Akt, and phosphorylated-FOXO3a (p-FOXO3a). The immunohistochemical localization of the MT 1 receptor protein was documented in sheep preantral and antral follicles. After in vitro culture, 100 pg/ml melatonin showed higher follicular survival and activation than α-MEM + and other melatonin concentrations.After PI3K inhibition, there was an increase in cleaved caspase-3-positive follicles, and a decrease in the primordial follicle activation, Akt phosphorylation, and nuclear exclusion of p-FOXO3a. In conclusion, MT 1 receptor protein is present in the sheep ovary.Furthermore, 100 pg/ml melatonin maintains survival and stimulates activation of primordial follicles through the PI3K/Akt/FOXO3a signaling pathway after in vitro culture of sheep ovarian tissue. K E Y W O R D S hormone receptor, immunohistochemistry, in vitro culture, ovarian cortex, preantral follicle 1 | INTRODUCTION In vitro activation of primordial follicles cultured within the ovary itself is an essential tool to study the early follicle development and optimize the use of the female germ cell pool (Telfer & Zelinski, 2013; J. J. Wang et al., 2017). Studies on in vitro activation of primordial follicles, and subsequent growth and maturation have gained a lot of attention as a potential source of mature eggs for in vitro fertilization and embryo transfer, followed by the delivery of healthy born

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Section: Discussionsupporting

confidence: 92%

Immunolocalization of melatonin receptor type 1 in the sheep ovary and involvement of the PI3K/Akt/FOXO3a signaling pathway in the effects of melatonin on survival and in vitro activation of primordial follicles

Barberino

Macedo

Lins

et al. 2022

Molecular Reproduction Devel

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“…The proliferation and differentiation of granulosa cells are an important part of follicle development [12]. Chronic lowgrade inflammation plays an important role in the pathogenesis of PCOS.…”

Section: Kd Reduced Inflammation and Apoptosis In Ovarian Granulosa C...mentioning

confidence: 99%

Effects of a ketogenic diet on reproductive and metabolic phenotypes in mice with polycystic ovary syndrome

Liu

Yao

et al. 2023

Biology of Reproduction

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Polycystic ovary syndrome (PCOS) is one of the most common female reproductive and metabolic disorders. The ketogenic diet (KD) is a diet high in fat and low in carbohydrate. The beneficial effects of KD intervention have been demonstrated in obese women with PCOS. The underlying mechanisms, however, remain unknown. The aim of the present study was to investigate the effects of a ketogenic diet on both reproductive and metabolic phenotypes of dehydroepiandrosterone (DHEA)-induced PCOS mice. Female C57BL/6 mice were divided into three groups, designated Control, DHEA, and DHEA+KD groups. Mice of both Control and DHEA groups were fed the control diet, whereas DHEA+KD mice were fed a ketogenic diet with 89%(kcal) fat for 3 weeks or 1 week after PCOS mouse model was completed. At the end of the experiment, both reproductive and metabolic characteristics were assessed. Our data show that KD treatment significantly increased blood ketone levels, reduced body weight and random and fasting blood glucose levels in DHEA+KD mice compared with DHEA mice. Glucose tolerance, however, was impaired in DHEA+KD mice. Ovarian functions were improved in some DHEAmice after KD feeding, especially in mice treated with KD for 3 weeks. In addition, inflammation and cell apoptosis were inhibited in the ovaries of DHEA+KD mice. Results from in vitro experiments showed that the main ketone body β-hydroxybutyrate reduced inflammation and cell apoptosis in DHEA-treated KGN cells. These findings support the therapeutic effects of KD and reveal a possible mechanism by which KD improves ovarian functions in PCOS mice.

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Arsenic-Induced Thyroid Hormonal Alterations and Their Putative Influence on Ovarian Follicles in Balb/c Mice

Nalla

et al. 2023

Biol Trace Elem Res

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Involvement of Phosphorylated Akt and FOXO3a in the Effects of Growth and Differentiation Factor-9 (GDF-9) on Inhibition of Follicular Apoptosis and Induction of Granulosa Cell Proliferation After In Vitro Culture of Sheep Ovarian Tissue

Cited by 8 publications

References 52 publications

Immunolocalization of melatonin receptor type 1 in the sheep ovary and involvement of the PI3K/Akt/FOXO3a signaling pathway in the effects of melatonin on survival and in vitro activation of primordial follicles

Immunolocalization of melatonin receptor type 1 in the sheep ovary and involvement of the PI3K/Akt/FOXO3a signaling pathway in the effects of melatonin on survival and in vitro activation of primordial follicles

Effects of a ketogenic diet on reproductive and metabolic phenotypes in mice with polycystic ovary syndrome

Arsenic-Induced Thyroid Hormonal Alterations and Their Putative Influence on Ovarian Follicles in Balb/c Mice

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